Marine Biology Commons^™

Generation Of Germ-Free /Ciona Intestinalis For Studies Of Gut-Microbe Interactions, Brittany A Leigh, Assunta Liberti, Larry J Dishaw

Marine Science Faculty Publications

Microbes associate with animal hosts, often providing shelter in a nutrient-rich environment. The gut, however, can be a harsh environment with members of the microbiome settling in distinct niches resulting in more stable, adherent biofilms. These diverse communities can provide orders of magnitude more gene products than the host genome; selection and maintenance of a functionally relevant and useful microbiome is now recognized to be an essential component of homeostasis. Germ-free (GF) model systems allow dissection of host-microbe interactions in a simple and direct way where each member of the symbiosis can be studied in isolation. In addition, because immune …

Flight Of The Freshwater Fish, Michael H. Wilson

Capstones

Michael H. Wilson

Capstone Abstract

December 27, 2016

Flight of the Freshwater Fish

The Hudson River provides for millions of people as a path for commercial and private transportation, a source of food and energy, and perhaps most importantly for many living in the tri-state area as a destination for recreation and relaxation. The most overlooked feature of the river is how the wildlife shows clear signs of a changing climate and rapid environmental response to the impacts of global warming on the river.

Entire populations of fish species in the lower Hudson have been forced to leave the river …

Review Of The Cultivation Program Within The National Alliance For Advanced Biofuels And Bioproducts, Peter J. Lammers, Michael Huesemann, Wiebke Boeing, Daniel B. Anderson, Robert G. Arnold, Xuemei Bai, Manish Bhole, Yalini Brhanavan, Louis Brown, Jola Brown, Judith K. Brown, Stephen Chisholm, C. Meghan Downes, Scott Fulbright, Yufeng Ge, Jonathan E. Holladay, Balachandran Ketheesan, Avinash Khopkar, Ambica Koushik, Paul Laur, Babetta L. Marrone, John B. Mott, Nagamany Nirmalakhandan, Kimberly L. Ogden, Ronald L. Parsons, Juergen Polle, Randy D. Ryan, Tzachi Samocha, Richard T. Sayre, Mark Seger, Thinesh Selvaratnam, Ruixiu Sui, Alex Thomasson, Adrian Unc, Wayne Van Vorrhies, Peter Waller, Yao Yao, José A. Olivares

Publications and Research

The cultivation efforts within the National Alliance for Advanced Biofuels and Bioproducts (NAABB)were developed to provide four major goals for the consortium, which included biomass production for downstream experimentation, development of new assessment tools for cultivation, development of new cultivation reactor technologies, and development of methods for robust cultivation. The NAABB consortium test beds produced over 1500 kg of biomass for downstream processing. The biomass production included a number of model production strains, but also took into production some of the more promising strains found through the prospecting efforts of the consortium. Cultivation efforts at large scale are intensive and …

Behavioral Effects Of Envrionmental Enrichment Devices Of Tursiops Truncatus, Stephanie Maiorano

Honors Projects

Many studies on environmental enrichment devices have been conducted throughout the years on both terrestrial and marine animals in captivity. However, little is known on the effect that submergible enrichment devices have on cetaceans in captivity. This study examines two male Atlantic bottlenose dolphins (Tursiops truncatus), Kaiko’o and Nalu, at Sea Life Park, Hawai’i and their interaction with the Magic Box: a device consisting of a submergible vegetable crate weighed down with a weight, flipped upside down with fish underneath. A total of 7 trials were conducted, with data collection on their surfacing behavior before and after the …

An Evaluation Of The Seagrass Habitat In North Biscayne Bay, Florida, In Relation To A Changing Environment And Urbanization In The Port Of Miami Harbor Basin 2005-2011, Sara M. Jarossy

HCNSO Student Theses and Dissertations

Seagrass habitats in South Florida are exceptionally valuable. They play an important ecological role in the coastal environment by stabilizing sediment, providing habitat for other species and supporting a whole food web. The availability of light and nutrients in aquatic ecosystems are the driving factors behind seagrass distribution. Water quality has been known to influence the abundance, distribution and composition of seagrass beds. South Florida has extensive diverse coastal communities. Throughout its human development dramatic changes have occurred in its natural ecosystems. In South Florida, many examples of seagrass habitat loss are documented, with a variety of contributing factors. The …

A Review Of Present And Alternative Lionfish Controls In The Western Atlantic, Adam Rittermann

