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Articles 1 - 19 of 19
Full-Text Articles in Genetics and Genomics
Identification Of Eurycea Using Cytochrome B, Karis A. Myers
Identification Of Eurycea Using Cytochrome B, Karis A. Myers
Senior Honors Theses
Genomic sequencing is a powerful tool that has many applications for research, one of which is in the field of taxonomy and the identification of species. This thesis discusses the mitochondrial gene cytochrome b and its utility in population genetics and identification of larval amphibians. The development of the Polymerase Chain Reaction and primers are an integral part of the modern DNA sequencing process. The Polymerase Chain Reaction is used to amplify a target DNA sequence, and the protocol for this procedure must be optimized for the specific sequence of target DNA. Primers must also be designed and modified for …
Attempted Cloning Of A Wnt Gene From Botrylloides Violaceus, Manasa Chandra, James Tumulak
Attempted Cloning Of A Wnt Gene From Botrylloides Violaceus, Manasa Chandra, James Tumulak
Biological Sciences
Botrylloides violaceus is a colonial ascidian with the ability to undergo sexual and asexual reproduction as well as regeneration. The canonical pathway starts with the extracellular protein Wnt and ends with β-catenin, a transcription factor, which also functions in cell adhesion. The Wnt signaling pathway is involved in embryogenesis and regeneration in a variety of other species. In our studies we attempt to isolate and sequence both a Wnt gene and from Botrylloides via degenerate primer design and PCR. Using bioinformatic methods we aligned sequences from other organisms, as the Botrylloides genome has not yet been sequenced. Using mouse, Ciona, …
Aspen In Scotland: Biodiversity And Management, John Parrott, Neil Mackenzie
Aspen In Scotland: Biodiversity And Management, John Parrott, Neil Mackenzie
Aspen Bibliography
No abstract provided.
It's Not Too Late For The Harpy Eagle (Harpia Harpyja): High Levels Of Genetic Diversity And Differentiation Can Fuel Conservation Programs, Heather R. Lerner, Jeff A. Johnson, Alec R. Lindsay Ph. D., Lloyd F. Kiff, David P. Mindell
It's Not Too Late For The Harpy Eagle (Harpia Harpyja): High Levels Of Genetic Diversity And Differentiation Can Fuel Conservation Programs, Heather R. Lerner, Jeff A. Johnson, Alec R. Lindsay Ph. D., Lloyd F. Kiff, David P. Mindell
Faculty Works
Background: The harpy eagle (Harpia harpyja) is the largest Neotropical bird of prey and is threatened by human persecution and habitat loss and fragmentation. Current conservation strategies include local education, captive rearing and reintroduction, and protection or creation of trans-national habitat blocks and corridors. Baseline genetic data prior to reintroduction of captive-bred stock is essential for guiding such efforts but has not been gathered previously.
Methodology/Findings: We assessed levels of genetic diversity, population structure and demographic history for harpy eagles using samples collected throughout a large portion of their geographic distribution in Central America (n = 32) and South America …
Assessment Of The Endangered Species Podarcis Carbonelli On A Microgeographic Scale: A Molecular, Morphological And Physiological Approach, Maria Clara Figueirinhas Do Amaral
Assessment Of The Endangered Species Podarcis Carbonelli On A Microgeographic Scale: A Molecular, Morphological And Physiological Approach, Maria Clara Figueirinhas Do Amaral
Masters Theses & Specialist Projects
The lizard Podarcis carbonelli is an endangered species endemic to the Iberian Peninsula. One location where this species occurs is at the Berlengas Natural Preserve, an Atlantic archipelago off the coast of Portugal. These island populations are geographically separated from nearby mainland populations. The fundamental question is, are these insular individuals distinct from the mainland populations? Four localities were chose for comparison: two island populations and two nearby coastal populations. We assessed this question using three distinct approaches: molecular, morphological and physiological approach. We sequenced the 12S RNA, the mtDNA Control Region and the 7th intron of the !-fibrinogen gene …
Road-Killed Bats, Highway Design, And The Commuting Ecology Of Bats, Amy L. Russell, Calvin M. Butchkoski, Leslie Saidak, Gary F. Mccracken
Road-Killed Bats, Highway Design, And The Commuting Ecology Of Bats, Amy L. Russell, Calvin M. Butchkoski, Leslie Saidak, Gary F. Mccracken
Amy L. Russell
A Phylogenetic Analysis Of Armored Scale Insects, Based Upon Nuclear, Mitochondrial, And Endosymbiont Gene Sequences, Jeremy C. Andersen
A Phylogenetic Analysis Of Armored Scale Insects, Based Upon Nuclear, Mitochondrial, And Endosymbiont Gene Sequences, Jeremy C. Andersen
Masters Theses 1911 - February 2014
