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Full-Text Articles in Marine Biology
Phosphorus Cycling In The Sargasso Sea: Investigation Using The Oxygen Isotopic Composition Of Phosphate, Enzyme-Labeled Fluorescence, And Turnover Times, Karen Mclaughlin, Jill A. Sohm, Gregory A. Cutter, Michael W. Lomas, Adina Paytan
Phosphorus Cycling In The Sargasso Sea: Investigation Using The Oxygen Isotopic Composition Of Phosphate, Enzyme-Labeled Fluorescence, And Turnover Times, Karen Mclaughlin, Jill A. Sohm, Gregory A. Cutter, Michael W. Lomas, Adina Paytan
OES Faculty Publications
Dissolved inorganic phosphorus (DIP) concentrations in surface water of vast areas of the ocean are extremely low (<10 nM) and phosphorus (P) availability could limit primary productivity in these regions. We explore the use of oxygen isotopic signature of dissolved phosphate (δ18OPO4) to investigate biogeochemical cycling of P in the Sargasso Sea, Atlantic Ocean. Additional techniques for studying P dynamics including 33P-based DIP turnover time estimates and percent of cells expressing alkaline phosphatase (AP) activity as measured by enzyme-labeling fluorescence are also used. In surface waters, δ18OPO4 values were lower than equilibrium by 3–6%, indicative of dissolved organic phosphorous (DOP) remineralization by extracellular enzymes. An isotope mass balance model using a variety of possible combinations of …
Benthic Microalgal Production At Stellwagen Bank, Massachusetts Bay, Usa, Lawrence B. Cahoon, Guy R. Beretich Jr., Carrie J. Thomas, Amy M. Mcdonald
Benthic Microalgal Production At Stellwagen Bank, Massachusetts Bay, Usa, Lawrence B. Cahoon, Guy R. Beretich Jr., Carrie J. Thomas, Amy M. Mcdonald
OES Faculty Publications
Benthic microalgal chlorophyll a and production were measured at 3 sites at Stellwagen Bank, a cold temperate continental shelf habitat in Massachusetts Bay, USA, during August 1991. Benthic microalgal chlorophyll a averaged 39.8 Mg M-2, vs average integrated phytoplankton chlorophyll a of 25.9 mg m-2. Gross benthic microalgal production, measured by oxygen exchange in clear and opaque benthic chambers, averaged 20.9 mg C m-2 h-1. This production was supported by average daily light fluxes to the bottom that never exceeded 1 % of surface incident radiation and were as low as 4.7 μE …
Development And Evaluation Of Tracer Particles For Use In Microzooplankton Herbivory Studies, Mary Putt
Development And Evaluation Of Tracer Particles For Use In Microzooplankton Herbivory Studies, Mary Putt
OES Faculty Publications
Two methods of preparing algae for use as tracer particles in single species measurements of microzooplankton herbivory were evaluated. Algae were either heat-killed and labelled with 5-(4,6-dichlorotriazin-2-yl amino fluorescein) (DTAF) (Rublee & Gallegos 1989; Mar, Ecol. Prog. Ser. 51: 221-227) or stained with hydroethidine (HYD). Both DTAF and HYD-stained algae were readily visible within the digestive vacuoles of most microzooplankton collected in estuarine and coastal waters of Massachusetts (USA) and preserved with Lugol's iodine. However, DTAF was ineffective at staining several chromophytic algae and the heat-kill process reduced cell volume by ≥ 50% in several of the algae which were …