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Full-Text Articles in Genetics and Genomics

Expression Of Lysine-Mediated Neuropeptide Hormones Controlling Satiety And Appetite In Broiler Chickens, Collins N. Khwatenge, Boniface M. Kimathi, Thyneice Taylor-Bowden, Samuel N. Nahashon Dec 2019

Expression Of Lysine-Mediated Neuropeptide Hormones Controlling Satiety And Appetite In Broiler Chickens, Collins N. Khwatenge, Boniface M. Kimathi, Thyneice Taylor-Bowden, Samuel N. Nahashon

Agricultural and Environmental Sciences Faculty Research

Lysine is the second most limiting amino acid after methionine and is considered the most limiting amino acid for growth in poultry. Lysine requirement for broiler chickens has changed over the years. Leptin and adiponectin represent 2 adipokines that mediate metabolism by eliciting satiety effects whereas ghrelin peptide hormone influences appetite. We hypothesize that this affects growth performance of chicks. This study evaluates the effect of varying dietary lysine homeostasis on performance of broiler chickens through satiety- and appetite-mediating hormones. In 3 replications, 270 one-day-old chicks were reared for 8 wk feeding on diets comprising 0.85, 1.14, and 1.42% lysine …


Use Of Probiotic Bacterial Strains And Cell Extracts To Inhibit Acidosis And Liver Abscesses In Cattle, Samodha Charaka Fernand Sep 2019

Use Of Probiotic Bacterial Strains And Cell Extracts To Inhibit Acidosis And Liver Abscesses In Cattle, Samodha Charaka Fernand

Department of Animal Science: Faculty Publications

Disclosed are methods of using probiotic bacterial strains Aneurinibacillus migulanus, Aneurinibacillus aneurinilyti cus and / or extracts thereof for inhibiting the growth of Streptococcus bovis , Fusobacterium necrophorum , and Acranobacterium ( Actinomyces ) pyogenes . These bacterial strains and / or their extracts can be used in cattle to treat acidosis and / or liver abscesses , as well as inhibit foot rot, among other infections and conditions caused by Strepto coccus bovis and / or Fusobacterium necrophorum and / or Acranobacterium (Actinomyces) pyogenes. Compositions for use in these methods are also provided.


Postnatal Nutrient Repartitioning Due To Adaptive Developmental Programming, Robert J. Posont, Dustin T. Yates Jul 2019

Postnatal Nutrient Repartitioning Due To Adaptive Developmental Programming, Robert J. Posont, Dustin T. Yates

Department of Animal Science: Faculty Publications

The consequences of prenatal stress on lifelong metabolic function and health was first proposed by David Barker and Nicholas Hales with the publication of their Thrifty Phenotype Hypothesis in the early 1990s.1,2 Subsequent studies in humans and animals have further demonstrated that stress-induced adaptive fetal programming leads to tissue-specific changes in metabolic function and growth capacity.3,4 Developmental adaptations to the intrauterine nutrient restriction that accompanies most maternofetal stressors target regulatory pathways for nutrient utilization in non-essential tissues such as skeletal muscle.4-6 This aids intrauterine survival by re-appropriating nutrients to support neural, cardiac, and endocrine tissue function but reduces metabolic efficiency …


Beef Production Systems In The Nebraska Sandhills, Mckay Erickson Jun 2019

Beef Production Systems In The Nebraska Sandhills, Mckay Erickson

Department of Animal Science: Dissertations, Theses, and Student Research

Four studies were conducted to determine the effectiveness of improving production systems of beef cattle in the Sandhills region of Nebraska. These studies were to determine impacts of modified estrus synchronization protocols, genomic testing heifer calves for longevity, and evaluation of 2 differing calving systems (March or May) for improving biological outcome and improved production. Experiment 1 utilized 180 yearling heifers to determine the effectiveness of a second dose of prostaglandin F(PGF) with those females not expressing estrus after an initial 14 d MGA-PG estrus synchronization protocol. The treatment of PGF did increase estrus expression, but did not …


Utilizing An Electronic Feeder To Measure Mineral And Energy Supplement Intake In Beef Heifers Grazing Native Range, Kacie L. Mccarthy, Sarah R. Underdahl, Michael Undi, Stephanie Becker, Carl R. Dahlen Jun 2019

