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Perkinsus marinus; protozoan; in vitro; protein-free chemically defined culture medium; oyster parasite; Crassostrea virginica
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Development Of A Protein-Free Chemically Defined Culture Medium For The Propagation Of The Oyster Pathogen Perkinsus Marinus, Jf Lapeyre, M Faisal
Development Of A Protein-Free Chemically Defined Culture Medium For The Propagation Of The Oyster Pathogen Perkinsus Marinus, Jf Lapeyre, M Faisal
VIMS Articles
In the present study we describe a protein-free, chemically defined culture medium, designated JL-ODRP-3, which supports the propagation of Perkinsus marinus, a parasite of the eastern oyster, Crassotrea virginica. P. marinus adapted rapidly to the defined medium and the growth rate of the protozoan increased significantly following a few subcultures. Two isolates of P. marinus, one from the Chesapeake Bay (Virginia) and the other from the Gulf of Mexico (Texas) were cultured for at least ten passes. The doubling times far the isolates from Virginia and Texas, in Jog phase, were 18 +/- 1.2 and 28.6 +/- 3.2 hours respectively, …