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- Acetyl CoA deficiency (1)
- Chicken flavoproteome (1)
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- Feather keratin (1)
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- Riboflavin deficiency (1)
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Articles 1 - 2 of 2
Full-Text Articles in Genetics and Genomics
Transcriptional Profiling Of Liver During The Critical Embryo-To-Hatchling Transition Period In The Chicken (Gallus Gallus), Larry A. Cogburn, Nares Trakooljul, Chuming Chen, Hongzhan Huang, Cathy H. Wu, Wilfrid Carré, Xiaofei Wang, Harold B. White Iii
Transcriptional Profiling Of Liver During The Critical Embryo-To-Hatchling Transition Period In The Chicken (Gallus Gallus), Larry A. Cogburn, Nares Trakooljul, Chuming Chen, Hongzhan Huang, Cathy H. Wu, Wilfrid Carré, Xiaofei Wang, Harold B. White Iii
Biology Faculty Research
Background
Although hatching is perhaps the most abrupt and profound metabolic challenge that a chicken must undergo; there have been no attempts to functionally map the metabolic pathways induced in liver during the embryo-to-hatchling transition. Furthermore, we know very little about the metabolic and regulatory factors that regulate lipid metabolism in late embryos or newly-hatched chicks. In the present study, we examined hepatic transcriptomes of 12 embryos and 12 hatchling chicks during the peri-hatch period—or the metabolic switch from chorioallantoic to pulmonary respiration.
Results
Initial hierarchical clustering revealed two distinct, albeit opposing, patterns of hepatic gene expression. Cluster A genes …
Transcriptional Profiling Of Liver In Riboflavin-Deficient Chicken Embryos Explains Impaired Lipid Utilization, Energy Depletion, Massive Hemorrhaging, And Delayed Feathering, Larry A. Cogburn, Danielle N. Smarsh, Xiaofei Wang, Nares Trakooljul, Wilfrid Carré, Harold B. White Iii
Transcriptional Profiling Of Liver In Riboflavin-Deficient Chicken Embryos Explains Impaired Lipid Utilization, Energy Depletion, Massive Hemorrhaging, And Delayed Feathering, Larry A. Cogburn, Danielle N. Smarsh, Xiaofei Wang, Nares Trakooljul, Wilfrid Carré, Harold B. White Iii
Biology Faculty Research
Background
A strain of Leghorn chickens (rd/rd), unable to produce a functional riboflavin-binding protein, lays riboflavin-deficient eggs, in which all embryos suddenly die at mid-incubation (days 13-15). This malady, caused by riboflavin deficiency, leads to excessive lipid accumulation in liver, impaired β-oxidation of lipid, and severe hypoglycemia prior to death. We have used high-density chicken microarrays for time-course transcriptional scans of liver in chicken embryos between days 9-15 during this riboflavin-deficiency-induced metabolic catastrophe. For comparison, half of rd/rd embryos (n = 16) were rescued from this calamity by injection of riboflavin just prior to incubation of fertile …