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Full-Text Articles in Genetics and Genomics

Actin Gene Family Evolution And The Phylogeny Of Coleoid Cephalopods (Mollusca: Cephalopoda), David B. Carlini, Kimberly S. Reece, John Graves Aug 2000

Actin Gene Family Evolution And The Phylogeny Of Coleoid Cephalopods (Mollusca: Cephalopoda), David B. Carlini, Kimberly S. Reece, John Graves

VIMS Articles

Phylogenetic analysis conducted on a 784-bp fragment of 82 actin gene sequences of 44 coleoid cephalopod taxa, along with results obtained from genomic Southern blot analysis, confirmed the presence of at least three distinct actin loci in coleoids. Actin isoforms were characteri zed through phylogenetic analysis of representative cephalopod sequences from each of the three isoforms, along with translated actin cDNA sequences from a diverse array of metazoan taxa downloaded from GenBank. One of the three isoforms found in cephalopods was closely related to actin sequences expressed in the muscular tissues of other molluscs. A second isoform was most similar …


Application Of Genetic Markers To Provide Species Identification And Define Stock Structure: Analyses Of Selected Marine Fishes Of The Mid -Atlantic Bight, Jan F. Cordes Jan 2000

Application Of Genetic Markers To Provide Species Identification And Define Stock Structure: Analyses Of Selected Marine Fishes Of The Mid -Atlantic Bight, Jan F. Cordes

Dissertations, Theses, and Masters Projects

Molecular markers and techniques were employed to develop a genetic key for the forensic identification of 16 species of Chesapeake Bay sportfishes and to investigate the stock structure of one of those species, the weakfish Cynoscion regalis. Regions within the ATP synthetase 6 (ATPase 6), Cytochrome b , cytochrome c oxidase I, NADH dehydrogenase 4 (ND4), and 12S/16S ribosomal RNA mitochondrial genes were amplified using the polymerase chain reaction (PCR) and digested with a bank of restriction endonucleases to find a genetic marker that exhibited complete interspecific differentiation and low intraspecific variation. Complete separation of all sixteen species was accomplished …


A Molecular Phylogeny Of The Sparidae (Perciformes: Percoidei), Thomas M. Orrell Jan 2000

A Molecular Phylogeny Of The Sparidae (Perciformes: Percoidei), Thomas M. Orrell

Dissertations, Theses, and Masters Projects

The Sparidae are a diverse group of over 110 marine species whose putative six subfamilies have been defined primarily on the basis of dentition and feeding type. Monophyly of the subfamilies has not been tested, nor have the phylogenetic relationships of all sparid genera been hypothesized. I used mitochondrial DNA sequences from the complete cytochrome b gene (1140 bp) and the partial 16S gene (621 bp) in independent and combined analyses to test the monophyly of the Sparidae, elucidate the inter-relationships of the 33 recognized genera of the Sparidae, test the validity of the six subfamilies of the Sparidae, and …