Armored scale insects (Hemiptera: Diaspididae) are among the most invasive insects in the world. They have unusual genetic systems, including diverse types of paternal genome elimination (PGE) and parthenogenesis. Intimate relationships with their host plants and bacterial endosymbionts make them potentially important subjects for the study of co- evolution. Also, in some groups, the adult female never sheds the second instars cuticle, and remains within its confines, a habit referred to as the pupillarial habit. Here we expand upon recent phylogenetic work (Morse and Normark 2006) by analyzing a partitioned dataset including armored scale and endoysmbiont DNA from one hundred …
Characterization Of 24 Microsatellite Loci In Delta Smelt, Hypomesus Transpacificus, And Their Cross-Species Amplification In Two Other Smelt Species Of The Osmeridae Family, Kathleen M. Fisch, Jessica Lynn Petersen, Melinda R. Baerwald, John K. Pedroia, Bernie May
Characterization Of 24 Microsatellite Loci In Delta Smelt, Hypomesus Transpacificus, And Their Cross-Species Amplification In Two Other Smelt Species Of The Osmeridae Family, Kathleen M. Fisch, Jessica Lynn Petersen, Melinda R. Baerwald, John K. Pedroia, Bernie May
Department of Animal Science: Faculty Publications
We characterized 24 polymorphic tetranucleotide microsatellite loci for delta smelt (Hypomesus transpacificus) endemic to the San Francisco Bay Estuary, California, USA. Screening of samples (n = 30) yielded two to 26 alleles per locus with observed levels of heterozygosity ranging from 0.17 to 1.0. Only one locus deviated from Hardy–Weinberg equilibrium, suggesting these individuals originate from a single panmictic population. Linkage disequilibrium was found in two pairs of loci after excluding the locus out of Hardy–Weinberg equilibrium. Twenty-two primer pairs cross-amplified in wakasagi smelt (Hypomesus nipponensis), and 15 primer pairs cross-amplified in longfin smelt ( …
Primate Phylogenomics: Developing Numerous Nuclear Non-Coding, Non-Repetitive Markers For Ecological And Phylogenetic Applications And Analysis Of Evolutionary Rate Variation, Zuogang Peng, Navin Elango, Derek E. Wildman, Soojin V. Yi
Primate Phylogenomics: Developing Numerous Nuclear Non-Coding, Non-Repetitive Markers For Ecological And Phylogenetic Applications And Analysis Of Evolutionary Rate Variation, Zuogang Peng, Navin Elango, Derek E. Wildman, Soojin V. Yi
Wayne State University Associated BioMed Central Scholarship
Abstract
Background
Genetic analyses are often limited by the availability of appropriate molecular markers. Markers from neutrally evolving genomic regions may be particularly useful for inferring evolutionary histories because they escape the constraints of natural selection. For the majority of taxa however, obtaining such markers is challenging. Advances in genomics have the potential to alleviate the shortage of neutral markers. Here we present a method to develop numerous markers from putatively neutral regions of primate genomes.
Results
We began with the available whole genome sequences of human, chimpanzee and macaque. Using computational methods, we identified a total of 280 potential …
A New Method For Collecting Clean Stable Fly (Diptera: Muscidae) Pupae Of Known Age., Dennis R. Berkebile, Anthony P. Weinhold, David B. Taylor
A New Method For Collecting Clean Stable Fly (Diptera: Muscidae) Pupae Of Known Age., Dennis R. Berkebile, Anthony P. Weinhold, David B. Taylor
David B Taylor
Stable flies, Stomoxys calcitrans L., are important pests of confined and pasture cattle. They have been reared in the laboratory to study their biology and to test new methods of control. Research on rearing modifications has concentrated on developing larval diets from materials locally abundant. Under current protocols, pupae form in the medium. Aggregations of pupae were located and removed, often with a considerable amount of extraneous material. Various methods have been developed to separate the pupae from waste material. We describe a method by which wandering larvae are enticed to leave the medium prior to pupariation. The larvae were …
Thirty-Seven Additional Microsatellite Loci In The Pacific Lion-Paw Scallop (Nodipecten Subnodosus) And Cross-Amplification In Other Pectinids, Jessica Lynn Petersen, Ana M. Ibarra, Bernie May
Thirty-Seven Additional Microsatellite Loci In The Pacific Lion-Paw Scallop (Nodipecten Subnodosus) And Cross-Amplification In Other Pectinids, Jessica Lynn Petersen, Ana M. Ibarra, Bernie May
Department of Animal Science: Faculty Publications
We characterized 37 new microsatellite markers in the Pacific lion-paw scallop (Nodipecten subnodosus) and tested for cross-amplification in four other species. Genetic diversity was estimated using 24 individuals from the Lagoon Ojo de Liebre, B.C.S., Mexico. Allelic richness varied from 5 to 27 alleles per locus and the average expected heterozygosity was 0.76. Ten loci exhibited significant departure from Hardy–Weinberg equilibrium likely due to the presence of null alleles. Sixteen of these markers cross-amplified in closely related N. nodosus, while little or no amplification was observed in three Argopecten species.
