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Immunology and Infectious Disease Commons™
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Full-Text Articles in Immunology and Infectious Disease
Tetraspanins As Potential Therapeutic Candidates For Targeting Flaviviruses, Waqas Ahmed, Girish Neelakanta, Hameeda Sultana
Tetraspanins As Potential Therapeutic Candidates For Targeting Flaviviruses, Waqas Ahmed, Girish Neelakanta, Hameeda Sultana
Biological Sciences Faculty Publications
Tetraspanin family of proteins participates in numerous fundamental signaling pathways involved in viral transmission, virus-specific immunity, and virus-mediated vesicular trafficking. Studies in the identification of novel therapeutic candidates and strategies to target West Nile virus, dengue and Zika viruses are highly warranted due to the failure in development of vaccines. Recent evidences have shown that the widely distributed tetraspanin proteins may provide a platform for the development of novel therapeutic approaches. In this review, we discuss the diversified and important functions of tetraspanins in exosome/extracellular vesicle biology, virus-host interactions, virus-mediated vesicular trafficking, modulation of immune mechanism(s), and their possible role(s) …
Tick-Borne Flavivirus Inhibits Sphingomyelinase (Issmase), A Venomous Spider Ortholog To Increase Sphingomyelin Lipid Levels For Its Survival In Ixodes Scapularis Ticks, Pravesh Regmi, Supreet Khanal, Girish Neelakanta, Hameda Sultana
Tick-Borne Flavivirus Inhibits Sphingomyelinase (Issmase), A Venomous Spider Ortholog To Increase Sphingomyelin Lipid Levels For Its Survival In Ixodes Scapularis Ticks, Pravesh Regmi, Supreet Khanal, Girish Neelakanta, Hameda Sultana
Biological Sciences Faculty Publications
Our previous study showed that cells from medically important arthropods, such as ticks, secrete extracellular vesicles (EVs) including exosomes that mediate transmission of flavivirus RNA and proteins to the human cells. Understanding the molecular determinants and mechanism(s) of arthropod-borne flavivirus transmission via exosome biogenesis is very important. In this current study, we showed that in the presence of tick-borne Langat Virus (LGTV; a member of tick-borne encephalitis virus complex), the expression of arthropod IsSMase, a sphingomyelinase D (SMase D) that catalyzes the hydrolytic cleavage of substrates like sphingomyelin (SM) lipids, was significantly reduced in both Ixodes scapularis ticks (in vivo) …
Discovery Of Exosomes From Tick Saliva And Salivary Glands Reveals Therapeutic Roles For Cxcl12 And Il-8 In Wound Healing At The Tick-Human Skin Interface, Wenshuo Zhou, Faizan Tahir, Joseph Che-Yen Wang, Michael Woodson, Michael B. Sherman, Shahid Karim, Girish Neelakanta, Hameeda Sultana
Discovery Of Exosomes From Tick Saliva And Salivary Glands Reveals Therapeutic Roles For Cxcl12 And Il-8 In Wound Healing At The Tick-Human Skin Interface, Wenshuo Zhou, Faizan Tahir, Joseph Che-Yen Wang, Michael Woodson, Michael B. Sherman, Shahid Karim, Girish Neelakanta, Hameeda Sultana
Biological Sciences Faculty Publications
Ticks secrete various anti-coagulatory, anti-vasoconstrictory, anti-inflammatory, and anti-platelet aggregation factors in their saliva at the bite site during feeding to evade host immunological surveillance and responses. For the first time, we report successful isolation of exosomes (small membrane-bound extracellular signaling vesicles) from saliva and salivary glands of partially fed or unfed ixodid ticks. Our data showed a novel role of these in vivo exosomes in the inhibition of wound healing via downregulation of C-X-C motif chemokine ligand 12 (CXCL12) and upregulation of interleukin-8 (IL-8). Cryo-electron microscopy (cryo-EM) analysis revealed that tick saliva and salivary glands are composed of heterogeneous populations …