Utilizing An Electronic Feeder To Measure Mineral And Energy Supplement Intake In Beef Heifers Grazing Native Range, Kacie L. Mccarthy, Sarah R. Underdahl, Michael Undi, Stephanie Becker, Carl R. Dahlen

Department of Animal Science: Faculty Publications

Introduction

Grasslands in the Northern Plains provide the primary forage source for ruminants throughout much of the year (Schauer et al., 2004). Supplementation practices are often necessary to maintain production and offset forage nutritive decline throughout the grazing season (Schauer et al., 2004; Cline et al., 2009). Typically, to maintain a targeted production level, energy and protein supplementations are used for grazing livestock (Caton and Dhuyvetter, 1997). For developing heifers consuming low-quality forages, inclusion of energy ingredients into supplements may be beneficial for growth and reproductive performance (Schillo et al., 1992; Ciccioli et al., 2005; Cappellozza et al., 2014). In …


Effect Of Cooled Perches On The Efficacy Of An Induced Molt In White Leghorn Laying Hens Previously Exposed To Heat Stress, J Y. Hu, P Y. Hester, Y Xiong, R S. Gates, M M. Makagon, H W. Cheng May 2019

Effect Of Cooled Perches On The Efficacy Of An Induced Molt In White Leghorn Laying Hens Previously Exposed To Heat Stress, J Y. Hu, P Y. Hester, Y Xiong, R S. Gates, M M. Makagon, H W. Cheng

Department of Animal Science: Faculty Publications

This study examined the effect of water chilled perches on hen production and physiological responses to induced molt during elevated temperatures. A total of 288White Leghorns at 82 wk of age were housed in 36 cages of 6 banks. Each bank was assigned to 1 of 3 treatments: cooled perches, air perches, and no perches. The hens were subjected to 2 heat episodes during their first laying cycle at week 21 to 35 and week 73 to 80, respectively. The hens were subjected to a 28 D nonfasted molting regimen starting at 85 wk of age. Cyclic heat of 32◦C …


Hiding In The Lianas Of The Tree Of Life: Molecular Phylogenetics And Species Delimitation Reveal Considerable Cryptic Diversity Of New World Vine Snakes, Robert C. Jadin, Christopher Blair, Michael J. Jowers, Anthony Carmona, John C. Murphy May 2019

Hiding In The Lianas Of The Tree Of Life: Molecular Phylogenetics And Species Delimitation Reveal Considerable Cryptic Diversity Of New World Vine Snakes, Robert C. Jadin, Christopher Blair, Michael J. Jowers, Anthony Carmona, John C. Murphy

Publications and Research

The Brown Vine Snake, Oxybelis aeneus, is considered a single species despite the fact its distribution covers an estimated 10% of the Earth’s land surface, inhabiting a variety of ecosystems throughout North, Central, and South America and is distributed across numerous biogeographic barriers. Here we assemble a multilocus molecular dataset (i.e. cyt b, ND4, cmos, PRLR) derived from Middle American populations to examine for the first time the evolutionary history of Oxybelis and test for evidence of cryptic lineages using Bayesian and maximum likelihood criteria. Our divergence time estimates suggest that Oxybelis diverged from its sister genus, Leptophis …


Substantial Red Wolf Genetic Ancestry Persists In Wild Canids Of Southwestern Louisiana, Jennifer R. Adams, John J. Cox, Lisette P. Waits Mar 2019

Substantial Red Wolf Genetic Ancestry Persists In Wild Canids Of Southwestern Louisiana, Jennifer R. Adams, John J. Cox, Lisette P. Waits

Forestry and Natural Resources Faculty Publications

Concerns over red wolf (Canis rufus) extinction caused by hybridization with coyotes (C. latrans) led to the capture and removal of remnant wild wolves from southwestern Louisiana and southeastern Texas, United States, during the 1970s. Here we show that despite decades of unmitigated hybridization, and declaration of endangered red wolves as functionally extinct in the wild, red wolf mitochondrial or nuclear DNA ancestry persists in ∼55% of contemporary wild canids sampled in southwestern Louisiana. Surprisingly, one individual had 78–100% red wolf ancestry, which is within the range for 75% red wolf, red wolf backcross, or putative …


Crispr-Mediated Gene Editing: Scientific And Ethical Issues, Jarrod Bailey Mar 2019

Crispr-Mediated Gene Editing: Scientific And Ethical Issues, Jarrod Bailey

Genetics Collection

There remains substantial evidence to warrant great concern over the poor efficiency and specificity of clustered regularly interspaced short palindromic repeats (CRISPR)-mediated genetic modification (GM), despite relatively minor improvements compared to other GM methods. These issues cause persistent, adverse, ethical, and scientific consequences for GM animals, which may never be sufficiently resolvable.