Ecological Genomics Of Nematode Community Interactions: Model And Non-Model Approaches, Michael A. Herman, Joseph D. Coolon, Kenneth L. Jones, Timothy Todd
Ecological Genomics Of Nematode Community Interactions: Model And Non-Model Approaches, Michael A. Herman, Joseph D. Coolon, Kenneth L. Jones, Timothy Todd
School of Biological Sciences: Faculty Publications
The effects of human-induced environmental change are evident at multiple levels of biological organization. To date, most environmental change studies have focused on effects at the ecosystem, community, and organismal levels. However, the ultimate controls of biological responses are located in the genome. Thus, genetic and genomic studies of organismal responses to environmental changes are necessary. Recent advances in genome analysis now make such analyses possible. In this chapter we describe a research approach and program that can begin to span this gap by using genome-enabled approaches to characterize organismal changes and then employing a genetically tractable model organism to …
Letter From The Dean, Lalit Verma
Letter From The Dean, Lalit Verma
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
No abstract provided.
Pervasive Hitchhiking At Coding And Regulatory Sites In Humans, James J. Cai, J. Michael Macpherson, Guy Sella, Dmitri A. Petrov
Pervasive Hitchhiking At Coding And Regulatory Sites In Humans, James J. Cai, J. Michael Macpherson, Guy Sella, Dmitri A. Petrov
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Much effort and interest have focused on assessing the importance of natural selection, particularly positive natural selection, in shaping the human genome. Although scans for positive selection have identified candidate loci that may be associated with positive selection in humans, such scans do not indicate whether adaptation is frequent in general in humans. Studies based on the reasoning of the MacDonald–Kreitman test, which, in principle, can be used to evaluate the extent of positive selection, suggested that adaptation is detectable in the human genome but that it is less common than in Drosophila or Escherichia coli. Both positive and purifying …
Estrogenic Compounds Downstream From Three Small Cities In Eastern Nebraska: Occurrence And Biological Effect, Marlo K. Sellin, Daniel D. Snow, Debbie L. Akerly, Alan S. Kolok
Estrogenic Compounds Downstream From Three Small Cities In Eastern Nebraska: Occurrence And Biological Effect, Marlo K. Sellin, Daniel D. Snow, Debbie L. Akerly, Alan S. Kolok
Biology Faculty Publications
Recent studies have detected estrogenic compounds in surface waters in North America and Europe. Furthermore, the presence of estrogenic compounds in surface waters has been attributed, in some cases, to the discharge of wastewater treatment plant (WWTP) effluent. The primary objective of the current study was to determine if WWTP effluent contributes estrogens to the surface waters of Nebraska. A second objective of this study was to determine if estrogens were found in concentrations sufficient enough to manifest feminizing effects on fish. These objectives were satisfied by deploying polar organic chemical integrative samplers (POCIS) and caged fathead minnows at eight …
2009 Report : A Multi-Refuge Program To Evaluate The Effect Of Ungulate Browsing On Habitat, Richard B. Keigley, Jeffrey Warren, Wayne J. King
2009 Report : A Multi-Refuge Program To Evaluate The Effect Of Ungulate Browsing On Habitat, Richard B. Keigley, Jeffrey Warren, Wayne J. King
Aspen Bibliography
No abstract provided.
An Aspen Success Story : 2009 Aspen Monitoring Report, James L. Robertson
An Aspen Success Story : 2009 Aspen Monitoring Report, James L. Robertson
Aspen Bibliography
No abstract provided.
Multiple Colonisations Of The Western Indian Ocean By Pteropus Fruit Bats (Megachiroptera: Pteropodidae): The Furthest Islands Were Colonised First, John O'Brien, Carol Mariani, Link Olson, Amy L. Russell, Ludovic Say, Anne D. Yoder, Tom J. Hayden
Multiple Colonisations Of The Western Indian Ocean By Pteropus Fruit Bats (Megachiroptera: Pteropodidae): The Furthest Islands Were Colonised First, John O'Brien, Carol Mariani, Link Olson, Amy L. Russell, Ludovic Say, Anne D. Yoder, Tom J. Hayden
Amy L. Russell
The Evolution Of Flight In Insects: Insights From Mayflies And Dna, T. Heath Ogden
The Evolution Of Flight In Insects: Insights From Mayflies And Dna, T. Heath Ogden
T. Heath Ogden
No abstract provided.