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Full-Text Articles in Forest Sciences
The Effects Of Zinc Oxide Nanoparticles Loaded With Iaa And Iba On In Vitro Rooting Ofapple Microcuttings, Shabnam Alizadeh, Hati̇ce Dumanoğlu
The Effects Of Zinc Oxide Nanoparticles Loaded With Iaa And Iba On In Vitro Rooting Ofapple Microcuttings, Shabnam Alizadeh, Hati̇ce Dumanoğlu
Turkish Journal of Agriculture and Forestry
In the micropropagation of difficult-to-root plants, the low rates of rooting cause high economic losses. The local apple (Malus domestica Borkh.) cultivar #67, which is highly resistant to fire blight disease, has been shown to have low rooting success. Nanoparticles loaded with auxins could increase the rooting success with their physicochemical properties. To increase rooting percentage, the effects of zinc oxide nanoparticles (nZnO), nZnO loaded with indole-3-acetic acid (IAA-nZnO) and indole-3-butyric acid (IBA-nZnO) at different concentrations (1.0, 2.0, 3.0, 4.0, 5.0, and 6.0 mg L-1) on in vitro rooting of microcuttings were investigated. The concentrations of auxin carried by the …
Screening Of Factors Affecting Somatic Callusing And Embryo Induction In Allium Cepa L.Through Plackett?Burman Methodology, Kourosh Keighobadi, Maryam Golabadi, Mahdi Khozaei, Abdol Majid Rezai
Screening Of Factors Affecting Somatic Callusing And Embryo Induction In Allium Cepa L.Through Plackett?Burman Methodology, Kourosh Keighobadi, Maryam Golabadi, Mahdi Khozaei, Abdol Majid Rezai
Turkish Journal of Agriculture and Forestry
Induction of embryonic callus through available asexual explant culture is applied in the protection and maintenance of cytoplasmic-male sterility and in the maintenance of other lines of onion (Allium cepa L.). Usage of the Plackett?Burman (PB) experimental design has been suggested as a screening method for many variables that influence the desired induction. In order to overcome limitations of standard experimental designs and prioritization of media culture components, a systematic investigation was carried out using the PB design. In this investigation, the effects of different factors such as 2,4-D, putrescine, carbohydrate sources (maltose, sorbitol, and sucrose), silver nitrate, glutamine, and …
Improvement Of The In Vitro Regeneration And Agrobacterium-Mediated Genetic Transformation Of Medicago Sativa L., Farzad Nofouzi, Muhammet Çağri Oğuz, Saber Delpasand Khabbazi, Ali̇ Ergül
Improvement Of The In Vitro Regeneration And Agrobacterium-Mediated Genetic Transformation Of Medicago Sativa L., Farzad Nofouzi, Muhammet Çağri Oğuz, Saber Delpasand Khabbazi, Ali̇ Ergül
Turkish Journal of Agriculture and Forestry
Alfalfa is a fodder crop that accounts for one of the best sources of protein and is widely cultivated around the world. In vitro regeneration of alfalfa has been studied earlier; however, most of the studies were almost intervened with callus formation. In this study, 3 explant sources (cotyledonary node, hypocotyl, and root crown) of two Turkish cultivars (Nimet and Savaş) were excised from young seedlings. Explants were subjected to different concentrations of BAP, BAP-IBA, and TDZ to evaluate the direct in vitro regeneration potential of selected plant parts. Moreover, we transformed the alfalfa plant with pBin19 harboring 35s.GUS-INT_35s.nptII construct …
Genotypic-Unspecific Protocols For The Commercial Micropropagation Of Eucalyptus Grandis × Nitens And E. Grandis × Urophylla, Maria Paula Watt
Genotypic-Unspecific Protocols For The Commercial Micropropagation Of Eucalyptus Grandis × Nitens And E. Grandis × Urophylla, Maria Paula Watt
Turkish Journal of Agriculture and Forestry
Clones of Eucalyptus grandis × nitens and E. grandis × urophylla were subjected to various culture conditions at every culture stage leading to plantlet regeneration directly from axillary buds. The objective was to determine the possibility of using the same protocol for all clones. Although genotypic effects were evident, generally most clones responded similarly to the tested variations in each of the protocol stages, i.e. bud break, multiplication, and rooting. Estimated yields for the clones of one of the tested E. grandis × nitens hybrids ranged from 24 to 90 acclimatised plants per explant, but those of a natural hybrid …
