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Articles 1 - 7 of 7
Full-Text Articles in Microbiology
Examining The Effect Of Varying Dilutions Of Octopamine On The Gut Microbiome Of Lab-Raised And Wild Acheta Domesticus By Brandon Nelson University Of Puget Sound Biology, Brandon Nelson
Summer Research
For my research I am comparing the gut microbiome of lab raised A. Domesticus that are given various levels of Octopamine, a stress hormone, and comparing that to the gut microbiome of wild cricket types. Crickets are a widely used organism across the world for various experiments as both subject and feed. Knowing how the gut microbiome of these crickets is affected by various levels of stress will allow us to better take care of these crickets. This could also help us determine if there are any diseases that lab raised crickets may be susceptible to. A large part of …
Undescribed Oomycete Pathogens On Zostera Marina And Z. Japonica In The Puget Sound, Theresa Proctor
Undescribed Oomycete Pathogens On Zostera Marina And Z. Japonica In The Puget Sound, Theresa Proctor
Summer Research
A diverse range of marine organisms relies on seagrass as a foundational species. However, seagrass populations are declining at an alarming rate and recent analysis of seagrass root microbiomes indicates that oomycetes (fungi-like protists that are notorious terrestrial plant pathogens) infect up to 99% of seagrasses worldwide. Shockingly, very little is known about marine oomycetes and their ecological roles. The Elliott lab has isolated and cultured six oomycete species from eelgrass (Zostera marina) root samples: Phytophthora gemini, aHalophytophthoraspecies discovered by WSU, and four undescribed oomycete species. During the summer of 2018, I generated temperature and salinity growth curves and isolated …
Examining Egg Surface Morphology And Microbial Content Of Sceloporus Virgatus Eggshells, Linnaea Arnett, Stacey Weiss Dr., Mark Martin Dr.
Examining Egg Surface Morphology And Microbial Content Of Sceloporus Virgatus Eggshells, Linnaea Arnett, Stacey Weiss Dr., Mark Martin Dr.
Summer Research
Eggs laid by the Sceloporus virgatus lizard are exposed to a variety of potentially protective microorganisms when passed through the cloaca, the opening at the end of both the reproductive and digestive tracts. Past data have indicated that eggs dissected out of the female before passing through the cloaca often have decreased hatching success, potentially due to the lack of protective bacteria on the dissected eggshell surface. This study examined the microorganisms found on both laid and dissected eggs at 0 weeks of incubation, as well as microbial and fungal content on the eggshells after 3 weeks of incubation in …
Effects Of Sugar-Amendment On Bacterial & Fungal Abundance In Native Vs. Nonnative-Dominated Soils Of A Puget Lowland Prairie, Jessica Wong
Effects Of Sugar-Amendment On Bacterial & Fungal Abundance In Native Vs. Nonnative-Dominated Soils Of A Puget Lowland Prairie, Jessica Wong
Summer Research
Scotch broom is a nonnative plant that has invaded the plant communities of the Pacific Northwest in the United States. It associates with bacteria in the soil to fix nitrogen from the atmosphere, thereby elevating soil nitrogen levels and encouraging the invasion of the community by other nonnative plants like itself. Researchers have used sugar-amendment to decrease soil nitrogen and restore native plant growth. Our study took place in Glacial Heritage Preserve, a Puget lowland prairie that has been invaded by broom in several areas. We aimed to investigate whether sugar-amendment increased or decreased bacterial and fungal abundance in native …
What (Or Who) Lives Inside Scotch Broom Roots?, Lisa Fazzino
What (Or Who) Lives Inside Scotch Broom Roots?, Lisa Fazzino
Summer Research
Scotch broom (Cystis scoparius) is invading the South Puget lowlands, presumably with help from the nitrogen-fixing symbiotic bacteria that are found in its root nodules. With this nitrogen source, Scotch broom is not limited by low nitrogen levels in the soils like many other plants. The goals of our research are 1) to identify the nodulating bacteria of Scotch broom in the Northwest, British Columbia, and Canberra, Australia to explore the diversity of Scotch broom symbionts and 2) to explore the possibility of multiple occupancy in Scotch broom nodules. Using 16s rRNA sequencing, we will identify the bacteria of Scotch …
Exploring The Effects Of C-Amendment On The Soil Microbial Community In A Puget Lowland Prairie, Jessica Wong
Exploring The Effects Of C-Amendment On The Soil Microbial Community In A Puget Lowland Prairie, Jessica Wong
Summer Research
Scotch broom is a non-native plant that has invaded plant communities worldwide. Able to grow on a variety of soil conditions, Scotch broom associates with soil bacteria to fix nitrogen (N) from the atmosphere, elevating soil N and crowding out native plant species by encouraging the growth of non-natives like itself. Adding carbon (C) has been used in previous studies to lower soil N and restore native plant growth. However, little is known about C-amendment’s effects on the soil microbial community that correspond to changes in soil N and plant community composition. Recent studies have shown that C-amendment increases microbial …
Searching For Crp: Investigating Candidate Genes For The Camp Receptor Protein In Bdellovibrio Bacteriovorus, Kat Schmidt
Searching For Crp: Investigating Candidate Genes For The Camp Receptor Protein In Bdellovibrio Bacteriovorus, Kat Schmidt
Summer Research
Bdellovibrio bacteriovorus is a δ-proteobacter with an obligatory predatory lifestyle, consuming a wide variety of Gram-negative bacteria. Its unusual life cycle and genome make it a particularly interesting organism to study. In this experiment, three candidate crp homologs – Bd0446, Bd2590, and Bd2602 – were evaluated by complementation studies in Escherichia coli, and characterized by bioinformatic analysis. Based on the complementation studies, none of these genes function as a crp homolog in E. coli. However, comparative bioinformatic analysis strongly suggests that either Bd2590 or Bd2602 may indeed code for the cyclic AMP receptor protein in Bdellovibrio, and merit further investigation. …