Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Biology (2)
- Genetics and Genomics (2)
- Molecular Genetics (2)
- Other Genetics and Genomics (2)
- Pathogenic Microbiology (2)
-
- Bacteria (1)
- Bacterial Infections and Mycoses (1)
- Bacteriology (1)
- Biochemistry, Biophysics, and Structural Biology (1)
- Bioinformatics (1)
- Cellular and Molecular Physiology (1)
- Diseases (1)
- Environmental Microbiology and Microbial Ecology (1)
- Genomics (1)
- Immunology and Infectious Disease (1)
- Marine Biology (1)
- Medicine and Health Sciences (1)
- Molecular Biology (1)
- Organismal Biological Physiology (1)
- Organisms (1)
- Physiology (1)
- Respiratory Tract Diseases (1)
- Keyword
-
- Pseudomonas aeruginosa (2)
- Acclimatization (1)
- Acute and chronic infection (1)
- Adaptation (1)
- AmpR (1)
-
- Antibiotic resistance (1)
- Biological agents (1)
- Biology (1)
- ChIP-Seq (1)
- Coral bleaching (1)
- DNA (1)
- Detection (1)
- Forensics (1)
- Gene regulatory network (1)
- Global change (1)
- Host pathogen interaction (1)
- Macrophage (1)
- Microarray (1)
- RNA-Seq (1)
- Rab5 (1)
- Real-time qPCR (1)
- Small RNA (1)
- Symbiosis (1)
- Thermal stress (1)
- Transcriptomics (1)
- Virulence (1)
Articles 1 - 4 of 4
Full-Text Articles in Microbiology
Real-Time Qpcr Assay Development For Detection Of Bacillus Thuringiensis And Serratia Marcescens Dna And The Influence Of Complex Microbial Community Dna On Assay Sensitivity, Jonathan Segal
FIU Electronic Theses and Dissertations
Real-time quantitative polymerase chain reaction (real-time qPCR) assays are an effective technique to detect biological warfare agents and surrogate organisms. In my study, primers were designed to detect chromosomal DNA of biological warfare agent surrogates B. thuringiensis and S. marcescens (representing B. anthracis and Y. pestis, respectively) via real-time qPCR. Species-level specificity of the primers was demonstrated through comparisons with a bacterial strain panel and corroborated by qPCR data. Additionally, the primer efficacy was tested when template DNA was spiked into metagenomic DNA extracted from clinical lung microbiome samples. The results showed that while detection of B. thuringiensis or …
Regulation Of Rab5 Gtpase Activity During Pseudomonas Aeruginosa-Macrophage Interaction, Sushmita Mustafi
Regulation Of Rab5 Gtpase Activity During Pseudomonas Aeruginosa-Macrophage Interaction, Sushmita Mustafi
FIU Electronic Theses and Dissertations
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen. Several antibiotic resistant strains of P. aeruginosa are commonly found as secondary infection in immune-compromised patients leaving significant mortality and healthcare cost. Pseudomonas aeruginosa successfully avoids the process of phagocytosis, the first line of host defense, by secreting several toxic effectors. Effectors produced from P. aeruginosa Type III secretion system are critical molecules required to disrupt mammalian cell signaling and holds particular interest to the scientists studying host-pathogen interaction. Exoenzyme S (ExoS) is a bi-functional Type III effector that ADP-ribosylates several intracellular Ras (Rat sarcoma) and Rab (Response to abscisic acid) small GTPases …
Acclimatization Of The Tropical Reef Coral Acropora Millepora To Hyperthermal Stress, Anthony John Bellantuono
Acclimatization Of The Tropical Reef Coral Acropora Millepora To Hyperthermal Stress, Anthony John Bellantuono
FIU Electronic Theses and Dissertations
The demise of reef-building corals potentially lies on the horizon, given ongoing climate change amid other anthropogenic environmental stressors. If corals cannot acclimatize or adapt to changing conditions, dramatic declines in the extent and health of the living reefs are expected within the next half century. The primary and proximal global threat to corals is climate change. Reef-building corals are dependent upon a nutritional symbiosis with photosynthetic dinoflagellates belonging to the group Symbiodinium. The symbiosis between the cnidarian host and algal partner is a stress-sensitive relationship; temperatures just 1°C above normal thermal maxima can result in the breakdown of …
Pseudomonas Aeruginosa Ampr Transcriptional Regulatory Network, Deepak Balasubramanian
Pseudomonas Aeruginosa Ampr Transcriptional Regulatory Network, Deepak Balasubramanian
FIU Electronic Theses and Dissertations
In Enterobacteriaceae, the transcriptional regulator AmpR, a member of the LysR family, regulates the expression of a chromosomal β-lactamase AmpC. The regulatory repertoire of AmpR is broader in Pseudomonas aeruginosa, an opportunistic pathogen responsible for numerous acute and chronic infections including cystic fibrosis. Previous studies showed that in addition to regulating ampC, P. aeruginosa AmpR regulates the sigma factor AlgT/U and production of some quorum sensing (QS)-regulated virulence factors. In order to better understand the ampR regulon, the transcriptional profiles generated using DNA microarrays and RNA-Seq of the prototypic P. aeruginosa PAO1 strain with its isogenic ampR deletion …