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Full-Text Articles in Marine Biology
A Dna Mini-Barcoding System For Authentication Of Processed Fish Products, Shadi Shokralla, Rosalee S. Hellberg, Sara M. Handy, Ian King, Mehrdad Hajibabaei
A Dna Mini-Barcoding System For Authentication Of Processed Fish Products, Shadi Shokralla, Rosalee S. Hellberg, Sara M. Handy, Ian King, Mehrdad Hajibabaei
Food Science Faculty Articles and Research
Species substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences—mini-barcodes—for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species commonly used in processed fish products in North America. Six mini-barcode primer pairs targeting short (127–314 bp) fragments of the cytochrome c oxidase I (CO1) DNA barcode region were developed by examining over 8,000 DNA barcodes from species in the U.S. Food and Drug Administration …