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Full-Text Articles in Genetics and Genomics
Embryogenic Cell Suspensions For High-Capacity Genetic Transformation And Regeneration Of Switchgrass (Panicum Virgatum L.), Christine A. Ondzighi-Assoume, Jonathan D. Willis, Wilson Kihugu Ouma, Sara M. Allen, Zachary King, Wayne A. Parrott, Wusheng Liu, Jason N. Burris, Scott C. Lenaghan, C. Neal Stewart
Embryogenic Cell Suspensions For High-Capacity Genetic Transformation And Regeneration Of Switchgrass (Panicum Virgatum L.), Christine A. Ondzighi-Assoume, Jonathan D. Willis, Wilson Kihugu Ouma, Sara M. Allen, Zachary King, Wayne A. Parrott, Wusheng Liu, Jason N. Burris, Scott C. Lenaghan, C. Neal Stewart
Agricultural and Environmental Sciences Faculty Research
Background
Switchgrass (Panicum virgatum L.), a North American prairie grassland species, is a potential lignocellulosic biofuel feedstock owing to its wide adaptability and biomass production. Production and genetic manipulation of switchgrass should be useful to improve its biomass composition and production for bioenergy applications. The goal of this project was to develop a high-throughput stable switchgrass transformation method using Agrobacterium tumefaciens with subsequent plant regeneration.
Results
Regenerable embryogenic cell suspension cultures were established from friable type II callus-derived inflorescences using two genotypes selected from the synthetic switchgrass variety ‘Performer’ tissue culture lines 32 and 605. The cell suspension cultures …