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Full-Text Articles in Marine Biology
Dynamics Of Marine Bacterial Community Diversity Of The Coastal Waters Of The Reefs, Inlets, And Wastewater Outfalls Of Southeast Florida, Alexandra Mandina Campbell, Jay Fleisher, Christopher D. Sinigalliano, James R. White, Jose V. Lopez
Dynamics Of Marine Bacterial Community Diversity Of The Coastal Waters Of The Reefs, Inlets, And Wastewater Outfalls Of Southeast Florida, Alexandra Mandina Campbell, Jay Fleisher, Christopher D. Sinigalliano, James R. White, Jose V. Lopez
Marine & Environmental Sciences Faculty Articles
Coastal waters adjacent to populated southeast Florida possess different habitats (reefs, oceanic inlets, sewage outfalls) that may affect the composition of their inherent microbiomes. To determine variation according to site, season, and depth, over the course of 1 year, we characterized the bacterioplankton communities within 38 nearshore seawater samples derived from the Florida Area Coastal Environment (FACE) water quality survey. Six distinct coastal locales were profiled – the Port Everglades and Hillsboro Inlets, Hollywood and Broward wastewater outfalls, and associated reef sites using culture-independent, high-throughput pyrosequencing of the 16S rRNA V4 region. More than 227,000 sequences helped describe longitudinal taxonomic …
Two Distinct Microbial Communities Revealed In The Sponge Cinachyrella, Marie L. Cuvelier, Emily Blake, Rebecca Mulheron, Peter J. Mccarthy, Patricia Blackwelder, Rebecca Vega-Thurber, Jose V. Lopez
Two Distinct Microbial Communities Revealed In The Sponge Cinachyrella, Marie L. Cuvelier, Emily Blake, Rebecca Mulheron, Peter J. Mccarthy, Patricia Blackwelder, Rebecca Vega-Thurber, Jose V. Lopez
Marine & Environmental Sciences Faculty Articles
Marine sponges are vital components of benthic and coral reef ecosystems, providing shelter and nutrition for many organisms. In addition, sponges act as an essential carbon and nutrient link between the pelagic and benthic environment by filtering large quantities of seawater. Many sponge species harbor a diverse microbial community (including Archaea, Bacteria and Eukaryotes), which can constitute up to 50% of the sponge biomass. Sponges of the genus Cinachyrella are common in Caribbean and Floridian reefs and their archaeal and bacterial microbiomes were explored here using 16S rRNA gene tag pyrosequencing. Cinachyrella specimens and seawater samples were collected from the …
Comparison Of Bacterial Diversity Within The Coral Reef Sponge, Axinella Corrugata, And The Encrusting Coral Erythropodium Caribaeorum, Jose V. Lopez, L. K. Ranzer, A. Ledger, B. Schoch, A. Duckworth, P. J. Mccarthy, R. G. Kerr
Comparison Of Bacterial Diversity Within The Coral Reef Sponge, Axinella Corrugata, And The Encrusting Coral Erythropodium Caribaeorum, Jose V. Lopez, L. K. Ranzer, A. Ledger, B. Schoch, A. Duckworth, P. J. Mccarthy, R. G. Kerr
Marine & Environmental Sciences Faculty Articles
We compared the Caribbean reef sponge, Axinella corrugata, with the Caribbean reef coral, Erythropodium caribaeorum for differences in their resident microbial communities. This cursory survey of bacterial diversity applied 16S rRNA gene sequences. Over 100 culture-independent sequences were generated from five different Axinella 16S rRNA libraries, and compared with 69 cultured isolates. The cultureindependent 16S rDNA clones displayed a higher diversity of Proteobacteria, including “uncultured” or “unknown” representatives from the Deltaproteobacteria. Arcobacterium, and Cyanobacteria were also found. We have also confirmed that Axinella sponges appeared to host specific microbial symbionts, similar to the previously identified clones termed “OSO” …
Molecular Techniques Reveal Wide Phyletic Diversity Of Heterotrophic Microbes Associated With Discodermia Spp. (Porifera: Demospongiae), Jose V. Lopez, Peter J. Mccarthy, Kathleen E. Janda, Robin Willoughby, Shirley A. Pomponi
Molecular Techniques Reveal Wide Phyletic Diversity Of Heterotrophic Microbes Associated With Discodermia Spp. (Porifera: Demospongiae), Jose V. Lopez, Peter J. Mccarthy, Kathleen E. Janda, Robin Willoughby, Shirley A. Pomponi
Marine & Environmental Sciences Faculty Articles
Sponges are well known to harbor large numbers of heterotrophic microbes within their mesohyl. Studies to determine the diversity of these associated microbes have been attempted for only a few shallow water species. We cultured various microorganisms from several species of Discodermia collected from deep water using the 'Johnson-Sea-Link' manned submersibles, and characterised them by standard microbiological identification methods. Characterisation of a small proportion (ca. 10%) of the total and potential eubacterial isolate collection with molecular systematics techniques revealed a wide diversity of microbes. Phylogenetic analyses of 32 small subunit (SSU) 16S-like rRNA gene sequences from different micorbes indicated high …