Maternal Inflammation At Midgestation Impairs Subsequent Fetal Myoblast Function And Skeletal Muscle Growth In Rats, Resulting In Intrauterine Growth Restriction At Term, Caitlin N. Cadaret, Robert J. Posont, Kristin A. Beede, Hannah E. Riley, John Dustin Loy, Dustin T. Yates Feb 2019

Maternal Inflammation At Midgestation Impairs Subsequent Fetal Myoblast Function And Skeletal Muscle Growth In Rats, Resulting In Intrauterine Growth Restriction At Term, Caitlin N. Cadaret, Robert J. Posont, Kristin A. Beede, Hannah E. Riley, John Dustin Loy, Dustin T. Yates

Department of Animal Science: Faculty Publications

Maternal inflammation induces intrauterine growth restriction (MI-IUGR) of the fetus, which compromises metabolic health in human offspring and reduces value in livestock. The objective of this study was to determine the effect of maternal inflammation at midgestation on fetal skeletal muscle growth and myoblast profiles at term. Pregnant Sprague-Dawley rats were injected daily with bacterial endotoxin (MI-IUGR) or saline (controls) from the 9th to the 11th day of gestational age (dGA; term = 21 dGA). At necropsy on dGA 20, average fetal mass and upper hindlimb cross-sectional areas were reduced (P < 0.05) in MI-IUGR fetuses compared with controls. MyoD+ and myf5+ myoblasts were less abundant (P < 0.05), and myogenin+ myoblasts were more abundant (P < 0.05) in MI-IUGR hindlimb skeletal muscle compared with controls, indicating precocious myoblast differentiation. Type I and Type II hindlimb muscle fibers were smaller (P < 0.05) in MI-IUGR fetuses than in controls, but fiber type proportions did not differ between experimental groups. Fetal blood plasma TNFα concentrations were below detectable amounts in both experimental groups, but skeletal muscle gene expression for the cytokine receptors TNFR1, IL6R, and FN14 was greater (P < 0.05) in MI-IUGR fetuses than controls, perhaps indicating enhanced sensitivity to these cytokines. Maternal blood glucose concentrations at term did not differ between experimental groups, but MI-IUGR fetal blood contained less (P < 0.05) glucose, cholesterol, and triglycerides. Fetal-to-maternal blood glucose ratios were also reduced (P < 0.05), which is indicative of placental insufficiency. Indicators of protein catabolism, including blood plasma urea nitrogen and creatine kinase, were greater (P < 0.05) in MI-IUGR fetuses than in controls. From these findings, we conclude that maternal inflammation at midgestation causes muscle-centric fetal programming that impairs myoblast function, increases protein catabolism, and reduces skeletal muscle growth near term. Fetal muscle sensitivity to inflammatory cytokines appeared to be enhanced after maternal inflammation, which may represent a mechanistic target for improving these outcomes in MI-IUGR fetuses.


Blood Parasites Infecting The Hoatzin (Opisthocomus Hoazin), A Unique Neotropical Folivorous Bird, M. Andreína Pacheco, M. Alexandra García-Amado, Jaime Manzano, Nubia E. Matta, Ananias A. Escalante Feb 2019

Blood Parasites Infecting The Hoatzin (Opisthocomus Hoazin), A Unique Neotropical Folivorous Bird, M. Andreína Pacheco, M. Alexandra García-Amado, Jaime Manzano, Nubia E. Matta, Ananias A. Escalante

Harold W. Manter Laboratory: Library Materials

The Hoatzin (Opisthocomus hoazin) is the only extant member of the order Opisthocomiformes. This unique South American bird lives in the riparian lowland vegetation characteristic of the Amazon and Orinoco basins. Hoatzins nest in communal social units close to water bodies; they are strictly folivores being the only bird with pregastric fermentation in the crop. Because of the complex logistics involved in capturing this bird, there is a knowledge gap on its parasites. This study documents two distant lineages of haemosporidian parasites (Plasmodium spp.) in a juvenile and two adults sampled in the Cojedes state, Venezuela. Although …