HCNSO Student Capstones

Lionfish (Pterois volitans and Pterois miles) are the first recorded invasive piscivore in the Caribbean and have become a threat to native species. As generalist consumers, lionfish have a broad diet and reduce prey and competitor abundance and juvenile recruitment. To confront this problem, this paper serves to review all of the current and alternative future controls available to manage lionfish populations in the Western Atlantic and determine where focus is lacking. Derby and cull efforts are the only management efforts in place and are not effective in their current state as these local events have short-term benefits, …

Using Gis To Predict Cetacean Strandings Related To Harmful Algal Blooms, Jessica L. Boyd

HCNSO Student Capstones

Exposure to harmful algal bloom (HAB) toxins, such as Karenia brevis, has been linked to cetacean strandings and mortalities. Biological and environmental data from a bottlenose dolphin (Tursiops truncatus) Unusual Mortality Event (UME) in 2004 are compared to those from pilot whale (Globicephala macrorhynchus) mass stranding events in 2013-2014 in western Florida. Geographical Information Systems (GIS) maps have been created by overlaying stranding locations and select K. brevis presence data in order to identify common spatial and temporal trends. Preliminary results indicate that elevated K. brevis levels (>10,000 cells/L) in Sarasota Bay during January-February and November may …

Population Demographics And Sexual Reproduction Potential Of The Pillar Coral, Dendrogyra Cylindrus, On The Florida Reef Tract, Lystina Kabay

HCNSO Student Theses and Dissertations

The pillar coral, Dendrogyra cylindrus, has been commonly described as widely distributed, but rare throughout its geographical range in the Caribbean. Having recently been listed as Threatened under the US Endangered Species Act, an understanding of population status is needed to promote species conservation and population recovery. Previous to this study the status of the pillar coral population in the state waters of Florida, U.S.A, was relatively unknown primarily due to few colonies being recorded and no comprehensive summary of population abundance, distribution or health being completed. Along with various environmental and anthropogenic factors affecting the pillar coral population …

Towards Quantitative Viromics For Both Double-Stranded And Single-Stranded Dna Viruses., Simon Roux, Natalie E Solonenko, Vinh T Dang, Bonnie T Poulos, Sarah M Schwenck, Dawn B Goldsmith, Maureen L Coleman, Mya Breitbart, Matthew B Sullivan

Marine Science Faculty Publications

BACKGROUND: Viruses strongly influence microbial population dynamics and ecosystem functions. However, our ability to quantitatively evaluate those viral impacts is limited to the few cultivated viruses and double-stranded DNA (dsDNA) viral genomes captured in quantitative viral metagenomes (viromes). This leaves the ecology of non-dsDNA viruses nearly unknown, including single-stranded DNA (ssDNA) viruses that have been frequently observed in viromes, but not quantified due to amplification biases in sequencing library preparations (Multiple Displacement Amplification, Linker Amplification or Tagmentation).

METHODS: Here we designed mock viral communities including both ssDNA and dsDNA viruses to evaluate the capability of a sequencing library preparation …

Coral Recovery On Phase-Shifted Reefs Depend Upon The Type Of Macroalgae Present, Justin N. Voss

HCNSO Student Theses and Dissertations

The Florida Keys experienced some of the most drastic transitions from coral to macroalgae dominated states, known as phase-or regime-shifts, of any reefs in the Caribbean. Macroalgae on coral reefs lower coral recruitment by deterring coral settlement either directly through competition or indirectly by changing the chemical environment near the benthos. With evidence of species-specific interactions to coral-macroalgae competition, the type of macroalgae on a phase-shifted coral reef might be more important than just identifying a reef transition. To answer this question, I tested the effect of Laurencia intricata (a macroalgae related to the settlement inducing crustose coralline algae) and …

Effects Of 17 Β-Estradiol And Progesterone On Acropora Cervicornis And Porites Astreoides Growth And Reproduction, Joshua L. Stocker

HCNSO Student Theses and Dissertations

Reef-building coral populations throughout the world are being threatened by numerous stressors and continue to decline. As potent endocrine-disrupting compounds, exogenous sex steroid contamination has been a largely overlooked stressor to corals. Previous research indicates these compounds are prevalent in marine environments, fluctuate annually along with reproductive cycles, can bioaccumulate, and have had variable effects on growth and reproduction in several cnidarian species. This project had three primary objectives: (1) establish environmental estradiol and progesterone concentrations in Broward County and lower Florida Keys reef environments, (2) conduct 17 β-estradiol and progesterone larval assays on P. astreoides larvae to determine the …

