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Full-Text Articles in Microbiology
Molecular Padlock Assay Of Crude Plant Leaf Extracts For Detection Of Listeria Monocytogenes, Allison L. Hurlburt
Molecular Padlock Assay Of Crude Plant Leaf Extracts For Detection Of Listeria Monocytogenes, Allison L. Hurlburt
Electronic Theses and Dissertations
A molecular padlock assay was developed and assessed for detection of Listeria monocytogenes that operated in crude plant extracts. The molecular padlock assay was developed by Liu et al. (1996) and modified by Lizardi et al. (1998). We further modified and described a padlock probe that detected L. monocytogenes oligonucleotide, cDNA and genomic DNA containing a 16s rRNA sequence (GenBank Acc. No. X56 153). This technique was effective in the presence of crude potato leaf extracts in contrast to PCR, which failed to detect the presence of L. monocytogenes targets in crude leaf extracts. Sensitivity of the padlock procedure was …
Molecular Characterization Of Zebrafish Interferon, Mx, And Mx Promoter, Stephen Altmann
Molecular Characterization Of Zebrafish Interferon, Mx, And Mx Promoter, Stephen Altmann
Electronic Theses and Dissertations
Type I interferons (IFNs) represent a family of biological molecules whose antiviral, antitumor, and immunomodulatory role is well known. IFNs were first identified in the 1950's and have since been used extensively for the treatment of various cancers, and viral infections. In order to more fully characterize the IFN response, it is often necessary to use animal models. Although the mouse has been used extensively for IFN studies, a lower order vertebrate model is also desirable, as it would provide information about the structure and function of a more ancestral IFN. To this end, herein is described the cloning and …