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Articles 1 - 8 of 8
Full-Text Articles in Genetics and Genomics
Sperm-Mediated Effects Of Predation Risk On Reproduction In Male Threespined Sticklebacks, Shayne Kempfer, Erika Carlson, Jennifer Hellmann, Jason Keagy, Alison Bell
Sperm-Mediated Effects Of Predation Risk On Reproduction In Male Threespined Sticklebacks, Shayne Kempfer, Erika Carlson, Jennifer Hellmann, Jason Keagy, Alison Bell
PRECS student projects
Threespined sticklebacks, Gasterosteus aculeatus, exhibit male-only parental care, but will decrease paternal care if exposed to predators while given care or if their mate has had prior experiences with predators. The offspring will experience lasting effects based on the experience of their parents (behavior-mediated transgenerational plasticity). While other studies have concluded that male exposure to predators prior to fertilization (sperm-mediated transgenerational plasticity) impacts offspring, whether sperm-mediated effects exist in sticklebacks, and for how many generations the effects persist is unknown. Courtship trials with both predator exposed and predator unexposed females with six male treatment groups shows significant impact of maternal …
Production Of Bioactive Isothiocyanates By Bacterial Myrosinase, Juan Isaiz, Xiaoji Liu, Michael J. Miller
Production Of Bioactive Isothiocyanates By Bacterial Myrosinase, Juan Isaiz, Xiaoji Liu, Michael J. Miller
PRECS student projects
Isothiocyanates (ITCs) are bioactive products found in several plants belonging to the Brassicaceae family, and are produced as defense upon disruption of the tissue by herbivores or pathogens, but in humans they have shown anti-cancer and anti-inflammatory properties.
Although we know gut microbes can hydrolyze GSL into ITC, the mechanism is not known. With improved detection methods, the aim of this experiment is to explore whether the gut microbial myrosinase β-glucosidase found within the genome of E. coli Nissle 1917 can hydrolyze glucosinolates (GSLs) into detectable ITCs.
Finding Dex-1 Phenotype Suppressing Components, Matthew Barnum, Kristen Flatt, Nathan E. Schroeder
Finding Dex-1 Phenotype Suppressing Components, Matthew Barnum, Kristen Flatt, Nathan E. Schroeder
PRECS 2018
Caenorhabditis elegans is a species of microscopic round worm that has been used as a genetic model for over forty years. When in an adverse environment, C. elegans larvae cease reproductive development and enter the stress-resistant dauer stage.
dex-1 mutants of C. elegans are deficient in this protein, resulting in shortened dendrites and a sensitivity to sodium dodecyl sulfate (SDS). SDS will kill any non-dauer C. elegans, but wild type dauers will survive well past the standard concentration of 1% SDS. Thus, treatment with SDS is commonly how labs isolate dauers. By contrast, dex-1 dauers (Fig.3) will …
Identifying Kif Subtype That Mediates Axonal Targeting Of Kv7 Channels, Allison Houghton, Jennifer Walters, Mary Hong, Dhruv Joshi, Hee Jung Chung
Identifying Kif Subtype That Mediates Axonal Targeting Of Kv7 Channels, Allison Houghton, Jennifer Walters, Mary Hong, Dhruv Joshi, Hee Jung Chung
PRECS 2018
Early-onset Benign Familial Neonatal Epilepsy (BFNE) and Epileptic Encephalopathy (EE), are associated with mutations in neuronal KCNQ/Kv7 channel subunits Kv7.2 and Kv7.3. Kv7 channels are voltage-dependent potassium channels. Enriched at the axonal plasma membrane, they pump potassium ions out of the neurons and inhibit repetitive or burst firing of action potentials. A single neuronal Kv7 channel is a heterotetramer composed of two Kv7.2 and two Kv7.3 subunits. BFNE and EE mutations in Kv7.2 and Kv7.3 lead to decreased surface expression along the axon, which means less potassium ions are moved across the axonal membrane where action potentials are generated and …
The Effect Of Imidacloprid On Honey Bee Queen Fecundity, Jamilyn Martin, Julia D. Fine, Amy Cash-Ahmed, Gene E. Robinson
The Effect Of Imidacloprid On Honey Bee Queen Fecundity, Jamilyn Martin, Julia D. Fine, Amy Cash-Ahmed, Gene E. Robinson
PRECS 2018
Imidacloprid is a neonicotinoid insecticide commonly used in agricultural settings to control insect pests by acting as an agonist of acetylcholine receptors and inducing paralysis and mortality. In small doses, imidacloprid can cause loss of memory and foraging ability along with impaired learning and a lowered immune response in western honey bees (Apis mellifera). Effects of neonicotinoid insecticides on colony reproduction have been documented including decreased colony expansion, queen failure and replacement, and decreased queen egg laying.
For this study, we examined the effects of imidacloprid on the fecundity of queen bees when their worker attendants were exposed to low …
Methamphetamine Effects On Adolescent Brain Development In Both Sexes, Jessica Alvarez, Sarah Westbrook, Joshua Gulley
Methamphetamine Effects On Adolescent Brain Development In Both Sexes, Jessica Alvarez, Sarah Westbrook, Joshua Gulley
PRECS 2018
Vulnerability to drug addiction in adolescents (ADOLs) may lead to faster rates of addiction and higher rates of relapse. The brain is developing in adolescence, including the prefrontal cortex (PFC) and the nucleus accumbens (NAc), which are regions involved in self-control and reward systems, respectively. Further, females, both human and rat models, often exhibit faster rates of addiction than males. Research from this lab has found previously that adult rats of both sexes exposed to amphetamine during ADOL had reduced expression of dopamine D1 receptors (D1R) in the medial PFC but no change in the NAc. The …
How Does Climate Change Affect Endophytic Fungi?, Amber Toussaint, Natalie Christian, Katy Heath
How Does Climate Change Affect Endophytic Fungi?, Amber Toussaint, Natalie Christian, Katy Heath
PRECS student projects
Plant-associated microorganisms can have major effects on plant health, but climate change can affect plant microbiomes. While it is unknown how climate change will affect aboveground plant-associated microorganisms, such as foliar endophytic fungi, we hypothesize that increased CO2 concentrations will increase species richness and diversity in endophytic fungal communities, because increased plant photosynthesis under elevated CO2 will increase the resources available to the fungi.
Phosphorus Vs. Mycorrhiza: Mitigation Of Lead Uptake In Tomatoes, Helen Senerchia, Liz Miernicki, Andrew Margenot
Phosphorus Vs. Mycorrhiza: Mitigation Of Lead Uptake In Tomatoes, Helen Senerchia, Liz Miernicki, Andrew Margenot
PRECS 2018
This study seeks to understand how the mutualist relationship between arbuscular mycorrhizal fungi (AM) and Solanum lycopersicum (tomato) affects the uptake and distribution of iron (Pb) found in soil by tomatoes and how the effects of phosphorous (P) application may pose a trade-off between AM and P mitigation of bioavailability of Pb.