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Articles 1 - 6 of 6
Full-Text Articles in Genetics and Genomics
A Mini-Tn5-Derived Transposon With Reportable And Selectable Markers Enables Rapid Generation And Screening Of Insertional Mutants In Gram-Negative Bacteria, Eric S. Nazareno, Bimala Acharya, C. Korsi Dumenyo
A Mini-Tn5-Derived Transposon With Reportable And Selectable Markers Enables Rapid Generation And Screening Of Insertional Mutants In Gram-Negative Bacteria, Eric S. Nazareno, Bimala Acharya, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
We re-engineered a classic tool for mutagenesis and gene expression studies in Gram-negative bacteria. Our modified Tn5-based transposon contains multiple features that allow rapid selection for mutants, direct quantification of gene expression and straightforward cloning of the inactivated gene. The promoter-less gfp-km cassette provides selection and reporter assay depending on the activity of the promoter upstream of the transposon insertion site. The cat gene facilitates positive antibiotic selection for mutants, while the narrow R6Kγ replication origin forces transposition in recipient strains lacking the pir gene and enables cloning of the transposon flanked with the disrupted gene from the chromosome. The …
Genotypic Variation Of Flavonols And Antioxidant Capacity In Broccoli, Yongbo Duan, Franklin Eduardo Melo Santiago, Andre Rodrigues Dos Reis, Marislaine A. De Figueiredo, Suping Zhou, Theodore W. Thannhauser, Li Li
Genotypic Variation Of Flavonols And Antioxidant Capacity In Broccoli, Yongbo Duan, Franklin Eduardo Melo Santiago, Andre Rodrigues Dos Reis, Marislaine A. De Figueiredo, Suping Zhou, Theodore W. Thannhauser, Li Li
Agricultural and Environmental Sciences Faculty Research
Flavonols are gaining increasing interests due to their diverse health benefits for humans. Broccoli is a main flavonol source in our diet, but the genetic variation of flavonols and their correlation with antioxidant capacity remain to be understood. Here, we examined variations of the two major flavonols kaempferol and quercetin in florets and leaves of 15 diverse broccoli accessions by ultra-performance liquid chromatography. Broccoli accumulated more kaempferol than quercetin in most of the accessions tested, with the ratios varying from 4.4 to 27.9 in leaves and 0.4 to 4.4 in florets. Total flavonoids showed 2.5-fold and 3.3-fold differences in leaves …
Genomic Modeling As An Approach To Identify Surrogates For Use In Experimental Validation Of Sars-Cov-2 And Hunov Inactivation By Uv-C Treatment, Brahmaiah Pendyala, Ankit Patras, Bharat Pokharel, Doris D’Souza
Genomic Modeling As An Approach To Identify Surrogates For Use In Experimental Validation Of Sars-Cov-2 And Hunov Inactivation By Uv-C Treatment, Brahmaiah Pendyala, Ankit Patras, Bharat Pokharel, Doris D’Souza
Agricultural and Environmental Sciences Faculty Research
Severe Acute Respiratory Syndrome coronavirus-2 (SARS-CoV-2) is responsible for the COVID-19 pandemic that continues to pose significant public health concerns. While research to deliver vaccines and antivirals are being pursued, various effective technologies to control its environmental spread are also being targeted. Ultraviolet light (UV-C) technologies are effective against a broad spectrum of microorganisms when used even on large surface areas. In this study, we developed a pyrimidine dinucleotide frequency based genomic model to predict the sensitivity of select enveloped and non-enveloped viruses to UV-C treatments in order to identify potential SARS-CoV-2 and human norovirus surrogates. The results revealed that …
Integrate Structural Analysis, Isoform Diversity, And Interferon-Inductive Propensity Of Ace2 To Predict Sars-Cov2 Susceptibility In Vertebrates, Eric R. Sang, Yun Tian, Yuanying Gong, Laura C. Miller, Yongming Sang
Integrate Structural Analysis, Isoform Diversity, And Interferon-Inductive Propensity Of Ace2 To Predict Sars-Cov2 Susceptibility In Vertebrates, Eric R. Sang, Yun Tian, Yuanying Gong, Laura C. Miller, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
The current new coronavirus disease (COVID-19) has caused globally over 0.4/6 million confirmed deaths/infected cases across more than 200 countries. As the etiological coronavirus (a.k.a. SARS-CoV2) may putatively have a bat origin, our understanding about its intermediate reservoir between bats and humans, especially its tropism in wild and domestic animals are mostly unknown. This constitutes major concerns in public health for the current pandemics and potential zoonosis. Previous reports using structural analysis of the viral spike protein (S) binding its cell receptor of angiotensin-converting enzyme 2 (ACE2), indicate a broad potential of SARS-CoV2 susceptibility in wild and particularly domestic animals. …
Proteome Profile Changes During Poly-Hydroxybutyrate Intracellular Mobilization In Gram Positive Bacillus Cereus Tsu1, Hui Li, Joshua O'Hair, Santosh Thapa, Sarabjit Bhatti, Suping Zhou, Yong Yang, Tara Fish, Theodore W. Thannhauser
Proteome Profile Changes During Poly-Hydroxybutyrate Intracellular Mobilization In Gram Positive Bacillus Cereus Tsu1, Hui Li, Joshua O'Hair, Santosh Thapa, Sarabjit Bhatti, Suping Zhou, Yong Yang, Tara Fish, Theodore W. Thannhauser
Agricultural and Environmental Sciences Faculty Research
Bacillus cereus is a bacterial species which grows efficiently on a wide range of carbon sources and accumulates biopolymer poly-hydroxybutyrate (PHB) up to 80% cell dry weight. PHB is an aliphatic polymer produced and stored intracellularly as a reservoir of carbon and energy, its mobilization is a key biological process for sporulation in Bacillus spp. Previously, B. cereus tsu1 was isolated and cultured on rapeseed cake substrate (RCS), with maximum of PHB accumulation reached within 12 h, and depleted after 48 h. Fore-spore and spore structure were observed after 24 h culture.
Expression Level Of Sh2 And Bt2 Genes In Some Advanced Corn Lines Under Tropical Environment, Fatemeh Abna, Farhat A. Avin, Noorma Wati Haron
Expression Level Of Sh2 And Bt2 Genes In Some Advanced Corn Lines Under Tropical Environment, Fatemeh Abna, Farhat A. Avin, Noorma Wati Haron
Agricultural and Environmental Sciences Faculty Research
The rate-limiting step in starch synthesis entails the synthesis of ADP-Glc from Glc-1-P and ATP by AGPase. AGPase is a heterotetramer composed of two large subunits encoded by Sh2 and two small subunits encoded by Bt2. We examined the transcript level of each gene that encodes AGPase through quantitative real-time PCR (qRT_PCR) within endosperm and leaf tissues from 6 newer composite corn lines. The Sh2 and Bt2 genes presented upregulated expression levels in leaf and endosperm tissue. The highest expression level of these two genes in leaf tissues was observed in UM 1 with 6.96 fold and in UM 11 …