Immunology and Infectious Disease Commons^™

Enhancement Of Atp Levels And Glucose Metabolism During An Infection By Chlamydia: Nmr Studies Of Living Cells, David M. Ojcius, H. Degani, Joel Mispelter, Alice Dautry-Varsat

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The Chlamydia species are obligate intracellular bacteria that proliferate only within the infected cell. Since the extracellular bacteria are metabolically inert and there are no cell-free systems for characterizingChlamydia metabolism, we studied metabolic changes related to ATP synthesis and glycolysis in HeLa cells infected withChlamydia psittaci during the course of the 2-day infection cycle using noninvasive 31P and 13C NMR methods. We find that the infection stimulates ATP synthesis in the infected cell, with a peak of ATP levels occurring midway through the infection cycle, when most of the metabolically active bacteria are proliferating. The infection also stimulates synthesis of …