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Full-Text Articles in Microbiology

Quantification Of Interactions Between Influenza Hemagglutinin And Host Cell Phosphoinositides By Super-Resolution Microscopy, Matthew T. Parent May 2020

Quantification Of Interactions Between Influenza Hemagglutinin And Host Cell Phosphoinositides By Super-Resolution Microscopy, Matthew T. Parent

Electronic Theses and Dissertations

The influenza viral membrane protein hemagglutinin (HA) forms dense nanoscale clusters on host cell plasma membranes (PM), but the mechanisms that direct HA clustering are not well understood. Previous studies have observed HA associated with actin rich regions of the PM, but there are no known direct interactions between HA and actin. Phosphatidylinositol 4,5-biphosphate (PIP2) is a signaling lipid in the PM which can regulate the actin cytoskeleton, and actin comets initiated by PIP2 are known to be exploited by HA to reach the PM of infected cells. PIP2 is also used by other viruses, such as HIV and Ebola, …


The Effects Of Seasonal Variations In Chemistry And Hydrology On The Microbial Community And Its Sulfide Oxidation Potential In A Naturally Acidic Maine Stream, Raymond C. Kahler Iii Dec 2018

The Effects Of Seasonal Variations In Chemistry And Hydrology On The Microbial Community And Its Sulfide Oxidation Potential In A Naturally Acidic Maine Stream, Raymond C. Kahler Iii

Electronic Theses and Dissertations

Sulfide minerals oxidize through interaction with water and oxygen, releasing hydrogen ions. The process often occurs naturally near metal sulfide deposits, and can be accelerated through mining. Microorganisms accelerate the rate of sulfide oxidation. Acidified streams typically contain high metal concentrations (e.g. aluminum) and microbes in these systems may develop resistances to metal toxicity. Stream flow can affect sulfide oxidation and microbial community structure. Baseflow can influence stream chemistry from interactions with the surrounding bedrock, while stormflow affects stream chemistry and the local microbial community through dilution and addition of microbes transported by runoff. Microbial community composition is affected by …


Reducing Tillage In Small-Scale Permanent Bed Organic Vegetable Production Systems, Jeremiah D. Vallotton May 2018

Reducing Tillage In Small-Scale Permanent Bed Organic Vegetable Production Systems, Jeremiah D. Vallotton

Electronic Theses and Dissertations

The response of field-grown vegetable crops to reduced tillage and mulching in permanent beds was evaluated through measuring crop yields, weed pressure, earthworm counts, and soil basal respiration. Two vegetable crops (“Bush Delicata” squash and “Farao” cabbage) were started in April and May of 2016 and 2017 respectively, transplanted in late June, and harvested on 15-Sep-2016 and 25-Aug-2017. Fruit number and weight of squash, and head weight and feeding damage of cabbage were measured. These results suggest that intensive tillage (8” rototill every year) can be successfully reduced to alternating years of shallow (2”) rototilling and a less intensive form …


Summary Report Of Enhanced Monitoring And Pollution Source Tracking Efforts In The Willard Beach Watershed, Maine, 2012-2016, M. Sims, K. Kaczor Jul 2017

Summary Report Of Enhanced Monitoring And Pollution Source Tracking Efforts In The Willard Beach Watershed, Maine, 2012-2016, M. Sims, K. Kaczor

Maine Sea Grant Publications

Willard Beach is a popular recreation area located in South Portland used primarily by families with young children. Routine monitoring at the beach revealed elevated bacteria levels, prompting the need for enhanced monitoring within the watershed to determine the nature and extent of potential bacteria inputs. Although the waste of domestic and wild animals can contribute to impaired water quality and pose a public health risk, efforts have focused primarily on identifying and removing human sources (e.g. malfunctioning septic systems, faulty sewer lines) of fecal pollution.


Maine Healthy Beaches Program 2016 Annual Report To U.S. Epa, K. Kaczor, M. Sims Jul 2017

Maine Healthy Beaches Program 2016 Annual Report To U.S. Epa, K. Kaczor, M. Sims

Maine Sea Grant Publications

Maine Healthy Beaches (MHB) is managed by the Maine Department of Environmental Protection (ME DEP) and coordinated by the University of Maine Cooperative Extension (UMaine Extension). In 2016, this team worked with 28 local management entities to conduct routine monitoring, assessment, and public notification of water quality conditions for 60 beach management areas spanning Kittery to Mount Desert Island. MHB staff continued to build local capacity for well-informed beach management and to address pollution issues when they arose during the beach season.


