Immunology and Infectious Disease Commons^™

Kinetic Analysis And Epitope Mapping Of Monoclonal Antibodies To Salmonella Typhimurium Flagellin Using A Surface Plasmon Resonance Biosensor, Devendra Bhandari, Fur-Chi Chen, Shreya Hamal, Roger C. Bridgman

Human Sciences Faculty Research

Salmonella Typhimurium is one of the leading causes of foodborne diseases worldwide. Biosensors and immunoassays utilizing monoclonal antibodies are widely used for the detection and subtyping of S. Typhimurium. However, due to insufficient information on the nature of binding with S. Typhimurium flagellin, the selection of appropriate antibodies for assay development is a cumbersome task. Hence, we aimed to compare the binding kinetics of a panel of monoclonal antibodies and their relative binding sites to flagellin antigen using a surface plasmon resonance biosensor. Initially, the flagellin was captured on the sensor surface through an immobilized anti-flagellin antibody. The interactions of …