Immunology and Infectious Disease Commons^™

Comparison Of The Humoral Immune Response Following Both Bacterial Challenge And Rnai Of Major Factors On Proliferation Of Bartonella Quintana In The Human Louse, Jake Zina

Masters Theses

Human body lice, Pediculus humanus humanus, and head lice, Pediculus humanus capitis, have been hematophagous ectoparasites of humans for thousands of years. Despite being ecotypes, only body lice are known to transmit bacterial diseases to humans, and it appears that lower humoral and cellular immune responses allow body lice to possess a higher vector competence. We previously observed that the transcription level of the defensin 1 gene was up-regulated only in head lice following oral challenge of Bartonella quintana, a causative agent of trench fever, and also that body lice excreted more viable B. quintana in their …

Assessment Of The Immune Response To Posthodiplostomum Minimum Infection In Bluegills, Olamide S. Olayinka

Masters Theses

Bluegills (Lepomis macrochirus) are common intermediate hosts for white grub (Posthodiplostomum minimum). They tolerate heavy infections with minimal effect on condition and continue to accumulate metacercariae as they age. This suggests that any immune response to this parasite might not be effective. This study was conducted to better understand the immune mechanisms underlying P. minimum infection in bluegills.

Infected organs (liver, kidney, and heart) were examined histologically, and serum from infected fish was tested for antibodies to white grub. Juvenile flukes were recovered from isolated metacercarial cysts. Polyclonal antibodies were produced in mice against white grub …

Mathematical Modeling Of T Cell Clustering Following Malaria Infection In Mice, Reka Katalin Kelemen

Masters Theses

Malaria is the result of the immune system's unsuccessful clearance of hepatocytes (liver cells) infected by the eukaryotic pathogen of the Plasmodium genus. It has been shown that CD8 T cells are required and sufficient for protective immunity against malaria in mice [29, 36], but the mechanisms by which they find and eliminate infected hepatocytes are not known yet. Recently we reported the formation of CD8 T cell clusters consisting of up to 25 cells around infected cells [8]. Our mathematical modeling and data analysis revealed that malaria-specific T cells likely recruit each other and also non-malaria-specific T cells to …

Plasmodium Suppresses T Cell Responses To Heterologous Infections By Impairing T Cell Activation, Chelsi Elizabeth White

Masters Theses

Malaria is a devastating disease caused by parasites of the genus Plasmodium. Plasmodium falciparum, which is responsible for most malaria related fatalities, suppresses host immune responses during heterologous coinfections or following vaccination. However the mechanisms responsible for this defect are not well defined. The mechanism and to what extent this immunosuppression is occurring was investigated. This study demonstrates that both dendritic cell and T cell activation are impaired following a Plasmodium infection, ultimately altering the adaptive T cell response to secondary infections. T cell suppression is evident early on following a secondary infection and continues throughout the peak of parasitemia. …

Impacts Of Predation Risk And Development On Susceptibility Of North American Anurans To Ranaviruses, Nathan Alden Haislip

Masters Theses

For over three decades, amphibian populations have been declining across the globe. Emerging infectious diseases are responsible for some of these declines. Ranaviruses have caused die-offs in wild amphibian populations on 4 continents, in 5 Canadian provinces, and in over 25 U.S. states. In order to understand host-pathogen dynamics, it is critical to establish baseline information on species susceptibility and the effects of natural stressors. The goal of my thesis research was to quantify the effects of anuran development and exposure to invertebrate predators on species-specific susceptibility to ranavirus. My experiments were designed in factorial arrangements, and consisted of exposure …