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Full-Text Articles in Microbiology
Dual-Action Hygienic Coatings: Benefits Of Hydrophobicity And Silver Ion Release And Surface Analysis, Niall Stobie, Brendan Duffy, John Colreavy, Patrick Mchale, Steven Hinder, Declan Mccormack
Dual-Action Hygienic Coatings: Benefits Of Hydrophobicity And Silver Ion Release And Surface Analysis, Niall Stobie, Brendan Duffy, John Colreavy, Patrick Mchale, Steven Hinder, Declan Mccormack
Articles
Coatings that demonstrate reduced attachment of crystalline precipitates and the medical device colonising Staphylococcus epidermidis were prepared by the immobilisation of silver doped perfluoropolyether–urethane siloxane thin films on glass substrates. The presence of stratified hydrophobic perfluoropolyether groups protects the coating surface from the attachment of crystalline hydrophilic species such as chlorides and phosphates, whilst silver ion release inhibited attachment of S. epidermidis and subsequent biofilm formation in vitro. The release of silver ions protects the perfluoro groups from the hydrophobic interactions of S. epidermidis cells, which can reduce the hydrophobicity of the protective coating. These coatings also exhibited significant antibacterial …
An Evaluation Of Miru-Vntr Analysis And Spoligotyping For Genotyping Of Mycobacterium Bovis Isolates And A Comparison With Rflp Typing., Joanne Mclernon, Eamonn Costello, Orla Flynn, Gillian Madigan, Fergus Ryan
An Evaluation Of Miru-Vntr Analysis And Spoligotyping For Genotyping Of Mycobacterium Bovis Isolates And A Comparison With Rflp Typing., Joanne Mclernon, Eamonn Costello, Orla Flynn, Gillian Madigan, Fergus Ryan
Articles
Strain typing of Mycobacterium bovis isolates based on the variable-number tandem repeats of mycobacterial interspersed repetitive units (MIRU-VNTR) and on spoligotyping was evaluated in this study and these typing methods were compared with restriction fragment length polymorphism (RFLP) typing. A total of 386 M. bovis isolates from cattle, badgers and deer in the Republic of Ireland that had previously been typed by IS6110, polymorphic GC-rich sequence (PGRS) and direct repeat (DR) RFLP were included in the study. Spoligotyping and analysis of six VNTR loci (2163a, 2163b, 2165, 4052, 2996 and 1895) was performed on the samples. RFLP was the method …