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Full-Text Articles in Marine Biology
Architectonica Karsteni Rutsch, 1934 (Gastropoda: Architectonicidae) In Seamounts Of The Nazca-Desventuradas Marine Park: First Evidence Of An Extant Population In Chilean Waters Since The Miocene, Cynthia M. Asorey, Javier Sellanes, Erin E. Easton, Rüdiger Bieler, Ariadna Mecho
Architectonica Karsteni Rutsch, 1934 (Gastropoda: Architectonicidae) In Seamounts Of The Nazca-Desventuradas Marine Park: First Evidence Of An Extant Population In Chilean Waters Since The Miocene, Cynthia M. Asorey, Javier Sellanes, Erin E. Easton, Rüdiger Bieler, Ariadna Mecho
School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations
The presence of Architectonicidae in Chile was previously recorded only from fossil material of eight species present during the Lower Miocene, when Architectonica karsteni had a geographic range that extended from Costa Rica to central Chile (34°S). No evidence of the presence of the family in Chile after the Lower Miocene have been reported. As part of this study, we report the discovery of A. karsteni at four seamountsat ~200 m water depth in the recently created Nazca-Desventuradas Marine Park, located ~900 km west of continental Chile. Morphological identification was based on protoconch diameter, coloration patterns, and teleoconch sculpture. We …
Characterization Of Bacterial Communities In Biscayne Bay Through Genomic Analysis, Eric Fortman
Characterization Of Bacterial Communities In Biscayne Bay Through Genomic Analysis, Eric Fortman
HCNSO Student Theses and Dissertations
Biscayne Bay is a shallow oligotrophic estuary in Southeast Florida. Channelization of rivers, and dredging of canals has greatly altered the historical flow of fresh water into the bay. This, coupled with the rise of a sprawling urban & suburban development, has greatly increased the nutrient load in the bay. This study examined the bacterial community at 14 stations throughout Biscayne Bay —6 stations were located at the mouths of canals; 1 upstream-canal station; 6 stations in the center of the bay; and one ocean influenced station, located near the entrance to the bay. One liter, surface water samples were …
Microbiome And Bacterioplankton Rrna Gene Sequence Data Collected From Gulf Of Mexico Seawater Samples, Cruises Dp03 And Dp04 From April - August 2016, Cole Easson, Lindsey Freed
Microbiome And Bacterioplankton Rrna Gene Sequence Data Collected From Gulf Of Mexico Seawater Samples, Cruises Dp03 And Dp04 From April - August 2016, Cole Easson, Lindsey Freed
DEEPEND Datasets
Seawater was collected and filtered for microbiome and bacterioplankton sequencing and analyses at various depths during planned DEEPEND cruise expeditions to the GOM in 2016. Filters were stored and then processed for total environmental genomic DNA according to standard methods (see earthmicrobiome.org). 16S rRNA amplicon libraries covering the V4 hypervariable regions were generated with universal PCR primers and then sequenced on an Illumina MiSeq DNA sequencing platform. Raw paired-end sequences were joined and quality filtered in the bioinformatics program, QIIME. Vertical baseline characterizations will track alpha and beta diversity at different depths ranging from 0 – 1500 m, assess seasonal …
Characterization Of The Marine Sponge Amphimedon Compressa Microbiome Across A Spatial Gradient, Renee Michelle Potens
Characterization Of The Marine Sponge Amphimedon Compressa Microbiome Across A Spatial Gradient, Renee Michelle Potens
HCNSO Student Theses and Dissertations
Diverse and ecologically important microbial communities (microbiomes) are symbiotic within marine sponges. In this study, the microbiome of Amphimedon compressa from three sample locations (Broward and Dade Counties, Southeast Florida, USA and the Southern Caribbean, Bocas del Toro, Panama) is characterized using 16S rRNA Illumina sequencing. The predominant taxa are Proteobacteria and Cyanobacteria, as expected for Low Microbial Abundance sponges, accounting for over 53% of the total microbiome community. The numbers of Operational Taxonomic Units (OTUs) decrease from Broward County (2,900) to Dade County (2,300) and then Bocas del Toro (1,200). The correlates to a decreasing north-south gradient of …
