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Full-Text Articles in Genetics and Genomics
Prevalence Of Mutations In The Pfdhfr, Pfdhps, And Pfmdr1 Genes Of Malarial Parasites Isolated From Symptomatic Patients In Dogondoutchi, Niger, Ibrahima Issa, Mahaman Moustapha Lamine, Veronique Hubert, Amadou Ilagouma, Eric Adehossi, Aboubacar Mahamadou, Neil F. Lobo, Demba Sarr, Lisa M. Shollenberger, Houze Sandrine, Ronan Jambou, Ibrahim Maman Laminou
Prevalence Of Mutations In The Pfdhfr, Pfdhps, And Pfmdr1 Genes Of Malarial Parasites Isolated From Symptomatic Patients In Dogondoutchi, Niger, Ibrahima Issa, Mahaman Moustapha Lamine, Veronique Hubert, Amadou Ilagouma, Eric Adehossi, Aboubacar Mahamadou, Neil F. Lobo, Demba Sarr, Lisa M. Shollenberger, Houze Sandrine, Ronan Jambou, Ibrahim Maman Laminou
Biological Sciences Faculty Publications
The effectiveness of artemisinin-based combination therapies (ACTs) depends not only on that of artemisinin but also on that of partner molecules. This study aims to evaluate the prevalence of mutations in the Pfdhfr, Pfdhps, and Pfmdr1 genes from isolates collected during a clinical study. Plasmodium genomic DNA samples extracted from symptomatic malaria patients from Dogondoutchi, Niger, were sequenced by the Sanger method to determine mutations in the Pfdhfr (codons 51, 59, 108, and 164), Pfdhps (codons 436, 437, 540, 581, and 613), and Pfmdr1 (codons 86, 184, 1034, and 1246) genes. One hundred fifty-five (155) pre-treatment samples were …
Gain-Of-Function Experiments With Bacteriophage Lambda Uncover Residues Under Diversifying Selection In Nature, Rohan Maddamsetti, Daniel T. Johnson, Stephanie J. Spielman, Katherine L. Petrie, Debora S. Marks, Justin R. Meyer
Gain-Of-Function Experiments With Bacteriophage Lambda Uncover Residues Under Diversifying Selection In Nature, Rohan Maddamsetti, Daniel T. Johnson, Stephanie J. Spielman, Katherine L. Petrie, Debora S. Marks, Justin R. Meyer
Biological Sciences Faculty Publications
Viral gain-of-function mutations frequently evolve during laboratory experiments. Whether the specific mutations that evolve in the lab also evolve in nature and whether they have the same impact on evolution in the real world is unknown. We studied a model virus, bacteriophage λ, that repeatedly evolves to exploit a new host receptor under typical laboratory conditions. Here, we demonstrate that two residues of λ’s J protein are required for the new function. In natural λ variants, these amino acid sites are highly diverse and evolve at high rates. Insertions and deletions at these locations are associated with phylogenetic patterns indicative …