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Genetics and Genomics Commons

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Electronic Thesis and Dissertation Repository

2023

Cytochrome P450

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Full-Text Articles in Genetics and Genomics

Characterization Of Etoxazole Resistance In Tetranychus Urticae Populations Collected From Commercial Greenhouses & Fields In Ontario, Jeremy Spenler Jul 2023

Characterization Of Etoxazole Resistance In Tetranychus Urticae Populations Collected From Commercial Greenhouses & Fields In Ontario, Jeremy Spenler

Electronic Thesis and Dissertation Repository

In this investigation, etoxazole resistance was monitored in two spotted spider mite (TSSM) populations collected from commercial greenhouses in Ontario. The frequency of an etoxazole resistant, recessive target-site mutation, I1017F, in chitin synthase I (CHS1) was tracked within populations over the course of the study. Cytochrome P450 (P450) activity emerged as a possible alternative mechanism to resist etoxazole exposure in one population.

Using genetic crossing between highly resistant mite populations and a susceptible lab population, filial (F1) hybrid populations were created. The recessive I1017F target-site mutation was abolished in F1 hybrids highly susceptible to etoxazole, while elevated P450 …