Dynamics Of Marine Bacterial Community Diversity Of The Coastal Waters Of The Reefs, Inlets, And Wastewater Outfalls Of Southeast Florida, Alexandra Mandina Campbell, Jay Fleisher, Christopher D. Sinigalliano, James R. White, Jose V. Lopez
Dynamics Of Marine Bacterial Community Diversity Of The Coastal Waters Of The Reefs, Inlets, And Wastewater Outfalls Of Southeast Florida, Alexandra Mandina Campbell, Jay Fleisher, Christopher D. Sinigalliano, James R. White, Jose V. Lopez
Marine & Environmental Sciences Faculty Articles
Coastal waters adjacent to populated southeast Florida possess different habitats (reefs, oceanic inlets, sewage outfalls) that may affect the composition of their inherent microbiomes. To determine variation according to site, season, and depth, over the course of 1 year, we characterized the bacterioplankton communities within 38 nearshore seawater samples derived from the Florida Area Coastal Environment (FACE) water quality survey. Six distinct coastal locales were profiled – the Port Everglades and Hillsboro Inlets, Hollywood and Broward wastewater outfalls, and associated reef sites using culture-independent, high-throughput pyrosequencing of the 16S rRNA V4 region. More than 227,000 sequences helped describe longitudinal taxonomic …
Comparison Of Bacterial Diversity Within The Coral Reef Sponge, Axinella Corrugata, And The Encrusting Coral Erythropodium Caribaeorum, Jose V. Lopez, L. K. Ranzer, A. Ledger, B. Schoch, A. Duckworth, P. J. Mccarthy, R. G. Kerr
Comparison Of Bacterial Diversity Within The Coral Reef Sponge, Axinella Corrugata, And The Encrusting Coral Erythropodium Caribaeorum, Jose V. Lopez, L. K. Ranzer, A. Ledger, B. Schoch, A. Duckworth, P. J. Mccarthy, R. G. Kerr
Marine & Environmental Sciences Faculty Articles
We compared the Caribbean reef sponge, Axinella corrugata, with the Caribbean reef coral, Erythropodium caribaeorum for differences in their resident microbial communities. This cursory survey of bacterial diversity applied 16S rRNA gene sequences. Over 100 culture-independent sequences were generated from five different Axinella 16S rRNA libraries, and compared with 69 cultured isolates. The cultureindependent 16S rDNA clones displayed a higher diversity of Proteobacteria, including “uncultured” or “unknown” representatives from the Deltaproteobacteria. Arcobacterium, and Cyanobacteria were also found. We have also confirmed that Axinella sponges appeared to host specific microbial symbionts, similar to the previously identified clones termed “OSO” …
Molecular Techniques Reveal Wide Phyletic Diversity Of Heterotrophic Microbes Associated With Discodermia Spp. (Porifera: Demospongiae), Jose V. Lopez, Peter J. Mccarthy, Kathleen E. Janda, Robin Willoughby, Shirley A. Pomponi
Molecular Techniques Reveal Wide Phyletic Diversity Of Heterotrophic Microbes Associated With Discodermia Spp. (Porifera: Demospongiae), Jose V. Lopez, Peter J. Mccarthy, Kathleen E. Janda, Robin Willoughby, Shirley A. Pomponi
Marine & Environmental Sciences Faculty Articles
Sponges are well known to harbor large numbers of heterotrophic microbes within their mesohyl. Studies to determine the diversity of these associated microbes have been attempted for only a few shallow water species. We cultured various microorganisms from several species of Discodermia collected from deep water using the 'Johnson-Sea-Link' manned submersibles, and characterised them by standard microbiological identification methods. Characterisation of a small proportion (ca. 10%) of the total and potential eubacterial isolate collection with molecular systematics techniques revealed a wide diversity of microbes. Phylogenetic analyses of 32 small subunit (SSU) 16S-like rRNA gene sequences from different micorbes indicated high …