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Full-Text Articles in Immunology and Infectious Disease
Sequence And The Developmental And Tissue-Specific Regulation Of The First Complete Vitellogenin Message From Ticks, Deborah M. Thompson, Sayed M.S. Khalil, Laura A. Jeffers, Daniel E. Sonenshine, Robert D. Mitchell, Christopher J. Osgood, R. Michael Roe
Sequence And The Developmental And Tissue-Specific Regulation Of The First Complete Vitellogenin Message From Ticks, Deborah M. Thompson, Sayed M.S. Khalil, Laura A. Jeffers, Daniel E. Sonenshine, Robert D. Mitchell, Christopher J. Osgood, R. Michael Roe
Biological Sciences Faculty Publications
The first full-length mRNA for vitellogenin (Vg) from ticks was sequenced. This also represents the first complete sequence of Vg from the Chelicerata and of a heme binding Vg. The Vg cDNA from the American dog tick, Dermacentor variabilis was 5744nt in length (GenBank Accession number AY885250), which coded for a protein of 1843aa with a calculated molecular weight of 208kD. This protein had an 18 aa signal sequence, a single RXXR cleavage signal that would generate two subunits (49.5 and 157K in molecular weight) and lipoprotein N-terminal and carboxy von Willebrand factor type D domains. Tryptic digest MS analysis …
Design, Synthesis, And Evaluation Of 10-N-Substituted Acridones As Novel Chemosensitizers In Plasmodium Falciparum, Jane X. Kelly, Martin J. Smilkstein, Roland A. Cooper, Kristin D. Lane, Robert A. Johnson, Aaron Janowsky, Rozalia A. Dodean, David J. Hinrichs, Rolf Winter, Michael Riscoe
Design, Synthesis, And Evaluation Of 10-N-Substituted Acridones As Novel Chemosensitizers In Plasmodium Falciparum, Jane X. Kelly, Martin J. Smilkstein, Roland A. Cooper, Kristin D. Lane, Robert A. Johnson, Aaron Janowsky, Rozalia A. Dodean, David J. Hinrichs, Rolf Winter, Michael Riscoe
Biological Sciences Faculty Publications
A series of novel 10-N-substituted acridones, bearing alkyl side chains with tertiary amine groups at the terminal position, were designed, synthesized, and evaluated for the ability to enhance the potency of quinoline drugs against multidrug-resistant (MDR) Plasmodium falciparum malaria parasites. A number of acridone derivatives, with side chains bridged three or more carbon atoms apart between the ring nitrogen and terminal nitrogen, demonstrated chloroquine (CQ)-chemosensitizing activity against the MDR strain of P. falciparum (Dd2). Isobolograrn analysis revealed that selected candidates demonstrated significant synergy with CQ in the CQ-resistant (CQR) parasite Dd2 but only additive (or indifferent) interaction in the CQ-sensitive …
Tissue And Life Stage Distribution Of A Defensin-Like Gene In The Lone Star Tick Amblyomma Americanum, S. Michelle Todd, Daniel E. Sonenshine, Wayne L. Hynes
Tissue And Life Stage Distribution Of A Defensin-Like Gene In The Lone Star Tick Amblyomma Americanum, S. Michelle Todd, Daniel E. Sonenshine, Wayne L. Hynes
Biological Sciences Faculty Publications
The transcript sequence of the Amblyomma americanum Linnaeus (Acari: Ixodidae) defensin, termed amercin (amn), was ascertained and a 219-bp amn coding region identified. The gene encodes a 72-amino acid prepropeptide with a putative 37-amino acid mature peptide. This gene shows little similarity to either of the defensins from Amblyomma hebraeum Koch, the only other Amblyomma species for which a defensin has been described. Sequence comparisons with other tick defensins reveal amn to be shorter (6 bp or 2 amino acids) than the Ixodes scapularis Linnaeus (Acari: Ixodidae) and Dermacentor variabilis (Say) (Acari: Ixodidae) defensin sequences. The amercin prepropeptide …