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Full-Text Articles in Genetics and Genomics
Investigation Of Notch Signaling In Cone Fate Specification In Vertebrate Retina, Xueqing Chen
Investigation Of Notch Signaling In Cone Fate Specification In Vertebrate Retina, Xueqing Chen
Dissertations, Theses, and Capstone Projects
In the vertebrate retina, cone photoreceptors are crucial for high acuity color vision. Several retinal diseases lead to loss of cones and there is a need to identify the normal developmental genesis of these cells to inform the development of stem cell-based therapies. Cone genesis has previously been shown to be repressed by Notch signaling, however, the mechanism by which Notch signaling controls cone fate determination is still unclear. It has been identified that cone photoreceptors are formed from multipotent retinal progenitor cells (RPCs) that first generate genetically-defined, restricted RPCs with limited mitotic and fate potential to preferentially form cones …
Gene Regulation And Cell Fate Choice In The Developing Vertebrate Retina, Sruti Patoori
Gene Regulation And Cell Fate Choice In The Developing Vertebrate Retina, Sruti Patoori
Dissertations, Theses, and Capstone Projects
The diverse neuronal cell types in the vertebrate retina all originate from multipotent retinal progenitor cells (RPCs). These undergo a series of molecular changes driven by developmental gene regulatory networks (GRNs) as they divide to generate RPCs which are more restricted in their potential fates. It is crucial to understand these GRNs and changes to gene expression in order to understand how cell identity is established during retinal development. In particular, the GRN that promotes the development of cone photoreceptors and horizontal cells is not well-defined. This work focuses on two approaches to further elucidate the components of this regulatory …
Quantitative Analysis Of The Thrbcrm1-Centered Gene Regulatory Network, Benjamin Souferi, Mark M. Emerson
Quantitative Analysis Of The Thrbcrm1-Centered Gene Regulatory Network, Benjamin Souferi, Mark M. Emerson
Publications and Research
Enhancer activity is determined by both the activity and occupancy of transcription factors as well as the specific sequences they bind. Experimental investigation of this dynamic requires the ability to manipulate components of the system, ideally in as close to an in vivo context as possible. Here we use electroporation of plasmid reporters to define critical parameters of a specific cis-regulatory element, ThrbCRM1, during retinal development. ThrbCRM1 is associated with cone photoreceptor genesis and activated in a subset of developing retinal cells that co-express the Otx2 and Onecut1 (OC1) transcription factors. Variation of reporter plasmid concentration was used to generate …