In Vitro Screening Of Octoploid Fragaria Chiloensis And Fragaria Virginiana Genotypes Against Iron Deficiency, Ayfer Alkan Torun, Yildiz Kaçar, Belgi̇n Bi̇çen, Nazi̇fe Erdem, Sedat Serçe
In Vitro Screening Of Octoploid Fragaria Chiloensis And Fragaria Virginiana Genotypes Against Iron Deficiency, Ayfer Alkan Torun, Yildiz Kaçar, Belgi̇n Bi̇çen, Nazi̇fe Erdem, Sedat Serçe
Turkish Journal of Agriculture and Forestry
Iron (Fe) deficiency is one of the common problems in strawberry (Fragaria × ananassa Duch.) growing regions. In vitro screening enables researchers to evaluate genotype performance under several concentrations of Fe in a relatively fast and economical way. Therefore, we evaluated the responses of several genotypes belonging to octoploid Fragaria chiloensis (L.) Mill. and Fragaria virginiana Mill. (the progenitors of cultivated strawberry) against Fe treatments under in vitro conditions. In the first experiment, HM 1, Pigeon Point, CFRA1267 (F. chiloensis subsp. pacifica), and CA1541 (F. chiloensis subsp. chiloensis) were tested with various Fe concentrations (0%, 10%, 15%, 25%, 50%, 65%, …
Regeneration And Histological Analysis Of Snake Melon (Cucumis Melo Var. Flexuosus (L.) Naudin) By Direct Organogenesis, Yeşi̇m Yalçin Mendi̇, Selay Eldoğan, Rafael Gutakev, Muzaffer İpek, Pembe Çürük, Seli̇m Çeti̇ner
Regeneration And Histological Analysis Of Snake Melon (Cucumis Melo Var. Flexuosus (L.) Naudin) By Direct Organogenesis, Yeşi̇m Yalçin Mendi̇, Selay Eldoğan, Rafael Gutakev, Muzaffer İpek, Pembe Çürük, Seli̇m Çeti̇ner
Turkish Journal of Agriculture and Forestry
In vitro morphogenesis of Cucumis melo var. flexuosus (L.) Naudin was studied by direct organogenesis. Induction of adventitious buds was obtained from distal and proximal parts of the cotyledon incubated on Murashige and Skoog (1962) medium containing different concentrations of 6-benzyladenine (BA) (0.0, 0.5, 1.0, and 2.0 mg L^{-1}) and indole-3-acetic acid (IAA) (0.0, 0.1, and 0.5 mg L^{-1}). The best organogenesis was obtained from the proximal part of the cotyledon on medium containing 0.5 mg L^{-1} BA and 0.5 mg L^{-1} IAA (88%). This concentration was used for histological analysis. Less regeneration was obtained on media containing 1.0 mg …
In Vitro Propagation Of Some New Banana Types (Musa Spp.), Hami̇de Gübbük, Mustafa Pekmezci̇
In Vitro Propagation Of Some New Banana Types (Musa Spp.), Hami̇de Gübbük, Mustafa Pekmezci̇
Turkish Journal of Agriculture and Forestry
Three newly selected superior banana types were used to study the effects of different cytokinin and auxins on shoot multiplication and rooting. Benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] and thidiazuron [(TDZ) (0.4, 1, 2 and 3 µM)] were tested alone and with 1 µM indoleacetic acid (IAA) for the propagation stage. We compared basal Murashige and Skoog (MS) medium, active charcoal (5 g l^{-1}), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Shoot proliferation and elongation were significantly greater with TDZ than with BAP all 3 types. Furthermore, each cytokinin with IAA increased shoot proliferation and elongation …
In Vitro Plantlet Regeneration From Mature Embryos Of Linden (Tilia Platyphyllos Scop.) And Multiplication Of Its Buds, Ali̇ Ömer Üçler, Nuray Mollamehmetoğlu
In Vitro Plantlet Regeneration From Mature Embryos Of Linden (Tilia Platyphyllos Scop.) And Multiplication Of Its Buds, Ali̇ Ömer Üçler, Nuray Mollamehmetoğlu
Turkish Journal of Agriculture and Forestry
In this study, the effects of different sucrose, BAP and Kinetin concentrations in MS medium on plantlet formation, bud formation on the plantlets and development of embryos isolated from the seeds of Tilia platyphyllos under in vitro conditions were investigated. In addition, the effects of different culture times on the development of embryos and plantlet formation were studied. The results indicated that the cultured embryos on the media supplemented with sucrose (10, 20, 30, 40, 50 and 60 g/l) developed plantlets with epicotyls and roots. The best results were obtained on the medium supplemented with 30 g/l of sucrose. Epicotyls …