The Vertical And Horizontal Distribution Of Deep-Sea Crustaceans Of The Order Euphausiacea (Malacostraca: Eucarida) From The Northern Gulf Of Mexico With Notes On Reproductive Seasonality., Charles Douglas Fine

HCNSO Student Theses and Dissertations

The vertical and horizontal distributions of Euphausiacea in the northern Gulf of Mexico, including the location of the Deepwater Horizon oil spill, were analyzed from 340 trawl samples collected between April-June, 2011. This study is the first comprehensive survey of euphausiid distributions from depths deeper than 1000 m in the Gulf of Mexico and included stratified sampling from five discrete depth ranges (0-200 m, 200-600 m, 600-1000 m, 1000-1200 m, and 1200-1500 m). In addition, this study encompasses the region heavily impacted by the Deepwater Horizon oil spill. Data presented here could potentially be used in ecosystem models investigating trophic …

Trophic Ecology Of Green Turtles (Chelonia Mydas) From Dry Tortugas National Park, Florida, David C. Roche

HCNSO Student Theses and Dissertations

Located 100 km west of Key West, Florida, Dry Tortugas National Park (DRTO) is a largely untouched subtropical marine ecosystem that serves as an important developmental habitat, nesting ground, and foraging area for several species of sea turtles, including green turtles. The Park supports a recovering population of green turtles comprised of resident juveniles, subadults, and adults of both sexes; nesting females include residents and migrating females that only return to nest. Stable isotope analysis has been applied widely to describe the trophic ecology of green turtles, from urbanized bays with significant anthropogenic input, to relatively pristine ecosystems with healthy …

Cows - Clean Ocean Wave Sculpture, Kim Bernard

Artist in Residence: Kim Bernard

Description and images from Kim Bernard's University of New England Artist in Residence fall semester 2016 culminating project. Kim worked with UNE students and faculty to gather ocean debris and weave it into a permanent art installment at UNE's Arthur P. Girard Marine Science Center.

Examining The Combined Effects Of Dissolved Oxygen, Temperature, And Body Size On The Physiological Responses Of A Model Macrobenthic Polychaete Species, Capitella Teleta, Kelsey Burns Gillam

Dissertations

While the scientific community is in consensus that coastal systems are threatened by climate change, few climate change studies test the effects of more than one variable directly related to climate change. The dissolved oxygen (DO) levels of the ocean are currently subject to both global warming and eutrophication; 94% of all hypoxia zones are expected to experience >2°C increase by 2035. This dissertation aims to examine how a model organism responds to simultaneous thermal and DO stress involving four levels of DO (100%, 70%, 50%, and 20%) saturation and three temperatures (15°C, 20°C, and 25°C).

The polychaete, Capitella teleta …

Temporal Variability Of Diapycnal Mixing In The Northern South China Sea, Hui Sun, Qingxuan Yang, Wei Zhao, Xinfeng Liang, Jiwei Tian

Marine Science Faculty Publications

Temporal variability of diapycnal mixing over 7 months in the northern South China Sea was examined based on McLane Moored Profiler observations from 850 to 2200 m by employing a finescale parameterization. Intensified diffusivity exceeding the order of 10⁻³ m²/s in magnitude was found over the first half of October 2014, and from 2 December 2014 to 21 January 2015 (a typical wintertime). Strong internal tides and winds in winter were the likely candidates for the high‐level diapycnal mixing in winter. As for the enhanced mixing during October 2014, we suspect the generation of near‐bottom near‐inertial waves …

Molecular Measurement Of Toxicity In Fish; Case Examples And Policy Implications, Jessica A. Freedman

Senior Honors Projects

Stormwater and oil are common urban contaminants that can be harmful to fish species. One way of recognizing exposed and impaired fish is by monitoring gene expression and gene induction. This study focused on the identification and validation of reference genes for measuring contaminant-induced changes in gene expression due to urban influence. In this study, reference genes (which are genes used to normalize data and remain consistent in varying exposures regardless of organism and tissue type) were established. Six genes were identified as reference genes (ef1a, wdtc1, mtm1, spop, rxrba and tuba1) from a longer list of potential …

Gulf Sturgeon (Acipenser Oxyrinchus Desotoi) Pre-Restoration Occupancy Patterns On Ship Island, Mississippi Sound With An Evaluation Of Designated Critical Habitat Use By Eastern And Western Population Segments, Page Elizabeth Vick