Effect Of Cooled Perches On Performance, Plumage Condition, And Foot Health Of Caged White Leghorn Hens Exposed To Cyclic Heat, J Y. Hu, P Y. Hester, M M. Makagon, Y Xiong, R S. Gates, H W. Cheng Jan 2019

Effect Of Cooled Perches On Performance, Plumage Condition, And Foot Health Of Caged White Leghorn Hens Exposed To Cyclic Heat, J Y. Hu, P Y. Hester, M M. Makagon, Y Xiong, R S. Gates, H W. Cheng

Department of Animal Science: Faculty Publications

We examined the effects of water-chilled perches as cooling devices on hen performance during 2 summers using daily cyclic heat. White Leghorns, 17 wk of age, were assigned to 36 cages arranged into 6 banks. The banks were assigned to cooled perches, air perches, and no perches resulting in 2 replicate banks and 12 cages per treatment. Chilled water (10C) was circulated through the cooled perches during heat episodes. Daily cyclic heat of 35C was applied from 0600 to1800 h with a lowering of temperature to 28C from 1800 to 0600 h during the …


Biotechnological Application In Aquaculture And Its Sustainability Constraints, Olaganathan Rajee, Tang Kar Mun Alicia Jan 2019

Biotechnological Application In Aquaculture And Its Sustainability Constraints, Olaganathan Rajee, Tang Kar Mun Alicia

Publications

The valuable nutritional and biochemical properties have made fisheries products one of the most vital high-quality protein sources for human consumption. Aquaculture has become the great alternative to substitute wild catches when the yield from fishing are no longer sufficient to sustain the massive food demand of the human population which is constantly burgeoning. However, aquaculture requires multidisciplinary approaches with holistic and environmental-friendly management measures to ensure its long term success and sustainability. Biotechnological applications have enhanced the effectiveness and cost-efficiencies of aquaculture by augmenting the productivity of aquaculture to meet global needs. Despite the benefits, the biotechnological application in …


Livestock Animals To Study Infertility In Women, Mohamed A. Abedal-Majed, Andrea S. Cupp Jan 2019

Livestock Animals To Study Infertility In Women, Mohamed A. Abedal-Majed, Andrea S. Cupp

Department of Animal Science: Faculty Publications

  • Anovulation is a major cause of infertility in mammalian females.

  • Ovarian research in humans is challenging due to the shortage of “normal tissue” that can be obtained from women that are trying to get pregnant.

  • Livestock animals provide valuable resources to investigate the optimal intra-follicular environment required to facilitate folliculogenesis and translate to humans.

  • Different livestock models may be more useful depending on the specific biological process studied.


Evaluation Of The Effects Of Biochar On Diet Digestibility And Methane Production From Growing And Finishing Steers, Thomas Winders, Melissa L. Jolly, Hannah C. Wilson, James C. Macdonald, Galen E. Erickson, Andrea K. Watson Jan 2019

Evaluation Of The Effects Of Biochar On Diet Digestibility And Methane Production From Growing And Finishing Steers, Thomas Winders, Melissa L. Jolly, Hannah C. Wilson, James C. Macdonald, Galen E. Erickson, Andrea K. Watson

Department of Animal Science: Faculty Publications

The objectives of these studies were to evaluate the effects of biochar (0%, 0.8%, or 3% of diet dry matter) on diet digestibility and methane and carbon dioxide production from cattle on growing and finishing diets. The growing diet consisted of 21% brome hay, 20% wheat straw, 30% corn silage, 22% wet distillers grains plus solubles, and 7% supplement. The finishing diet consisted of 53% dry-rolled corn, 15% corn silage, 25% wet distillers grains plus solubles, and 7% supplement. In both trials biochar replaced fine ground corn in the supplement. Six crossbred steers (initial body weight [BW] 529 kg; SD …


The Impact Of Heat Load On Cattle, Angela M. Lees, Veerasamy Sejian, Andrea L. Wallage, Cameron C. Steel, Terry L. Mader, Jarrod C. Lees, John B. Gaughan Jan 2019

The Impact Of Heat Load On Cattle, Angela M. Lees, Veerasamy Sejian, Andrea L. Wallage, Cameron C. Steel, Terry L. Mader, Jarrod C. Lees, John B. Gaughan

