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Full-Text Articles in Genetics and Genomics
Knockout Of Endospanin 1 Via Crispr In Zebrafish, Danio Rerio, Jared Kittinger
Knockout Of Endospanin 1 Via Crispr In Zebrafish, Danio Rerio, Jared Kittinger
Williams Honors College, Honors Research Projects
I made endospanin 1 knockout (KO) zebrafish to examine its effects on lipid and bone metabolism. Endospanin 1, or leptin receptor overlapping transcript (leprot), is a cytosolic protein linked to the protein hormone leptin that influences the trafficking of leptin receptors to the plasma membrane of cells. Genes for endospanin and tyrosinase (a pigmentation enzyme) were targeted via a microinjection of guide RNAs and CRISPR Cas9 into zebrafish embryos at 2-4 cell stages. I was able to disrupt the endospanin 1 gene (based upon the disruption of tyrosinase), but very few mutant zebrafish fully developed into adults. Only low KO …
Gene Expression Pattern Analysis Of Anterior Hox Genes During Zebrafish (Danio Rerio) Embryonic Development Reveals Divergent Expression Patterns From Other Teleosts, Adam Davis
Georgia Journal of Science
The regional identity of organs and organ systems along the anterior-posterior axis during embryonic development is patterned, in part, by Hox genes, which encode transcription factor proteins that activate or repress the expression of downstream target genes. Divergent nested Hox gene expression patterns may have had a role in facilitating morphological divergence of structures, such as the pharyngeal jaw apparatus, among evolutionarily divergent teleost fishes. Recent studies from several evolutionarily divergent teleosts, such as the Japanese Medaka (Oryzias latipes) and the Nile Tilapia (Oreochromis niloticus), have shown the presence of divergent expression patterns of several Hox …
The Time Progression Of Bub1 And Cdc2 Activation In Zebrafish (Danio Rerio) During Egg Maturation, Julie Swinson
The Time Progression Of Bub1 And Cdc2 Activation In Zebrafish (Danio Rerio) During Egg Maturation, Julie Swinson
Summer Research
The genes bub1 and cdc20 are involved in regulation of meiotic arrest during maturation of the oocyte (immature egg). Meiotic arrest is important in oogenesis, the development of fertilizable eggs, and allows for identification of any DNA mutations or chromosome assembly errors, thus preventing damaged eggs from being ovulated. This study has examined the expression of spindle assembly checkpoint genes bub1 and cdc20. Oocytes were treated with DHP (hormone to induce maturation) or by nocodazole, a spindle destabilizing agent, and relative amounts of mRNA were measured using RT-PCR. These experiments will establish a timeline of bub1 and cdc20 activation …
From Dna To Protein: A Study Of Genomic Instability Candidate Genes During Zebrafish Development, Kristine Griffett
From Dna To Protein: A Study Of Genomic Instability Candidate Genes During Zebrafish Development, Kristine Griffett
USF Tampa Graduate Theses and Dissertations
The zebrafish, Danio rerio, is a type of freshwater minnow often used to model human diseases including cancer, anxiety and aging diseases. The overall biology of zebrafish is strikingly similar to that of humans, allowing these fish to be used for drug discovery and toxicology studies for preclinical trials. In this study, zebrafish embryos were used to identify and characterize several candidate genes within two known regions of genomic instability on chromosome 18 and chromosome 4. This fish that were used in this study had been previously classified as genomic instability (gin) mutants due to increased incidence of somatic mutation …