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Full-Text Articles in Genetics and Genomics
Interactions Of Igf-Ii And Cathepsin D In Mcf-7 Breast Cancer Cells, Jesika S. Faridi
Interactions Of Igf-Ii And Cathepsin D In Mcf-7 Breast Cancer Cells, Jesika S. Faridi
Loma Linda University Electronic Theses, Dissertations & Projects
A primary role of the IGF-II/M6P receptor is to target lysosomal enzymes from the golgi to the lysosomes. This receptor has distinct binding sites for IGF-II and M6P, however, reciprocal interactions between these ligands have been observed (Kiess et al. 1989, 1990). Since IGF-II modulates the routing of cathepsin D in MCF-7 cells by blocking the intracellular binding of cathepsin D to the IGF-II/M6P receptor (De León et al. 1996), we hypothesized that expressing a mutant form of IGF-II that does not bind the IGF-II/M6P receptor will not interfere with lysosomal enzyme trafficking.
In our present study, we report the …
Expression And Requirement Of Epithelial Fatty Acid- Binding Protein In Neuronal Axon Growth, Gregory William Allen
Expression And Requirement Of Epithelial Fatty Acid- Binding Protein In Neuronal Axon Growth, Gregory William Allen
Loma Linda University Electronic Theses, Dissertations & Projects
Epithelial fatty acid-binding protein (E-FABP) is expressed in the dorsal root ganglia following sciatic nerve injury and in migrating and differentiating neurons during neuronal development. It was hypothesized that E-FABP expression is required for the robust outgrowth of axons from developing and regenerating neurons. To test this hypothesis, E-FABP expression in both PC12 cells and primary retinal neurons was examined. In PC12 cells, NGF induces E-FABP mRNA and protein during the period of neurite outgrowth, and E-FABP localizes to the perinuclear cytoplasm, nucleus, and growth cone. Furthermore, E-FABP-deficient cell lines exposed to NGF were less differentiated and had shorter neurites …
Molecular Cloning Of The Cdna For Porcine Parotid Hormone, Qian Zhang
Molecular Cloning Of The Cdna For Porcine Parotid Hormone, Qian Zhang
Loma Linda University Electronic Theses, Dissertations & Projects
It has been found that parotid glands secrete a factor into blood that stimulates dentinal fluid movement, which in turn, prevents the development of dental caries. This factor, designated as parotid hormone, has been isolated from porcine parotid glands and its partial amino acid sequence has been determined. This study was developed to isolate and identify the cDNA sequence for porcine parotid hormone.
A porcine parotid cDNA library was constructed and hybridized with oligo probes designed based on the partial amino acid sequences of the isolated porcine parotid hormone. Over 100 positive clones were isolated and the complete nucleotide sequences …