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Evidence For Dna Methylation-Sensitive Transactivator Mediated Regulation Of The Expression Of An Epstein-Barr Virus Neutralizing Antigen, Sridhar Chalasani
Evidence For Dna Methylation-Sensitive Transactivator Mediated Regulation Of The Expression Of An Epstein-Barr Virus Neutralizing Antigen, Sridhar Chalasani
Masters Theses
DNA methylation has a profound effect on the expression of a number of eukaryotic genes. This study demonstrates that an Epstein-Barr virus (EBV) positive Burkitt's lymphoma cell line, Raji, contains the gene encoding gp350/220 but is not expressed. Frog virus 3 (FV3), an iridovirus, has the ability to overcome the inhibitory effect of DNA methylation. Fusion of FV3-infected cells with Raji cells resulted in expression of gp350/220, suggesting that gp350/220 is regulated by DNA methylation. Further evidence for this hypothesis was obtained by transfecting B95.8 cells with HpaII/HhaI methylated plasmid pLATE-CAT, containing the gp350/220 promoter. Southern blot analyses and a …