Master's Theses

Critical spawning and feeding habitat was designated for federally threatened, anadromous Gulf Sturgeon (GS) to aid in population recovery. This study examined GS occupancy, habitat use, and movement through critical habitat monitored by the Ship Island (SI) acoustic array during overwintering periods from 2011 to 2015 prior to MsCIP SI restoration. An occupancy index analyzed patterns of spatial and temporal habitat use of both western and eastern population segments (WPS and EPS, respectively) of GS on the SI array. The ends of SI along with the passes and cuts of the island, especially Dog Keys Pass (DKP), were occupied by …

Bloom Or Bust: Retrospective Analysis Of The Giant Jellyfish, Nemopilema Nomurai (Scyphozoa: Rhizostomeae), Ecology In The East Asian Marginal Seas, Naomi Yoder

Master's Theses

The Giant Jellyfish, Nemopilema nomurai, is a large-bodied bloom-forming jellyfish that occurs in the semi-enclosed basins of the East Asian Marginal Seas. N. nomurai has bloomed more frequently in the past 20 years than in any period previously recorded. In Japan, recent N. nomurai blooms were responsible for millions of dollars in lost revenue and equipment damage to commercial fisheries alone. This study statistically analyzed 21 environmental factors in connection with N. nomurai occurrence (using occurrence as a proxy for blooms). Eight factors resulted in a statistically significant or marginally significant (p ≤ 0.10), linear or non-linear relationship with …

A Tale Of Two Antennules: The Performance Of Crab Odor-Capture Organs In Air And Water, Lindsay D. Waldrop, Laura A. Miller, Shilpa Khatri

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Odour capture is an important part of olfaction, where dissolved chemical cues (odours) are brought into contact with chemosensory structures. Antennule flicking by marine crabs is an example of discrete odour capture (sniffing) where an array of chemosensory hairs is waved through the water to create a flow–no flow pattern based on a narrow range of speeds, diameters of and spacings between hairs. Changing the speed of movement and spacing of hairs at this scale to manipulate flow represents a complicated fluid dynamics problem. In this study, we use numerical simulation of the advection and diffusion of a chemical gradient …

Effect Of Oxygen-Limiting Tidal Conditions On Muscle Metabolism And Structure In The Giant Acorn Barnacle, Balanus Nubilus, Katie O. Grady

Master's Theses

Crustacean muscle fibers are some of the largest cells in the animal kingdom, with fiber diameters in the giant acorn barnacle (Balanus nubilus) exceeding 3 mm. Sessile animals with extreme muscle sizes and that live in the hypoxia-inducing intertidal zone – like B. nubilus – represent ideal models for probing the effects of oxygen limitation on muscle cells. We investigated changes in metabolism and structure of B. nubilus muscle in response to: normoxic immersion, anoxic immersion, or air emersion, for acute (6h) or chronic (6h exposures twice daily for 2wks) time periods. Following exposure, we immediately measured hemolymph …

Global Ocean Data Science Ocg 250, Joanna Burkhardt

Library Impact Statements

No abstract provided.

Ports, Prosperity, And Pests: Assessing The Threat Of Aquatic Invasive Species Introduced By Maritime Shipping Activity In Cuba, Charleen O'Brien

HCNSO Student Theses and Dissertations

Aquatic invasive species (AIS) are biological pollutants that cause detrimental ecological, economic, and sociological effects on non-native communities. With increasing globalization through maritime trade, coastal ports are vulnerable to AIS introductions transported by commercial vessels. As Cuba’s Port Mariel becomes a competitive transshipment hub within the Caribbean, it is essential to identify the potential threat that AIS may pose with a likely increase in shipping activity. It is equally important to understand the status of established AIS in Cuba and control measures presently being implemented by the country. This information can provide guidance for establishing or improving Cuban AIS preventative …

First Occurrence Of The Invasive Hydrozoan Gonionemus Vertens A. Agassiz, 1862 (Cnidaria: Hydrozoa) In New Jersey, Usa, John J. Gaynor, Paul A.X. Bologna, Dena J. Restaino, Christie Barry

Department of Biology Faculty Scholarship and Creative Works

Gonionemus vertens A. Agassiz, 1862 is a small hydrozoan native to the Pacific Ocean. It has become established in the northern and southern Atlantic Ocean as well as the Mediterranean Sea. We report on the first occurrence of this species in estuaries in New Jersey, USA,and confirm species identification through molecular sequence analysis. Given the large number of individuals collected, we contend that this is a successful invasion into this region with established polyps. The remaining question is the vector and source of these newly established populations.

