Forest Sciences Commons^™

Assessment Of Genetic Diversity And Search For Plum Pox Virus Resistance Alleles Of Apricot (Prunus Armeniaca L.) Genotypes Spread In Azerbaijan Using Ssr Markers, Ami̇na Raki̇da, Necati̇ Çeti̇nsağ, Zeynal Akparov, Logman Bayramov, Sevda Babayeva, Vusala Izzatullayeva, Mehraj Abbasov, Sezai̇ Erci̇şli̇, Kahraman Gürcan

Turkish Journal of Agriculture and Forestry

Apricot is one of the most important stone-fruit plants widely cultivated in Azerbaijan. SSR markers were used to evaluate the genetic diversity of 61 apricot genotypes representing the entire gene pool in Azerbaijan. A total of 138 alleles were produced based on 17 SSR markers with an average of 8.1 alleles per primer. The polymorphism information content (PIC) and expected (He) heterozygosity for individual loci were in the range of 0.42–0.8 (mean 0.68) and 0.52–0.82 (mean 0.72), respectively, indicating the abundant genetic variation in Azerbaijani apricot cultivars. The polymorphisms generated by SSRs were enough to differentiate 95% of genotypes, while …

Coping With Environmental Constraints: Geographically Divergent Adaptive Evolution And Germination Plasticity In The Transcontinental Populus Tremuloides, Roos Goessen, Nathalie Isabel, Christian Wehenkel, Nathalie Pavy, Lisa Tischenko, Lyne Touchette, Isabelle Giguère, Marie-Claude Gros-Louis, Jérôme Laroche, Brian Boyle, Raju Soolanayakanahally, Karen E. Mock, Javier Hernández-Velasco, Sergio Leonel Simental-Rodriguez, Jean Bousquet, Ilga M. Porth

Aspen Bibliography

Societal Impact Statement

Syntheses clearly show that global warming is affecting ecosystems and biodiversity around the world. New methods and measures are needed to predict the climate resilience of plant species critical to ecosystem stability, to improve ecological management and to support habitat restoration and human well-being. Widespread keystone species such as aspen are important targets in the study of resilience to future climate conditions because they play a crucial role in maintaining various ecosystem functions and may contain genetic material with untapped adaptive potential. Here, we present a new framework in support of climate-resilient revegetation based on comprehensively understood …

Determination Of Genetic Diversity, Sinigrin Contents, And Elicitors-Induced Enhancement Of Sinigrin In Nasturtium Officinale L., Sobia Zaman, Khadim Shah, Bakhat Zaman, Aziz Ullah Sayal, Syed Tatheer Alam Naqvi, Mohammad Maroof Shah, Jamshaid Hussain, Amjad Hassan, Arshad Mehmood Abbasi, Raza Ahmad

Turkish Journal of Agriculture and Forestry

Nasturtium officinale L. is a plant of significant medicinal importance. The effect of climatic variations on its genetic makeup and biochemistry has not been studied in Pakistan. Present study was conducted to evaluate genetic diversity and variations in sinigrin contents, an important anticancer metabolite, in the leaves of N. officinal collected from different districts of Pakistan including Muzaffarabad, Chilas, Jaglot, Dir, Swat, Abbottabad, and Haripur. Furthermore, the effects of exogenous abscisic acid (ABA), salicylic acid (SA), and sodium nitroprusside (SNP) on the contents of sinigrin and expression of 2-oxoacid-dependent dioxygenase (GSLOH) gene (TR14312) were also studied. Randomly amplified polymorphic DNA …

Multidimensional Scaling Analysis Of Sensory Characteristics And Quantitative Traits Inwild Apricots, Rabi̇a Albayrak Deli̇ali̇oğlu, Hati̇ce Dumanoğlu, Veli̇ Erdoğan, Sai̇d Efe Dost, Ali̇ Kesi̇k, Zahi̇de Kocabaş

