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Full-Text Articles in Marine Biology
Salty Sensors, Fresh Ideas: The Use Of Molecular And Imaging Sensors In Understanding Plankton Dynamics Across Marine And Freshwater Ecosystems, Trisha Lyn Spanbauer, Christian Briseno-Avena, Kathleen Johnson Pitz, Elizabeth A. Suter
Salty Sensors, Fresh Ideas: The Use Of Molecular And Imaging Sensors In Understanding Plankton Dynamics Across Marine And Freshwater Ecosystems, Trisha Lyn Spanbauer, Christian Briseno-Avena, Kathleen Johnson Pitz, Elizabeth A. Suter
Faculty Works: Biology, Chemistry, and Environmental Studies
Understanding plankton dynamics in marine ecosystems has been advanced using in situ molecular and imaging instrumentation. A range of research objectives have been addressed through high‐resolution autonomous sampling, from food web characterization to harmful algal bloom dynamics. When used together, molecular and imaging sensors can cover the full‐size range, genetic identity, and life stages of plankton. Here, we briefly review a selection of in situ instrumentation developed for the collection of molecular and imaging information on plankton communities in marine ecosystems. In addition, we interviewed a selection of instrumentation developers to determine if the transfer of sensor technology from marine …
Single-Cell Growth Rates In Photoautotrophic Populations Measured By Stable Isotope Probing And Resonance Raman Microspectrometry, Gordon T. Taylor, Elizabeth A. Suter, Zhuo Q. Li, Stephanie Chow, Dallyce Stinton, Tatiana Zaliznyak, Steven R. Beaupre
Single-Cell Growth Rates In Photoautotrophic Populations Measured By Stable Isotope Probing And Resonance Raman Microspectrometry, Gordon T. Taylor, Elizabeth A. Suter, Zhuo Q. Li, Stephanie Chow, Dallyce Stinton, Tatiana Zaliznyak, Steven R. Beaupre
Faculty Works: Biology, Chemistry, and Environmental Studies
A newmethod tomeasure growth rates of individual photoautotrophic cells by combining stable isotope probing (SIP) and single-cell resonance Raman microspectrometry is introduced. This report explores optimal experimental design and the theoretical underpinnings for quantitative responses of Raman spectra to cellular isotopic composition. Resonance Raman spectra of isogenic cultures of the cyanobacterium, Synechococcus sp., grown in 13C-bicarbonate revealed linear covariance between wavenumber (cm−1) shifts in dominant carotenoid Raman peaks and a broad range of cellular 13C fractional isotopic abundance. Single-cell growth rates were calculated from spectra-derived isotopic content and empirical relationships. Growth rates among any 25 cells in a sample varied …
Particle Association Of Enterococcus And Total Bacteria In The Lower Hudson River Estuary, Usa, Elizabeth A. Suter, Andrew R. Juhl, Gegory D. O'Mullan
Particle Association Of Enterococcus And Total Bacteria In The Lower Hudson River Estuary, Usa, Elizabeth A. Suter, Andrew R. Juhl, Gegory D. O'Mullan
Faculty Works: Biology, Chemistry, and Environmental Studies
Bacterial particle association has important consequences for water-quality monitoring and modeling. Parti-cle association can change vertical and horizontal transport of bacterial cells, as well as patterns of persis-tence and production. In this study, the abundance and particle association of total bacteria and the fe-cal-indicator, Enterococcus, were quantified between June and October 2008 in the lower Hudson River Es-tuary (HRE). Twelve sites were sampled, including mid-channel, near shore, and tributary habitats, plus a sewage outfall. Total bacterial cell counts averaged 9.2 × 109 ± 6.4 × 109 cell·l–1 (1 standard deviation), com-parable to previous sampling in the HRE. Unlike earlier studies, …