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Full-Text Articles in Genetics and Genomics
Quantitative Analysis Of The Thrbcrm1-Centered Gene Regulatory Network, Benjamin Souferi, Mark M. Emerson
Quantitative Analysis Of The Thrbcrm1-Centered Gene Regulatory Network, Benjamin Souferi, Mark M. Emerson
Publications and Research
Enhancer activity is determined by both the activity and occupancy of transcription factors as well as the specific sequences they bind. Experimental investigation of this dynamic requires the ability to manipulate components of the system, ideally in as close to an in vivo context as possible. Here we use electroporation of plasmid reporters to define critical parameters of a specific cis-regulatory element, ThrbCRM1, during retinal development. ThrbCRM1 is associated with cone photoreceptor genesis and activated in a subset of developing retinal cells that co-express the Otx2 and Onecut1 (OC1) transcription factors. Variation of reporter plasmid concentration was used to generate …