Turkish Journal of Agriculture and Forestry

The Cappadocia Region in Central Anatolia, Turkey is rich in wild apricot genetic resources. In this study, a multivariate analysis method of metric and nonmetric multidimensional scaling analysis (MDS) was used to classify 43 wild apricot genotypes and 5 standard cultivars by using Euclidean distance. MDS was applied, based on dissimilarities, to quantitative traits of weight, height, width and thickness of fruit, stone and kernel weight, flesh/stone ratio, total soluble solids content, titratable acidity and pH in fruit juice, and sensory attributes of fruit size and shape, cavity depth, suture (cheek line), fruit apex, fruit attractiveness, skin pubescence, ground and …

Genetic Characterization Of Almond (Prunus Amygdalus L) Using Microsatellite Markersin The Area Of Adriatic Sea, Jasna Hasanbegovic, Semina Hadziabulic, Mirsad Kurtovic, Fuad Gasi, Biljana Lazovic, Boris Dorbic, Azra Skender

Turkish Journal of Agriculture and Forestry

The use of microsatellite (SSR) markers has successfully found its application in genetic characterization and examination of the origin of a large number of fruit species. Mediterranean germplasm is characterized by a great variety of almond genotypes. The study covered two geographically distant regions Montenegro (Bar) and Croatia (Sibenik) in a sample of 60 almond genotypes. Genetic analysis of almonds involved the use of ten microsatellite primers for genetic characterization of 60 examined genotypes, which successfully amplified PCR products and were highly polymorphic. Nine microsatellite markers used for the genetic characterization of almonds are derived from Prunus persica (UDP97-402, UDP98-411, …

Genetic Diversity Analysis In The Turkish Pepper Germplasm Using Ipbs Retrotransposonbasedmarkers, Mehtap Yildiz, Meti̇n Koçak, Muhammad Azhar Nadeem, Pablo Cavagnaro, Karina Barboza, Faheem Shahzad Baloch, Duygu Argün, Davut Keleş

Turkish Journal of Agriculture and Forestry

Capsicum is one of the most important and diverse plant taxa, widely used as a spice and vegetable worldwide, including Turkey. Germplasm characterization is an essential step for crop breeding. In the present study, we characterized the genetic diversity and population structure of a collection of 94 pepper accessions using inter-primer binding site (iPBS) retrotransposon-based markers. A total of 20 iPBS primers were used that generated 172 bands (mean = 8.6 bands/primer), of which ~92% were polymorphic in the entire germplasm collection, whereas 83%, 69%, and 80% of the bands were polymorphic within the C. annuum, C. chinense, and C. …

Genetic Diversity Among Melon (Cucumis Melo L.) Accessionsrevealed By Morphological Traits And Issr Markers, Niyazi Guliyev, Saida Sharifova, Javid Ojaghi, Mehraj Abbasov, Zeynal Akparov

Turkish Journal of Agriculture and Forestry

The genetic relationships among 48 melon (Cucumis melo L.) genotypes collected from various parts of Azerbaijan were determined by comparing their phenotypic and molecular traits. Eleven agromorphological traits and 10 polymorphic inter-simple sequence repeat (ISSR) primers were used to define the genetic diversity. Principal component analysis grouped the agromorphological traits into the first four axes, describing 78% of the total variations. The highest genetic variation coefficient was found for yield per hectare (20.32%) and for fruit length (17.35%). Calculated heritability for yield was 0.96. The analysis of morphological traits grouped the accessions into four clusters. The 10 ISSR primers yielded …

Genetic Diversity Assessment In Nicotiana Tabacum L. With Ipbs-Retrotransposons, Gülsüm Yaldiz, Mahmut Çamlica, Muhammad Azhar Nadeem, Muhammad Amjad Nawaz, Faheem Shahzad Baloch

Turkish Journal of Agriculture and Forestry

Knowledge of genetic diversity could be beneficial by contributing important information in the selection of breeding material. The objective of this study was to explore the genetic diversity and relationship in the Turkish tobacco germplasm with the iPBSretrotransposons marker that emerged as a universal method. A total of 90 landraces and 6 commercial cultivars collected from different geographical regions of Turkey were used in this study. Eleven most polymorphic iPBS-retrotransposons primers yielded a total of 119 scorable bands and 98 of these bands were found to be polymorphic (82.35%), with an average of 8.91 polymorphic fragments for each primer. The …

