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Full-Text Articles in Genetics and Genomics
A Mini-Tn5-Derived Transposon With Reportable And Selectable Markers Enables Rapid Generation And Screening Of Insertional Mutants In Gram-Negative Bacteria, Eric S. Nazareno, Bimala Acharya, C. Korsi Dumenyo
A Mini-Tn5-Derived Transposon With Reportable And Selectable Markers Enables Rapid Generation And Screening Of Insertional Mutants In Gram-Negative Bacteria, Eric S. Nazareno, Bimala Acharya, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
We re-engineered a classic tool for mutagenesis and gene expression studies in Gram-negative bacteria. Our modified Tn5-based transposon contains multiple features that allow rapid selection for mutants, direct quantification of gene expression and straightforward cloning of the inactivated gene. The promoter-less gfp-km cassette provides selection and reporter assay depending on the activity of the promoter upstream of the transposon insertion site. The cat gene facilitates positive antibiotic selection for mutants, while the narrow R6Kγ replication origin forces transposition in recipient strains lacking the pir gene and enables cloning of the transposon flanked with the disrupted gene from the chromosome. The …
Cross-Species Genome-Wide Analysis Reveals Molecular And Functional Diversity Of The Unconventional Interferon-Ω Subtype, Lauren E. Shields, Jordan Jennings, Qinfang Liu, Jinhwa Lee, Wenjun Ma, Frank Blecha, Laura C. Miller, Yongming Sang
Cross-Species Genome-Wide Analysis Reveals Molecular And Functional Diversity Of The Unconventional Interferon-Ω Subtype, Lauren E. Shields, Jordan Jennings, Qinfang Liu, Jinhwa Lee, Wenjun Ma, Frank Blecha, Laura C. Miller, Yongming Sang
Agricultural and Environmental Sciences Faculty Research
Innate immune interferons (IFNs), particularly type I IFNs, are primary mediators regulating animal antiviral, antitumor, and cell-proliferative activity. These antiviral cytokines have evolved remarkable molecular and functional diversity to confront ever-evolving viral threats and physiological regulation. We have annotated IFN gene families across 110 animal genomes, and showed that IFN genes, after originating in jawed fishes, had several significant evolutionary surges in vertebrate species of amphibians, bats and ungulates, particularly pigs and cattle. For example, pigs have the largest but still expanding type I IFN family consisting of nearly 60 IFN-coding genes that encode seven IFN subtypes including multigene subtypes …
Draft Genome Sequence Of Bacillus Altitudinis Ynp4-Tsu, Isolated From Yellowstone National Park, Joshua A. O'Hair, Hui Li, Santosh Thapa, Matthew Scholz, Suping Zhou
Draft Genome Sequence Of Bacillus Altitudinis Ynp4-Tsu, Isolated From Yellowstone National Park, Joshua A. O'Hair, Hui Li, Santosh Thapa, Matthew Scholz, Suping Zhou
Agricultural and Environmental Sciences Faculty Research
Undisturbed hot springs inside Yellowstone National Park remain a dynamic biome for novel cellulolytic thermophiles. We report here the draft genome sequence of one of these isolates, Bacillus altitudinis YNP4-TSU.
Draft Genome Sequences Of Three Cellulolytic Bacillus Licheniformis Strains Isolated From Imperial Geyser, Amphitheater Springs, And Whiterock Springs Inside Yellowstone National Park, Joshua A. O'Hair, Hui Li, Santosh Thapa, Matthew Scholz, Suping Zhou
Draft Genome Sequences Of Three Cellulolytic Bacillus Licheniformis Strains Isolated From Imperial Geyser, Amphitheater Springs, And Whiterock Springs Inside Yellowstone National Park, Joshua A. O'Hair, Hui Li, Santosh Thapa, Matthew Scholz, Suping Zhou
Agricultural and Environmental Sciences Faculty Research
Novel cellulolytic microorganisms are becoming more important for rapidly growing biofuel industries. This paper reports the draft genome sequences of Bacillus licheniformis strains YNP2-TSU, YNP3-TSU, and YNP5-TSU. These cellulolytic isolates were collected from several hydrothermal features inside Yellowstone National Park.
Draft Genome Sequence Of New Bacillus Cereus Strain Tsu1, Hui Li, Suping Zhou, Terrance Johnson, Koen Vercruysse, Alexander J. Ropelewski, Theodore W. Thannhauser
Draft Genome Sequence Of New Bacillus Cereus Strain Tsu1, Hui Li, Suping Zhou, Terrance Johnson, Koen Vercruysse, Alexander J. Ropelewski, Theodore W. Thannhauser
Agricultural and Environmental Sciences Faculty Research
This paper reports the draft genome sequence of new Bacillus cereus strain tsu1, isolated on an agar-cellulose plate. The draft genome sequence is 5.81 Mb, revealing 5,673 coding sequences. It contains genes for cellulose-degradation and biosynthesis pathways of polyhydroxybutyrate (PHB) and 8 rRNA genes (5S, 16S, and 23S).