Summary Report Of Enhanced Monitoring And Pollution Source Tracking Efforts In Goosefare Brook, Maine, 2012-2016, M. Sims, K. Kaczor Jul 2017

Summary Report Of Enhanced Monitoring And Pollution Source Tracking Efforts In Goosefare Brook, Maine, 2012-2016, M. Sims, K. Kaczor

Maine Sea Grant Publications

The Goosefare Brook (GFB) forms the border between the City of Saco to the south and Town of Old Orchard Beach (OOB) to the north. In response to concerns over water quality in the mouth and adjacent beach water, the Maine Healthy Beaches (MHB) program has supported multi-year enhanced monitoring and pollution source tracking efforts, held Stakeholder Workshops, and more to address impaired water quality throughout the watershed. Over the past five years, the MHB program has focused primarily on paired enterococci and optical brightener samples in OOB’s New Salt Rd. Tributary (NSRT). This work identified widespread bacterial contamination throughout …


2016 Goosefare Brook Mst Summary And Next Steps, M. Sims, K. Kaczor Jan 2017

2016 Goosefare Brook Mst Summary And Next Steps, M. Sims, K. Kaczor

Maine Sea Grant Publications

No abstract provided.


Jordan River Monitoring Report, Ashley Taylor, Anna Farrell Jan 2017

Jordan River Monitoring Report, Ashley Taylor, Anna Farrell

Maine Sea Grant Publications

In congruence with the Maine Department of Marine Resources’ (DMR) efforts to monitor declining water quality in the Jordan River, Frenchman Bay, Maine, the Community Lab at MDI Biological Laboratory implemented regular water quality monitoring at additional sites in the watershed to supplement the work of the DMR. Water samples were collected weekly between June and August of 2017 by staff and students from the Community Lab. Samples were collected via boat and foot to reach additional intermittent streams and smaller tributaries that feed into the Jordan River. Samples were analyzed for Enterococcus bacteria, optical brighteners, and salinity. A goal …


Investigating High Speed Localization Microscopy Through Experimental Methods, Data Processing Methods, And Applications Of Localization Microscopy To Biological Questions, Andrew J. Nelson Dec 2016

Investigating High Speed Localization Microscopy Through Experimental Methods, Data Processing Methods, And Applications Of Localization Microscopy To Biological Questions, Andrew J. Nelson

Electronic Theses and Dissertations

Fluorescence Photoactivation Localization Microscopy(FPALM) and other super resolution localization microscopy techniques can resolve structures with nanoscale resolution. Unlike techniques of electron microscopy, they are also compatible with live cell and live animal studies, making FPALM and related techniques ideal for answering questions about the dynamic nature of molecular biology in living systems. Many processes in biology occur on rapid sub second time scales requiring the imaging technique to be capable of resolving these processes not just with a high enough spatial resolution, but with an appropriate temporal resolution. To that end, this Dissertation in part investigates high speed FPALM as …


U.S.-Korea Cooperative Research: Carbon Monoxide As A Substrate For Microbial Maintenance, Gary M. King Dec 2007

U.S.-Korea Cooperative Research: Carbon Monoxide As A Substrate For Microbial Maintenance, Gary M. King

University of Maine Office of Research Administration: Grant Reports

Bacteria play an important role in the global budget of carbon monoxide (CO). Largely unknown bacterial populations in soils and the water column of aquatic systems oxidize hundreds of teragrams per year, or about 10%-20% of the estimated annual flux to the atmosphere. In spite of their biogeochemical significance, relatively little is known about the identity of CO-oxidizing populations active in situ, their phylogenic and physiological diversity or the importance of CO as substrate for their basic metabolic needs. of CO oxidizers. It is clear that CO at high concentrations (> 1000 ppm) can serve as a sole source of …


Microbial Observatories: Kilauea Volcano Observatory For Carbon Monoxide-Oxidizing Bacteria, Gary M. King Jul 2007

Microbial Observatories: Kilauea Volcano Observatory For Carbon Monoxide-Oxidizing Bacteria, Gary M. King

University of Maine Office of Research Administration: Grant Reports

Volcanic activity regularly creates new landforms from deposits of tephra, ash and lava. These initially sterile, pristine deposits undergo a range of physical, chemical and biological transformations that lead in some cases to diverse, complex ecosystems such as Hawaiian rainforests. Recent activity by the Kilauea volcano has created unique opportunities to understand the timing and controls of complex ecosystem development, and to analyze the roles of microbes as pioneering colonists that contribute to plant development and succession. The newly established Kilauea Volcano Microbial Observatory will compare the diversity and activity of carbon monoxide-oxidizing bacteria colonizing two different deposits currently 45 …