Highly Informative Ssr Genotyping Reveals Large Genetic Diversity And Limited Differentiation In European Larch (Larix Decidua) Populations From Romania, Pietro Gramazio, Ioana M. Plesa, Alina M. Truta, Adriana F. Sestras, Santiago Vilanova, Mariola Plazas, Oscar Vicente, Monica Boscaiu, Jaime Prohens, Radu E. Sestras

Turkish Journal of Agriculture and Forestry

European larch (Larix decidua) is naturally distributed at high elevations in Central Europe. Romanian populations of L. decidua are scattered in different areas of the country. In this study, we used 12 highly informative genomic simple sequence repeat (SSR) markers for genotyping seven populations from different areas of Romania. The SSR markers were highly variable, with up to 11 alleles per SSR locus and an average polymorphic information content of 0.713. High values of observed (Ho = 0.542) and expected (He = 0.738) heterozygosities were observed. Cluster analysis of populations did not group them according to geographical distance, but some …

Evaluation Of The Genetic Diversity Of Pomegranate Accessions From Turkey Using New Microsatellite Markers, Oğuzhan Çalişkan, Safder Bayazit, Mert Öktem, Ali̇ Ergül

Turkish Journal of Agriculture and Forestry

Turkey has many valuable genetic resources for pomegranate; however, there have not been many studies on the identification and characterization of these important genetic resources. New microsatellite markers were used to characterize a set of 78 pomegranate accessions from Turkey. Using six SSR primers, a total of 41 alleles were characterized with an average of 4.6 alleles per locus and mean probability of identity (PI) value of 0.366. These data indicated a high level of polymorphism in pomegranate germplasm. Five synonymous groups could be detected among 30 accessions. This microsatellite-based key is a first step towards a database for marker-assisted …

Diversity Analysis Of Genetic, Agronomic, And Quality Characteristics Of Bread Wheat (Triticum Aestivum L.) Cultivars Grown In Turkey, Mustafa Erayman, Emre İlhan, Abdi̇l Hakan Eren, Hüseyi̇n Güngör, Batuhan Akgöl

Turkish Journal of Agriculture and Forestry

Turkey is an important bread wheat producer. The objective of this study was to dissect the diversity of genetic, agronomic, and quality characteristics of bread wheat cultivars grown on 25% of the total wheat area in Turkey. A total of 24 wheat cultivars and 5 wild progenitors of wheat were examined using 24 simple sequence repeat (SSR) primers with a known physical locus on the A, B, and D genomes of hexaploid wheat. A total of 72 bands produced 939 alleles on the wheat cultivars and wild progenitors. Markers were efficient in discriminating the species and the highest genetic diversity …

Diversity Analysis Of Green Gram (Vigna Radiata (L.) Wilczek)Through Morphological And Molecular Markers, Gunjeet Kaur, Arunabh Joshi, Devendra Jain, Ravish Choudhary, Divya Vyas

Turkish Journal of Agriculture and Forestry

Twenty-three genotypes of green gram (Vigna radiata (L.) Wilczek) were collected to determine the extent of genetic diversity through morphological characters as well as random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and simple sequence repeat (SSR) fingerprinting results. Analysis of variance revealed significant mean square values that indicated a substantial degree of genetic variability among the genotypes. Significant genetic variance was observed for days to 50% maturity, days to maturity, plant height (cm), number of branches/plant, number of pods/plant, pod length (cm), 1000-seed weight (g), seed yield/plant (g), biological yield (g), and harvest index (%) during cultivation in …

Molecular Markers To Evaluate Genetic Diversity Among Venturia Inaequalis Isolates Obtained From Apple Plantations In Isparta Province, Suat Kaymak, Nuh Boyraz, Jon Daniels

Turkish Journal of Agriculture and Forestry

Turkey, the third leading apple producer in world, produces approximately 2.6 million tons of apples per year. Venturia inaequalis, the causal agent of apple scab, is a major fungal disease among apples varieties. This study was conducted in accordance with the plant protection method for isolates collected from the apple production areas in Isparta Province. The purpose of this study was to determine genetic differences among V. inequalis isolates causing apple scab, and to discriminate the effects of varieties and geographical origins on genetic diversity. For this purpose, a total of 83 samples from the leaves and fruits of infected …