Department of Animal Science: Faculty Publications

Heat stress and cold stress have a negative influence on cattle welfare and productivity. There have been some studies investigating the influence of cold stress on cattle, however the emphasis within this review is the influence of heat stress on cattle. The impact of hot weather on cattle is of increasing importance due to the changing global environment. Heat stress is a worldwide phenomenon that is associated with reduced animal productivity and welfare, particularly during the summer months. Animal responses to their thermal environment are extremely varied, however, it is clear that the thermal environment influences the health, productivity, and …


Evaluation Of Genotype Quality Parameters For Sowpro90, A New Genotyping Array For Swine, Hiruni R. Wijesena, Gary A. Rohrer, Dan J. Nonneman, Brittney N. Keel, Jessica L. Petersen, Stephen D. Kachman, Daniel C. Ciobanu Jan 2019

Evaluation Of Genotype Quality Parameters For Sowpro90, A New Genotyping Array For Swine, Hiruni R. Wijesena, Gary A. Rohrer, Dan J. Nonneman, Brittney N. Keel, Jessica L. Petersen, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Understanding early predictors of sow fertility has the potential to improve genomic predictions. A custom SNP array (SowPro90 produced by Affymetrix) was developed to include genetic variants overlapping quantitative trait loci for age at puberty, one of the earliest indicators of sow fertility, as well as variants related to innate and adaptive immunity. The polymorphisms included in the custom genotyping array were identified using multiple genomic approaches including deep genomic and transcriptomic sequencing and genome-wide associations. Animals from research and commercial populations (n = 2,586) were genotyped for 103,476 SNPs included in SowPro90. To assess the quality …


Coding Sequences Of Sarcoplasmic Reticulum Calcium Atpase Regulatory Peptides And Expression Of Calcium Regulatory Genes In Recurrent Exertional Rhabdomyolysis, Stephanie J. Valberg, Kaitlin Soave, Zoe J. Williams, Sudeep Perumbakkam, Melissa Schott, Carrie J. Finno, Jessica L. Petersen, Clara Fenger, Joseph M. Autry, David D. Thomas Jan 2019

Coding Sequences Of Sarcoplasmic Reticulum Calcium Atpase Regulatory Peptides And Expression Of Calcium Regulatory Genes In Recurrent Exertional Rhabdomyolysis, Stephanie J. Valberg, Kaitlin Soave, Zoe J. Williams, Sudeep Perumbakkam, Melissa Schott, Carrie J. Finno, Jessica L. Petersen, Clara Fenger, Joseph M. Autry, David D. Thomas

Department of Animal Science: Faculty Publications

Background: Sarcolipin (SLN), myoregulin (MRLN), and dwarf open reading frame (DWORF) are transmembrane regulators of the sarcoplasmic reticulum calcium transporting ATPase (SERCA) that we hypothesized played a role in recurrent exertional rhabdomyolysis (RER).

Objectives: Compare coding sequences of SLN, MRLN, DWORF across species and between RER and control horses. Compare expression of muscle Ca2+ regulatory genes between RER and control horses.

Animals: Twenty Thoroughbreds (TB), 5 Standardbreds (STD), 6 Quarter Horses (QH) with RER and 39 breed-matched controls.

Methods: Sanger sequencing of SERCA regulatory genes with comparison of amino acid (AA) …


Maternal Age Influences The Number Of Primordial Follicles In The Ovaries Of Yearling Angus Heifers, Sarah C. Tenley, Renata Spuri Gomes, Shelby L. Rosasco, Emmalee J. Northrop, Jerica J. J. Rich, Anthony K. Mcneel, Adam F. Summers, Jeremy R. Miles, Chadwick C. Chase Jr., Clay A. Lents, George A. Perry, Jennifer R. Wood, Andrea S. Cupp, Robert A. Cushman Jan 2019

Maternal Age Influences The Number Of Primordial Follicles In The Ovaries Of Yearling Angus Heifers, Sarah C. Tenley, Renata Spuri Gomes, Shelby L. Rosasco, Emmalee J. Northrop, Jerica J. J. Rich, Anthony K. Mcneel, Adam F. Summers, Jeremy R. Miles, Chadwick C. Chase Jr., Clay A. Lents, George A. Perry, Jennifer R. Wood, Andrea S. Cupp, Robert A. Cushman

