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Immunology and Infectious Disease Commons™
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Full-Text Articles in Immunology and Infectious Disease
Characterization Of Induced Rnai Silencing Of Vaccinia Virus Essential Genes, Kewa Jiang
Characterization Of Induced Rnai Silencing Of Vaccinia Virus Essential Genes, Kewa Jiang
University Scholar Projects
Vaccinia virus (VACV) is a large double-stranded DNA virus and the prototypical member of the family Poxviridae and is most notable for its use as the vaccine that eradicated smallpox (variola virus). More recently, VACV has been used to develop recombinant vaccines and immunotherapies. However, many of these processes require VACV replication to be tightly controlled. RNA interference (RNAi) is a powerful tool for in vitro silencing of mRNAs that are complimentary to 19-21 base pairs (bp) of double-stranded RNA (dsRNA). This project outlines the design and preliminary analysis of two inducible RNAi silencing constructs targeting multiple VACV essential genes …
Optimization Of Expression And Purification Of The Rig-I-Like-Receptor, Lgp2, Srinath-Reddi Pingle
Optimization Of Expression And Purification Of The Rig-I-Like-Receptor, Lgp2, Srinath-Reddi Pingle
University Scholar Projects
The innate immune system is one of the first lines of defense against pathogens. RIG-I like receptors (RLRs) are a class of cytosolic receptors that recognize molecular patterns of invading pathogens and signal for downstream interferon induction. The RLR family consists of three proteins, RIG-I, MDA5, and LGP2. MDA5 and LGP2 work together to recognize and bind long viral dsRNA. LGP2 is thought to regulate MDA5 activation, but little is understood about this process. Characterization of the mechanism of action of these receptors requires careful biophysical and biochemical analyses. I have developed expression and purification of methods LGP2 and MDA5 …
Characterization Of Induced Rnai Silencing Of Vaccinia Virus Essential Genes, Kewa Jiang
Characterization Of Induced Rnai Silencing Of Vaccinia Virus Essential Genes, Kewa Jiang
Honors Scholar Theses
Vaccinia virus (VACV) is a large double-stranded DNA virus and the prototypical member of the family Poxviridae and is most notable for its use as the vaccine that eradicated smallpox (variola virus). More recently, VACV has been used to develop recombinant vaccines and immunotherapies. However, many of these processes require VACV replication to be tightly controlled. RNA interference (RNAi) is a powerful tool for in vitro silencing of mRNAs that are complementary to 19-21 base pairs (bp) of double-stranded RNA (dsRNA). This project outlines the design and preliminary analysis of two inducible RNAi silencing constructs targeting multiple VACV essential genes …