Diversity Pattern Among Agromorphological Traits Of The Swiss Chard(Beta Vulgaris L. Subsp. Vulgaris) Genetic Resources Of Turkey, Mehmet Kadri̇ Bozokalfa, Dursun Eşi̇yok, Tansel Kaygisiz Aşçioğul

Turkish Journal of Agriculture and Forestry

Swiss chard (Beta vulgaris L. subsp. vulgaris) is a nutritionally rich leafy vegetable of moderate economic value. Despite being the first species among the genus Beta to be cultivated, knowledge regarding its biodiversity and agronomic and morphological properties is limited. The objective of this study was to evaluate the agromorphological characteristics of the Turkish Swiss chard genetic resources, and to determine their genetic relationships and diversity. To achieve this, a total of 52 Swiss chard accessions from a wide range of environmental and geographical origins in Turkey and two cultivars (one local and one foreign) were investigated. Fourteen qualitative and …

Evaluation Of Drought Tolerance Of Winter Bread Wheat Genotypes Underdrip Irrigation And Rain-Fed Conditions, Jamala Mursalova, Zeynal Akparov, Javid Ojaghi, Mahammad Eldarov, Savaş Belen, Nurberdi Gummadov, Alexey Morgounov

Turkish Journal of Agriculture and Forestry

To evaluate the genetic diversity in 48 winter bread wheat genotypes originating from different countries, an experiment based on randomized complete block design with 4 replications was carried out in drip irrigation and rain-fed conditions at the field station of the Transitional Zone Agricultural Research Institute, Eskişehir, Turkey in 2011 and 2012. Based on grain yield under rain-fed and irrigated conditions, drought tolerance indices, i.e. stress susceptibility index (SSI), stress tolerance index (STI), tolerance index (TOL), mean productivity (MP), and geometric mean productivity (GMP), were calculated to identify genotypes with better yield and drought tolerance. Analysis of variance indicated that …

Genetic Diversity And Chemical Characterization Of Selected Polish And Russian Cultivars And Clones Of Blue Honeysuckle (Lonicera Caerulea), Elzbieta Kaczmarska, Jacek Gawronski, Magdalena Dyduch-Sieminska, Agnieszka Najda, Wojciech Marecki, Jadwiga Zebrowska

Turkish Journal of Agriculture and Forestry

In traditional medicine, Lonicera caerulea has a notable place, and it has been used for thousands of years in East Asian countries for treating cancer, inflammation, hepatic complications, influenza, and wounds. However, the molecular and chemical characterization of this plant is not well defined. In this study, inter-simple sequence repeat (ISSR) markers were used to determine the genetic variability of 7 blue honeysuckle cultivars and 3 clones. To characterize these 10 accessions, total flavonoids, phenolic acids contents, and antioxidant activities were estimated. Twelve primers generated 101 polymorphic ISSR-polymerase chain reaction bands. Based on the ISSR amplification profiles, a cluster dendrogram …

Identification And Functional Annotation Of Expressed Sequencetags Based Ssr Markers Of Stevia Rebaudiana, Rajinder Kaur, Neha Sharma, Ravinder Raina

Turkish Journal of Agriculture and Forestry

Stevia rebaudiana Bertoni, a perennial herb belonging to the family Asteraceae, is a tropical as well as subtropical plant of medicinal importance. Despite being an important medicinal plant and in fact proving a boon to diabetics, it has remained almost ignored by molecular biologists. A systematic molecular evaluation of stevia germplasm and molecular breeding work are sparsely reported mainly due to the limited specific molecular markers available. Expressed sequence tags (ESTs) are a valuable resource for developing simple sequence repeats (SSRs). In this study, 5548 stevia ESTs sequences from leaf tissues were retrieved from the NCBI database. Clustering and assembly …

Genetic Variability And Relationship Studies In New Indian Papaya (Carica Papaya L.) Germplasm Using Morphological And Molecular Markers, Parmeshwar Lal Saran, Ravish Choudhary, Ishwar Singh Solanki, Pravin Patil, Sanjay Kumar