Department of Animal Science: Faculty Publications

The number of antral follicles detectable by ultrasonography in heifers is influenced by age of the dam, because daughters of primiparous cows have fewer antral follicles than daughters of mature cows. We, therefore, hypothesized that heifers with primiparous dams would have fewer primordial follicles in their ovaries than heifers born to mature (4+ y) cows. Angus heifers (n=464) were submitted for ultrasonographic evaluation of antral follicle number at 325, 355, and 385 d of age. Ovaries were collected from a random subset of heifers (n=79) and processed for histological evaluation to determine number of primordial follicles. A greater percentage of …


Increasing The Concentration Of Linolenic Acid In Diets Fed To Jersey Cows In Late Lactation Does Not Affect Methane Production, J. V. Judy, G. C. Bachman, T. M. Brown-Brandl, S. C. Fernando, K. E. Hales, K. J. Harvatine, P. S. Miller, P. J. Kononoff Jan 2019

Increasing The Concentration Of Linolenic Acid In Diets Fed To Jersey Cows In Late Lactation Does Not Affect Methane Production, J. V. Judy, G. C. Bachman, T. M. Brown-Brandl, S. C. Fernando, K. E. Hales, K. J. Harvatine, P. S. Miller, P. J. Kononoff

Department of Animal Science: Faculty Publications

Although the inclusion of fat has reduced methane production in ruminants, relatively little research has been conducted comparing the effects of source and profile of fatty acids on methane production in lactating dairy cows. A study using 8 multiparous (325 ± 17 DIM; mean ± SD) lactating Jersey cows was conducted to determine effects of feeding canola meal and lard versus extruded byproduct containing flaxseed as a high-C18:3 fat source on methane production and diet digestibility in late-lactation dairy cows. A crossover design with 32-d periods (28-d adaptation and 4-d collections) was used to compare 2 different fat sources. Diets …


Real Supermodels Wear Wool: Summarizing The Impact Of The Pregnant Sheep As An Animal Model For Adaptive Fetal Programming, Kristin A. Beede, Sean W. Limesand, Jessica L. Petersen, Dustin T. Yates Jan 2019

Real Supermodels Wear Wool: Summarizing The Impact Of The Pregnant Sheep As An Animal Model For Adaptive Fetal Programming, Kristin A. Beede, Sean W. Limesand, Jessica L. Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

• Intrauterine growth restriction (IUGR) continues to be a global epidemic that is associated with high early-life mortality rates and greater risk for developing metabolic disorders that lower length and quality of life in affected individuals.

• Fetal programming of muscle growth and metabolic function associated with IUGR is often comparable among nonlitter bearing mammalian species, which allows much of the information learned in domestic animal models to be applicable to humans (and other animals).

• Recent studies in sheep models of IUGR have begun to uncover the molecular mechanisms linking adaptive fetal programming and metabolic dysfunction.

• Targets of …


The Heritability Of Pampiniform Plexus Vessel Size And Varicocoele In Boars, Tasha R. Gruhot, Lea A. Rempel, Matthew L. Spangler, Stephen D. Kachman, Benny E. Mote Jan 2019

The Heritability Of Pampiniform Plexus Vessel Size And Varicocoele In Boars, Tasha R. Gruhot, Lea A. Rempel, Matthew L. Spangler, Stephen D. Kachman, Benny E. Mote

Department of Animal Science: Faculty Publications

Ultrasonography was used to capture a coronal–sagittal image of the veins of the pampiniform plexus (PP) and the testicular artery of 327 maternal‐line boars at approximately 6 months of age at the University of Nebraska—Lincoln. Varicocoele was diagnosed by two methods. Method 1 diagnosed varicocoele when the average vessel area on one side of the scrotum was 1.5 times larger than the average vessel area on the other side of the scrotum. Method 2 diagnosed varicocoele when the average vessel area on one side of the scrotum of a boar was 1.5 times larger than the average vessel on the …


Effect Of Harvest Method And Ammoniation Of Baled Corn Residue On Intake And Digestibility In Lambs, Ashley C. Conway, Tasha M. King, Melissa L. Jolly, J. C. Macdonald, Terry J. Klopfenstein, Mary E. Drewnoski Jan 2019

