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Genetics and Genomics Commons

Open Access. Powered by Scholars. Published by Universities.®

City University of New York (CUNY)

2015

De novo assembly

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Full-Text Articles in Genetics and Genomics

Transcriptome Sequencing And Annotation Of The Polychaete Hermodice Carunculata (Annelida, Amphinomidae), Shaadi Mehr, Aida Verdes, Rob Desalle, John S. Sparks, Vincent A. Pieribone, David F. Gruber Jun 2015

Transcriptome Sequencing And Annotation Of The Polychaete Hermodice Carunculata (Annelida, Amphinomidae), Shaadi Mehr, Aida Verdes, Rob Desalle, John S. Sparks, Vincent A. Pieribone, David F. Gruber

Publications and Research

Background: The amphinomid polychaete Hermodice carunculata is a cosmopolitan and ecologically important omnivore in coral reef ecosystems, preying on a diverse suite of reef organisms and potentially acting as a vector for coral disease. While amphinomids are a key group for determining the root of the Annelida, their phylogenetic position has been difficult to resolve, and their publically available genomic data was scarce.

Results: We performed deep transcriptome sequencing (Illumina HiSeq) and profiling on Hermodice carunculata collected in the Western Atlantic Ocean. We focused this study on 58,454 predicted Open Reading Frames (ORFs) of genes longer than 200 amino acids …