Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Publication
- Publication Type
Articles 1 - 4 of 4
Full-Text Articles in Microbiology
Fungal Solid-State Fermentation And Various Methods Of Enhancement In Cellulase Production, Li Wan Yoon, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Fungal Solid-State Fermentation And Various Methods Of Enhancement In Cellulase Production, Li Wan Yoon, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Teck Nam Ang Dr.
Cellulase serves vast applications in the industries of biofuel, pulp and paper, detergent and textile. With the presence of its three components i.e. endoglucanase, exoglucanase and β-glucosidase, the enzyme can effectively depolymerize the cellulose chains in lignocellulosic substrate to produce smaller sugar units that consists of cellobiase and glucose. Fungi are the most suitable cellase producers attributing to its ability to produce a complete cellulase system. Solid state fermentation (SSF) by fungi is a preferable production route for cellulase as it imposes lower cost and enables the production of cellulase with higher titre. This article gives an overview on the …
Development Of A Novel Inoculum Preparation Method For Solid-State Fermentation - Cellophane Film Culture (Cfc) Technique, Teck Nam Ang, Gek Cheng Ngoh, Adeline Seak May Chua
Development Of A Novel Inoculum Preparation Method For Solid-State Fermentation - Cellophane Film Culture (Cfc) Technique, Teck Nam Ang, Gek Cheng Ngoh, Adeline Seak May Chua
Teck Nam Ang Dr.
This study reports a user-friendly technique in the preparation of fungal inoculum intended for solid-state fermentation (SSF) – Cellophane Film Culture (CFC) technique. This technique uses cellophane film-overlaid agar plates to facilitate the separation of fungal biomass. The findings showed that inoculum of P. sajor-caju produced is viable, and it was confirmed by the presence of laccase enzyme activity in SSF of rice husk. The correlation between fungal dry and wet weights (r2 = 0.9329) provides an accurate estimation of fungal dry weight from its wet weight during inoculum preparation. Besides, this technique does not require a strict sterile handling …
A Quantitative Method For Fungal Ligninolytic Enzyme, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
A Quantitative Method For Fungal Ligninolytic Enzyme, Teck Nam Ang Dr., Gek Cheng Ngoh Dr, Adeline Seak May Chua Dr
Teck Nam Ang Dr.
Screening is conducted to select a fungus with desired characteristics intended for various applications, e.g. bioremediation and enzyme production. A qualitative method was used in this study for screening of fungal ligninolytic enzymes activities. The fungal ligninolytic activity was correlated with its growth from the screenings to identify a suitable fungus for solid substrate fermentation. Four strains of fungi, namely Phanerochaete chrysosporium, Pycnoporus sanguineus, Phlebia radiata and Pleurotus sajor-caju, were screened for their ligninolytic enzymes activities using guaiacol and Remazol Brilliant Blue-R (RBB-R) as screening reagents. The screenings were conducted at both the optimal growth temperature of each fungus and …
Saccharomyces Cerevisiae-Based Molecular Tool Kit For Manipulation Of Genes From Gram-Negative Bacteria, Robert M. Q. Shanks, Nicky C. Caiazza, Shannon M. Hinsa, Christine M. Toutain, George A. O'Toole
Saccharomyces Cerevisiae-Based Molecular Tool Kit For Manipulation Of Genes From Gram-Negative Bacteria, Robert M. Q. Shanks, Nicky C. Caiazza, Shannon M. Hinsa, Christine M. Toutain, George A. O'Toole
Dartmouth Scholarship
A tool kit of vectors was designed to manipulate and express genes from a wide range of gram-negative species by using in vivo recombination. Saccharomyces cerevisiae can use its native recombination proteins to combine several amplicons in a single transformation step with high efficiency. We show that this technology is particularly useful for vector design. Shuttle, suicide, and expression vectors useful in a diverse group of bacteria are described and utilized. This report describes the use of these vectors to mutate clpX and clpP of the opportunistic pathogen Pseudomonas aeruginosa and to explore their roles in biofilm formation and surface …