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Full-Text Articles in Genetics and Genomics
Validation Of A Short Tandem Repeat Multiplex Typing System For Genetic Individualization Of Domestic Cat Samples, Nikia Coomber, Victor A. David, Stephen J. O'Brien, Marilyn Menotti-Raymond
Validation Of A Short Tandem Repeat Multiplex Typing System For Genetic Individualization Of Domestic Cat Samples, Nikia Coomber, Victor A. David, Stephen J. O'Brien, Marilyn Menotti-Raymond
Biology Faculty Articles
Aim To conduct developmental validation studies on a polymerase chain reaction (PCR) based short tandem repeat (STR) multiplex typing system, developed for the purpose of genetic individualization and parentage testing in domestic cat samples.
Methods To evaluate reproducibility of the typing system, the multiplex was amplified using DNA extracted from hair, blood, and buccal samples obtained from the same individual (n = 13). Additional studies were performed to evaluate the system’s species’ specificity, using 26 North American mammalian species and two prokaryotes Sacchromyces and Escherichia coli, sensitivity, and ability to identify DNA mixtures. Patterns of Mendelian inheritance and mutation …