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Marine Biology Commons

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Full-Text Articles in Marine Biology

Number 35 (August 1997), Southern Fishes Council Aug 1997

Number 35 (August 1997), Southern Fishes Council

Southeastern Fishes Council Proceedings

(August 1997) - Observational notes on the spawning behavior of the blue shiner (Cyprinella caerulea) and the holiday darter (Etheostoma brevirostrum), two rare fishes of the Conasauga River, Georgia and Tennessee. By Carol E. Johnston and J.R. Shute

Fish species composition and associations in a section of Morganfork Creek, southwestern Mississippi. By Mark D. Farr

Minutes, Business Meeting, 22nd Annual Meeting, Southeastern Fishes Council

Regional Southeastern Fishes Council Reports


Use Of Fluorescence Microscopy In An Assay Of Sperm Density For The Gorgonian Coral, Plexaura Kuna, Timothy D. Swain, K. Kim, H. R. Lasker Jun 1997

Use Of Fluorescence Microscopy In An Assay Of Sperm Density For The Gorgonian Coral, Plexaura Kuna, Timothy D. Swain, K. Kim, H. R. Lasker

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

The density of sperm in the water column during the broadcast spawning events of marine invertebrates is often undetermined or reported in terms of fertilization potential. The density of sperm during such events can be determined by directly counting numbers of spermatozoa using a modification of the acridine orange direct count (AODC) technique for enumerating bacteria. A number of variables in the handling and processing of samples may bias estimates. Sample collection in glass and rapid fixation and filtration are necessary for reproducible estimates. Once filtered, samples are stable for many months, and counts on filters that were poorly stained …