Genetics and Genomics Commons^™

Variation At Tri- And Tetranucleotide Repeat Microsatellite Loci In The Fruit Bat Genus Cynopterus (Chiroptera: Pteropodidae), Jay F. Storz

Jay F. Storz Publications

There is considerable uncertainty surrounding the taxonomic relationship between Cynopterus sphinx and C. brachyotis, and the status of the many named forms within C. sphinx (Storz & Kunz 1999). Polymorphic microsatellite markers for cynopterine fruit bats would greatly aid efforts to elucidate species boundaries and genetic correlates of morphological variation within species. To assess levels of variation in C. sphinx and C. brachyotis, microsatellite genotypes were obtained for a total of 731 bats (620 C. sphinx and 111 C. brachyotis).

Social Structure Of A Polygynous Tent-Making Bat, Cynopterus Sphinx (Megachiroptera), Jay F. Storz, Hari Bhat, Thomas H. Kunz

Jay F. Storz Publications

The social structure of an Old World tent-making bat Cynopterus sphinx (Megachiroptera), was investigated in western India. A combination of census and mark–recapture data over 2 years (1996–98) was used to infer the form of the mating system, compositional stability of social groups and mode of new social group formation. The breeding population of C. sphinx was subdivided into diurnal roosting colonies, each of which contained one to five discrete roosting groups and often one or more solitary bats in adjacent roosts. Bats most frequently roosted in stem tents constructed in the flower/fruit clusters of the kitul palm Caryota urens …

"An Evolutionary No Man’S Land" And "Reply From L. G. Harshman And A. A. Hoffmann", Margarida Matos, André Levy, Henrique Teotónio, Michael R. Rose, Lawrence G. Harshman, Ary A. Hoffmann

Lawrence G. Harshman Publications

The gap between evolutionary studies in laboratory versus natural populations is a persistent problem.

Dispersion And Site Fidelity In A Tent-Roosting Population Of The Short-Nosed Fruit Bat (Cynopterus Sphinx) In Southern India, Jay F. Storz, J. Balasingh, P. Thiruchenthil Nathan, K. Emmanuel, Thomas H. Kunz

Jay F. Storz Publications

Patterns of dispersion and site fidelity were investigated in a tent-roosting population of the short-nosed fruit bat, Cynopterus sphinx (Megachiroptera), in southern India. A local population of C. sphinx occupied diurnal roosts in a variable subset of 45 stem tents constructed within the dense foliage of mast trees (Polyalthia longifolia). Individually marked tent-roosting bats were visually censused over the course of a 38-d interval spanning the postpartum oestrus period. On any given day, 33.3-85.7% (mean = 60.8%, SD = 14.2) of adult males roosted singly, with the remainder holding harems of 1-10 breeding females (mean = 3.01, SD …

Oxidative Stress Resistance: A Robust Correlated Response To Selection In Extended Longevity Lines Of Drosophila Melanogaster?, Lawrence G. Harshman, Beth A. Haberer

Lawrence G. Harshman Publications

Stress resistance is associated with longevity in Drosophila melanogaster and other model organisms used for genetic research. The present study tests for oxidative stress resistance in one set of lines selected for late-life reproduction and extended longevity. Both females and males from the selected lines were appreciably more resistant to oxidative stress than were flies from the control lines. A relative increase in oxidative stress resistance is a correlated response to selection in this laboratory selection experiment. Increased oxidative stress resistance appears to be a relatively robust correlated response to laboratory selection for late-life reproduction and extended longevity.

Identification Of The Transactivation Domain Of The Transcription Factor Sox-2 And An Associated Co-Activator, Tamara K. Nowling, Lance R. Johnson, Matthew S. Wiebe, Angie Rizzino

Nebraska Center for Virology: Faculty Publications

The importance of interactions between Sox and POU transcription factors in the regulation of gene expression is becoming increasingly apparent. Recently, many examples of the involvement of Sox-POU partnerships in transcription have been discovered, including a partnership between Sox-2 and Oct-3. Little is known about the mechanisms by which these factors modulate transcription. To better understand the molecular interactions involved, we mapped the location of the transactivation do-main of Sox-2. This was done in the context of its interaction with Oct-3, as well as its ability to transactivate as a fusion protein linked to the DNA-binding domain of Gal4. Both …

Recent Progress In Psychiatric Genetics—Some Hope But No Hype, Scott F. Stoltenberg, Margit Burmeister

Department of Psychology: Faculty Publications

The reputation of the field of psychiatric genetics has recently become tarnished in the view of many human geneticists. Too many linked loci were claimed and withdrawn, too many association studies published and not confirmed, and, more recently, too many new and different chromosomal regions have been implicated for the same disorder. Here, we summarize recent trends, focusing on research that moves away from traditional linkage studies. Some promising strategies include psychopharmacogenetics and consideration of endophenotypes such as neurophysiological and behavioral markers in addition to the clinical diagnosis. Utilization of rapid and automated methods for scoring genetic variants in large-scale …

Rapid Communication: Porcine Vitamin D-25-Hydroxylase Maps To Chromosome 5, Daniel C. Ciobanu, R. K. Raven, M. F. Rothschild, N. H. Bell

Department of Animal Science: Faculty Publications

Locus Name. Vitamin D-25-hydroxylase.

Species. Sus scrofa.

Locus Symbol. CYP25.

Source of Primers. PCR primers were designed from the published sequence for porcine vitamin D-25-hydroxylase (CYP25) cDNA (GenBank accession no. Y16417). The position of the forward and reverse primers corresponds to 506 to 530 bp and 830 to 854 bp, respectively, of the cDNA. The primer pair amplified a 348-bp fragment of the cDNA and an 862-bp fragment from the pig genomic DNA. The 862-bp fragment (Gen- Bank accession no. AF284378) was subcloned into a pNoTA vector and identity was confirmed by sequencing. Genomic sequences from 1 to 74, …

Comparison Of Plasma Fsh Concentration In Boars And Gilts From Lines Selected For Ovulation Rate And Embryonal Survival, And Litter Size And Estimation Of (Co)Variance Components For Fsh And Ovulation Rate, J. D. Cassady, Rodger K. Dennison, J. J. Ford

Department of Animal Science: Faculty Publications

The objective of this research was to determine whether plasma concentration of FSH was genetically correlated with ovulation rate and thus was a useful trait for indirect selection. Blood samples were collected from 619 animals from five lines of pigs. Line I was selected for increased index of ovulation rate and embryonal survival, and Line C was its randomly selected control. Pigs sampled from Lines I and C were from generations 12 and 13. Pigs from three additional lines that were derived from eighth-generation pigs of Lines I and C also were used. These lines were Line C2, a randomly …