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Full-Text Articles in Genetics and Genomics
Localization Of Sumo-Modified Proteins Using Fluorescent Sumo-Trapping Proteins, Rui Yin, Catherine Harvey, Diane C. Shakes, Oliver Kerscher
Localization Of Sumo-Modified Proteins Using Fluorescent Sumo-Trapping Proteins, Rui Yin, Catherine Harvey, Diane C. Shakes, Oliver Kerscher
Arts & Sciences Articles
Here we are presenting a novel method to study the sumoylation of proteins and their sub-cellular localization in mammalian cells and nematode oocytes. This method utilizes a recombinant modified SUMO-trapping protein fragment, kmUTAG, derived from the Ulp1 SUMO protease of the stress-tolerant budding yeast Kluyveromyces marxianus. We have adapted the properties of the kmUTAG for the purpose of studying sumoylation in a variety of model systems without the use of antibodies. For the study of SUMO, KmUTAG has several advantages when compared to antibody-based approaches. This stress-tolerant SUMO-trapping reagent is produced recombinantly, it recognizes native SUMO isoforms from many …
Fecundity Of Triploid Eastern Oyster (Crassostrea Virginica) As A Function Of Tetraploid Lineage, Kate Ritter
Fecundity Of Triploid Eastern Oyster (Crassostrea Virginica) As A Function Of Tetraploid Lineage, Kate Ritter
Dissertations, Theses, and Masters Projects
Rapid growth rate and partial sterility have made triploid eastern oysters (Crassostrea virginica) the most popular crop variety for the Virginia oyster aquaculture industry, typically comprising greater than 90% of larvae and juvenile sales. Triploid advantages, however, sometimes come with the disadvantage of higher mortality in late spring and early summer, dubbed “triploid mortality.” In recent years, farms up and down the East Coast, especially Maryland and south into the Gulf of Mexico have experienced triploid mortality. Some of the reports include observations of elevated triploid fecundity. Triploid oysters are created by crossing tetraploids to diploids, and much of the …