Genetics and Genomics Commons^™

A Radiation Hybrid Map Of The Cat Genome: Implications For Comparative Mapping, William J. Murphy, Shan Sun, Zhang-Qun Chen, Naoya Yuhki, Deborah Hirschmann, Marilyn Menotti-Raymond, Stephen J. O'Brien

Biology Faculty Articles

Ordered gene maps of mammalian species are becoming increasingly valued in assigning gene variants to function in human and animal models, as well as recapitulating the natural history of genome organization. To extend this power to the domestic cat, a radiation hybrid (RH) map of the cat was constructed integrating 424 Type I-coding genes with 176 microsatellite markers, providing coverage over all 20 feline chromosomes. Alignment of parallel RH maps of human and cat reveal 100 conserved segments ordered (CSOs) between the species, nearly three times the number observed with reciprocal chromosome painting analyses. The observed number is equivalent to …

New World Nine-Primaried Oscine Relationships: Constructing A Molecular Framework, John Klicka, Kevin P. Johnson, Scott M. Lanyon

Ornithology Program (HRC)

Historically, a paucity of comparative morphological characters has led to much debate regarding relationships within and among the major lineages of New World nine-primaried oscines. More recently, DNA-DNA hybridization studies have provided novel and testable hypotheses of relationships, although no consensus has been reached. For 40 songbird taxa, we obtained 1,929 base pairs (bp) of DNA sequence from the mitochondrial cytochrome-b (894 bp) and NADH dehydrogenase subunit 2 (1,035 bp) genes. Phylogenetic analyses confirm the monophyly of this assemblage as traditionally defined. The lineages delineated historically on morphological grounds are retained; finches (Fringillinae) are sister to a well-supported clade …

Rapid Communication: Porcine Vitamin D-25-Hydroxylase Maps To Chromosome 5, Daniel C. Ciobanu, R. K. Raven, M. F. Rothschild, N. H. Bell

Department of Animal Science: Faculty Publications

Locus Name. Vitamin D-25-hydroxylase.

Species. Sus scrofa.

Locus Symbol. CYP25.

Source of Primers. PCR primers were designed from the published sequence for porcine vitamin D-25-hydroxylase (CYP25) cDNA (GenBank accession no. Y16417). The position of the forward and reverse primers corresponds to 506 to 530 bp and 830 to 854 bp, respectively, of the cDNA. The primer pair amplified a 348-bp fragment of the cDNA and an 862-bp fragment from the pig genomic DNA. The 862-bp fragment (Gen- Bank accession no. AF284378) was subcloned into a pNoTA vector and identity was confirmed by sequencing. Genomic sequences from 1 to 74, …

Comparison Of Plasma Fsh Concentration In Boars And Gilts From Lines Selected For Ovulation Rate And Embryonal Survival, And Litter Size And Estimation Of (Co)Variance Components For Fsh And Ovulation Rate, J. D. Cassady, Rodger K. Dennison, J. J. Ford

Department of Animal Science: Faculty Publications

The objective of this research was to determine whether plasma concentration of FSH was genetically correlated with ovulation rate and thus was a useful trait for indirect selection. Blood samples were collected from 619 animals from five lines of pigs. Line I was selected for increased index of ovulation rate and embryonal survival, and Line C was its randomly selected control. Pigs sampled from Lines I and C were from generations 12 and 13. Pigs from three additional lines that were derived from eighth-generation pigs of Lines I and C also were used. These lines were Line C2, a randomly …