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Full-Text Articles in Marine Biology
Application Of A Bioenergetics Framework For Assessing Sub-Lethal Effects Of Pollutants In The Freshwater Mussel Elliptio Complanata, Christopher G. Goodchild
Application Of A Bioenergetics Framework For Assessing Sub-Lethal Effects Of Pollutants In The Freshwater Mussel Elliptio Complanata, Christopher G. Goodchild
All Theses And Dissertations
Although biomarkers are frequently used to assess sublethal effects of contaminants, a lack of mechanistic linkages to higher-level effects limits the predictive power of biomarkers. Bioenergetics has been proposed as a framework for linking cellular effects to whole-animal effects. We investigated sublethal effects of exposure to wastewater treatment facility effluent in freshwater mussels in situ, thereby capturing ecologically relevant exposure conditions. Our study focused on the energetic biomarker AMP-activated protein kinase (AMPK), while also considering more traditional biomarkers like heat shock proteins (HSP70), and antioxidant enzymes (i.e., superoxide dismutase (SOD), glutathione-S-transferase (GST)). We examined biomarkers at mRNA and protein levels. …
Differential Response Of Amp Activated Protein Kinase (Ampk) And Hsp70 To Temperature Stress In The Gastropod, Nucella Lapillus, Emily Zimmermann
Differential Response Of Amp Activated Protein Kinase (Ampk) And Hsp70 To Temperature Stress In The Gastropod, Nucella Lapillus, Emily Zimmermann
All Theses And Dissertations
Populations of the gastropod Nucella lapillus are polymorphic for shell color, with light-colored shells predominating on warmer, wave-protected shores and dark-colored shells limited primarily to cooler, wave-exposed shores. During thermal stress, darker shells attain higher body temperatures than lighter shells. These results suggest that heat stress may determine field distribution patterns. However, there is currently little evidence of physiological consequences of thermal stress in these organisms. Following the guiding hypothesis that heat stress leads to cellular energy depletion, we explored whether the central energy regulator AMP-activated Protein Kinase (AMPK) is activated by heat stress. We compared this response in both …