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Full-Text Articles in Genetics and Genomics
Application Of Genetic Markers To Provide Species Identification And Define Stock Structure: Analyses Of Selected Marine Fishes Of The Mid -Atlantic Bight, Jan F. Cordes
Dissertations, Theses, and Masters Projects
Molecular markers and techniques were employed to develop a genetic key for the forensic identification of 16 species of Chesapeake Bay sportfishes and to investigate the stock structure of one of those species, the weakfish Cynoscion regalis. Regions within the ATP synthetase 6 (ATPase 6), Cytochrome b , cytochrome c oxidase I, NADH dehydrogenase 4 (ND4), and 12S/16S ribosomal RNA mitochondrial genes were amplified using the polymerase chain reaction (PCR) and digested with a bank of restriction endonucleases to find a genetic marker that exhibited complete interspecific differentiation and low intraspecific variation. Complete separation of all sixteen species was accomplished …