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Cytologic Characterization Of Human Constitutive Heterochromatin, Timothy Atchison Donlon
Cytologic Characterization Of Human Constitutive Heterochromatin, Timothy Atchison Donlon
Dissertations and Theses
A study was conducted to cytologically map certain subsets of constitutive heterochromatin onto specific portions of human chromosomes. This involved sequentially staining metaphase chromosomes from lymphocytes first with the Centromeric Dot, Giemsa-11, G-banding or Lateral Asymmetry staining techniques, which are cytochemical methods which stain particular chromosomes or chromosomal regions. Then those same metaphase chromosomes were stained using the C-banding technique, a method which is believed to denote constitutive heterochromatin. With the exception of the G-banding technique, areas depicted by the special staining techniques were found to reside only within the areas stained by the C-band technique and are thus believed …