Effect Of Harvest Method And Ammoniation Of Baled Corn Residue On Intake And Digestibility In Lambs, Ashley C. Conway, Tasha M. King, Melissa L. Jolly, J. C. Macdonald, Terry J. Klopfenstein, Mary E. Drewnoski

Department of Animal Science: Faculty Publications

To determine the effect of harvest method and ammoniation on both in vivo and in vitro digestibility of corn residue, six corn residue treatments consisting of three different harvest methods either with or without anhydrous ammonia chemical treatment (5.5% of dry matter [DM]) were evaluated. The harvest methods included conventional rake-and-bale (CONV) and New Holland Cornrower with eight rows (8ROW) or two rows (2ROW) of corn stalks chopped into the windrow containing the tailings (leaf, husk, and upper stem) from eight rows of harvested corn (ammoniated bales of each harvest method resulted in treatments COVAM, 8RAM, and 2RAM). Nine crossbred …


Effect Of Environmental Temperature And Β-Adrenergic Agonist Supplementation On Rumen Volatile Fatty Acid Production In Sheep, Erin M. Duffy, Hannah C. Hamilton, Ty B. Schmidt, Dustin T. Yates, Jessica Lynn Petersen Jan 2019

Effect Of Environmental Temperature And Β-Adrenergic Agonist Supplementation On Rumen Volatile Fatty Acid Production In Sheep, Erin M. Duffy, Hannah C. Hamilton, Ty B. Schmidt, Dustin T. Yates, Jessica Lynn Petersen

Department of Animal Science: Faculty Publications

Many environmental and dietary factors affect ruminant livestock performance (Ames, 1980; Carberry et al., 2012). Energy used by ruminants is mainly derived from volatile fatty acids (VFA), including acetate, propionate, and butyrate, which are produced by bacterial fermentation of carbohydrates (Barcroft et al., 1944). With the importance of VFA production in ruminants (Bergman, 1990), it is essential to understand how various conditions livestock may be subjected to, such as ambient conditions and diet, can affect VFA production.

With the profound role of VFA production in energy status of the ruminant, and given that heat stress (HS) events can affect ruminal …


Ecophysiological Determinants Of Sexual Size Dimorphism: Integrating Growth Trajectories, Environmental Conditions, And Metabolic Rates, Marie-Claire Chelini, John P. Delong, Eileen A. Hebets Jan 2019

Ecophysiological Determinants Of Sexual Size Dimorphism: Integrating Growth Trajectories, Environmental Conditions, And Metabolic Rates, Marie-Claire Chelini, John P. Delong, Eileen A. Hebets

Eileen Hebets Publications

Sexual size dimorphism (SSD) often results in dramatic differences in body size between females and males. Despite its ecological importance, little is known about the relationship between developmental, physiological, and energetic mechanisms underlying SSD. We take an integrative approach to understand the relationship between developmental trajectories, metabolism, and environmental conditions resulting in extreme female-biased SSD in the crab spider Mecaphesa celer (Thomisidae). We tested for sexual differences in growth trajectories, as well as in the energetics of growth, hypothesizing that female M. celer have lower metabolic rates than males or higher energy assimilation. We also hypothesized that the environment in …


Metabolomics Profile And Targeted Lipidomics In Multiple Tissues Associated With Feed Efficiency In Beef Steers, Virginia M. Artegoitia, Andrew P. Foote, Ronald M. Lewis, Harvey C. Freetly Jan 2019

Metabolomics Profile And Targeted Lipidomics In Multiple Tissues Associated With Feed Efficiency In Beef Steers, Virginia M. Artegoitia, Andrew P. Foote, Ronald M. Lewis, Harvey C. Freetly

Department of Animal Science: Faculty Publications

A study of multiple tissues was conducted to identify potential metabolic differences in cattle differing in feed efficiency. Individual feed intake and body weight was measured on 144 steers during 105 days on a high-concentrate ration. Steers were selected according to differences in average daily gain (ADG) with those with the greatest ADG (n = 8; 1.96 ± 0.02 kg/day) and least ADG (n = 8; 1.57 ± 0.02 kg/day), whose dry matter intake was within 0.32 SD of the mean intake (10.10 ± 0.05 kg/day). Duodenum, liver, adipose, and longissimus-dorsi were collected at slaughter, and metabolomics profiles …


Contemporary Sexual Selection Does Not Explain Variation In Male Display Traits Among Populations, J. Colton Watts, Allissa Flynn, Brigitte Tenhumberg, Eileen Hebets Jan 2019