Variability Of Particle Size Distributions In The Bohai Sea And The Yellow Sea, Zhongfeng Qui, Deyong Sun, Chuanmin Hu, Sengquiang Wang, Lufei Zheng, Yu Huang, Tian Peng

Marine Science Faculty Publications

Particle size distribution (PSD) is an important parameter that is relevant to many aspects of marine ecosystems, such as phytoplankton functional types, optical absorption and scattering from particulates, sediment fluxes, and carbon export. However, only a handful of studies have documented the PSD variability in different regions. Here, we investigate the PSD properties and variability in two shallow and semi-enclosed seas (the Bohai Sea (BS) and Yellow Sea (YS)), using in situ laser diffraction measurements (LISST-100X Type C) and other measurements at 79 stations in November 2013. The results show large variability in particle concentrations (in both volume and number …

Development Of A Risk Assessment Framework To Predict Invasive Species Establishment For Multiple Taxonomic Groups And Vectors Of Introduction, Alisha D. Davidson, Abigail J. Fusaro, Rochelle A. Sturtevant, Edward S. Rutherford, Donna R. Kashian

Biological Sciences Faculty Research Publications

A thorough assessment of aquatic nonindigenous species’ risk facilitates successful monitoring and prevention activities. However, species- and vector-specific information is often limited and difficult to synthesize across a single risk framework. To address this need, we developed an assessment framework capable of estimating the potential for introduction, establishment, and impact by aquatic nonindigenous species from diverse spatial origins and taxonomic classification, in novel environments. Our model builds on previous approaches, while taking on a new perspective for evaluation across species, vectors and stages to overcome the limitations imposed by single species and single vector assessments. We applied this globally-relevant framework …

Fluctuating Asymmetry In Two Common Freshwater Fishes As A Biological Indicator Of Urbanization And Environmental Stress Within The Middle Chattahoochee Watershed, William I. Lutterschmidt, Samantha L. Martin, Jacob F. Schaefer

Faculty Publications

Deviations in bilateral symmetry or fluctuating asymmetry of an organism may result under environmental stressors that reduce developmental homeostasis and stability. Anthropogenic stressors such as increased urbanization can negatively impact environmental quality of aquatic ecosystems. Researchers have stressed the value in finding easy, accurate and inexpensive methods for assessing potential stress within ecosystems. Here we use fluctuating asymmetry (FA) as a useful quantitative tool in assessing the environmental quality and potential urban-based stressors within eight creeks of the Bull and Upatoi Creeks Watershed within the larger watershed of the Middle Chattahoochee. Using Geographic Information System (GIS), we characterize land-use patterns …

Condition Of Larval Spanish Mackerel Scomberomorus Maculatus In Relation To The Deepwater Horizon Oil Spill, John Timothy Ransom, J.E. Filbrun, Frank J. Hernandez Jr.

Faculty Publications

The Deepwater Horizon oil spill (DWHOS) coincided with the pelagic larval stages of many valued commercial and recreational fishes in the northern Gulf of Mexico. Larval fish survival and eventual recruitment into adult populations may have been impacted directly through toxicity or indirectly through changes in the planktonic food web caused by the release of oil and chemical dispersants during the DWHOS event. Using samples from a long-term ichthyoplankton survey off the coast of Alabama, USA, in a region impacted by the DWHOS, the abundance and condition of larval Spanish mackerel Scomberomorus maculatus were compared during summer months in years …

The Skp1 Homologs Skr-1/2 Are Required For The Caenorhabditis Elegans Skn-1 Antioxidant/Detoxification Response Independently Of P38 Mapk., Cheng-Wei Wu, Andrew Deonarine, Aaron Przybysz, Kevin Strange, Keith P. Chloe

Molecular Biosciences Faculty Publications

SKN-1/Nrf are the primary antioxidant/detoxification response transcription factors in animals and they promote health and longevity in many contexts. SKN-1/Nrf are activated by a remarkably broad-range of natural and synthetic compounds and physiological conditions. Defining the signaling mechanisms that regulate SKN-1/Nrf activation provides insights into how cells coordinate responses to stress. Nrf2 in mammals is regulated in part by the redox sensor repressor protein named Keap1. In C. elegans, the p38 MAPK cascade in the intestine activates SKN-1 during oxidative stress by promoting its nuclear accumulation. Interestingly, we find variation in the kinetics of p38 MAPK activation and tissues with …

Newsroom: The Legal Impact Of Marine Debris 10-21-2016, Roger Williams University School Of Law

Life of the Law School (1993- )

No abstract provided.