Contemporary Sexual Selection Does Not Explain Variation In Male Display Traits Among Populations, J. Colton Watts, Allissa Flynn, Brigitte Tenhumberg, Eileen Hebets

Brigitte Tenhumberg Papers

Sexual selection is widely hypothesized to facilitate the evolution of reproductive isolation through divergence in sexual traits and sexual trait preferences among populations. However, direct evidence of divergent sexual selection causing intraspecific trait divergence remains limited. Using the wolf spider Schizocosa crassipes, we characterized patterns of female mate choice within and among geographic locations and related those patterns to geographic variation in male display traits to test whether divergent sexual selection caused by mate choice explains intraspecific trait variation. We found evidence of phenotypic selection on male behavior arising from female mate choice, but no evidence that selection varied …


Exploration Of Sonic Hedgehog Gene Expression In Fathead Minnows (Pimephales Promelas), Brooke Renee Greiner Jan 2019

Exploration Of Sonic Hedgehog Gene Expression In Fathead Minnows (Pimephales Promelas), Brooke Renee Greiner

Undergraduate Honors Theses

Pollutants, as a result of wastewater treatments, have been shown to have negative impacts on aquatic ecosystems. To better understand the possible consequences caused by effluents on ecosystems, it is important to examine ecotoxicology data. One of the most commonly used species for water quality testing is the fathead minnow, Pimephales promelas. Ecotoxicology can then be taken one step further to understand the effects of pollutants on a molecular level. Previous research had identified effluents as causes for abnormal minnow fin morphology. In order to collect additional data on development, tanks with fathead minnows were placed at the Charleston …


Maternal Inflammation At 0.7 Gestation In Ewes Leads To Intrauterine Growth Restriction And Impaired Glucose Metabolism In Offspring At 30 D Of Age, Robert J. Posont, Caitlin N. Cadaret, Kristin A. Beede, Joslyn K. Beard, Rebecca M. Swanson, Rachel L. Gibbs, Jessica L. Petersen, Dustin T. Yates Jan 2019

Maternal Inflammation At 0.7 Gestation In Ewes Leads To Intrauterine Growth Restriction And Impaired Glucose Metabolism In Offspring At 30 D Of Age, Robert J. Posont, Caitlin N. Cadaret, Kristin A. Beede, Joslyn K. Beard, Rebecca M. Swanson, Rachel L. Gibbs, Jessica L. Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

Fetal programming associated with intrauterine growth restriction (IUGR) leads to lifelong deficits in growth and metabolic function (Hales and Barker, 2013). IUGR arises when fetuses respond to poor in utero conditions by developing adaptations that repartition nutrients to critical tissues and away from skeletal muscle (Yates et al., 2012, 2018). This fetal programming is beneficial in utero but leads to persistent reductions in muscle mass and glucose homeostasis in offspring (DeFronzo et al., 1981). Recent studies by our laboratory in sheep and rats demonstrate that maternal inflammation during gestation induces fetal inflammatory adaptations that impair growth and disrupt muscle glucose …


Body Composition Estimated By Bioelectrical Impedance Analyses Is Diminished By Prenatal Stress In Neonatal Lambs And By Heat Stress In Feedlot Wethers, Rachel L. Gibbs, Caitlin N. Cadaret, Rebecca M. Swanson, Kristin A. Beede, Robert J. Posont, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates Jan 2019

Body Composition Estimated By Bioelectrical Impedance Analyses Is Diminished By Prenatal Stress In Neonatal Lambs And By Heat Stress In Feedlot Wethers, Rachel L. Gibbs, Caitlin N. Cadaret, Rebecca M. Swanson, Kristin A. Beede, Robert J. Posont, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

Body composition correlates to carcass value in livestock, which makes the ability to accurately estimate body composition in the live animal beneficial (Berg and Marchello, 1994). Bioelectrical impedance analysis (BIA) is a clinical tool used to assess body composition in humans (Lukaski et al., 1985), but its use in livestock has been minimal. Lean and fat content contribute to profitability for livestock producers, and poor body composition can be caused by stress that occurs either during in utero development (De Blasio et al., 2007) or during postnatal growth (Boyd et al., 2015). Maternal hyperthermia-induced placental insufficiency (Brown et al., 2015) …