Turkish Journal of Agriculture and Forestry

Papaya germplasm showed wide morphological diversity in terms of fruit yield, weight, length, cavity, fruiting zone, flesh thickness, flesh color, and total soluble solids content. RAPD and ISSR markers also showed polymorphisms at rates of of 84.32% and 84.67%, respectively. Jaccard's genetic similarity values of RAPD and ISSR were found in the range of 0.30-0.99 (average: 0.65) and 0.26-0.95 (average: 0.61), respectively, suggesting a moderate level of genetic diversity within the papaya group. Seven of the 15 RAPD primers and 5 of the 11 ISSR primers were also able to generate cultivar-specific amplicons, which were used for identification of germplasms …

Comparative Analysis Of Genetic Diversity Among Chinese Watermelon Germplasmsusing Ssr And Srap Markers, And Implications For Future Genetic Improvement, Pangqiao Wang, Qiong Li, Jianbin Hu, Yan Su

Turkish Journal of Agriculture and Forestry

The genetic diversity of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] in China, the world's largest producer of watermelon fruits, has not been examined. Two molecular markers, sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR), were used to investigate the genetic variation and genetic relationship among 54 Chinese watermelon accessions, as well as 7 accessions from Africa, the United States, and Japan. SRAP assay generated 312 bands, of which 163 were polymorphic, while SSR assay detected 101 alleles with 87 polymorphic alleles and 114 genotypes. A comparative analysis of the diversity index, effective multiplex ratio, and marker index of …

Genetic Diversity And Population Structure Of Common Bean (Phaseolus Vulgaris L.) Accessions Through Retrotransposon-Based Interprimer Binding Sites (Ipbss) Markers, Seda Nemli̇, Tala Kianoosh, Muhammed Bahatti̇n Tanyolaç

Turkish Journal of Agriculture and Forestry

The common bean (Phaseolus vulgaris L.) is an important crop throughout the world and is highly recommended for many developing countries due to its nutritional components. A retrotransposon-based DNA marker system, interprimer binding sites (iPBSs), developed from reverse transcriptase primer binding sites, was used to determine genetic diversity in common bean accessions. The genetic polymorphism and effectiveness of the marker system were evaluated. A total of 180 polymorphic bands were detected using 47 iPBS primers among 67 common bean accessions, with an average of 4 polymorphic fragments per primer. The genetic similarity between accessions was calculated using the software NTSYS-pc …

Genetic Comparison Between Natural And Planted Populations Of Pinus Brutia And Cupressus Sempervirens In Syria, Batoul N. Al-Hawija, Viktoria Wagner, Isabell Hensen

Turkish Journal of Agriculture and Forestry

There is wide consensus that ongoing deforestation contributes to global warming and poses a threat to species diversity. Less understood is whether the practice of creating plantations might also erode genetic diversity and undermine the genetic structure of tree populations. We tested these hypotheses in natural and planted populations of Pinus brutia Ten. subsp. brutia and Cupressus sempervirens L. var. horizontalis (Mill.), 2 important forestry species in the Mediterranean region. We used plant material from 3 different bioclimatic regions in Syria. Using RAPD markers, we evaluated the genetic diversity and structure of 12 populations of P. brutia (6 natural, 6 …

Assaying Of Diversity Among Soybean (Glycin Max (L.) Merr.) And Peanut (Arachis Hypogaea L.) Genotypes At Dna Level, Faheem Shehzad Baloch, Cemal Kurt, Hali̇l Hali̇s Arioğlu, Hakan Özkan

Turkish Journal of Agriculture and Forestry

Developing successful strategies to ensure future increase in yield of soybean and peanut crops hinges in part, on improving the genetic basis of the cultivars. Knowledge of genetic relationships in crop breeding programs provides valuable information that can be used by plant breeders as a parental line selection tool. So far, a thorough analysis of genetic diversity among the soybean and peanut genotypes grown in Turkey had not been attempted at DNA level. In this study, inter-simple sequence repeats (ISSR) and sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity between 21 soybean and 32 peanut cultivars …

Genetic Diversity Of Wilt And Root Rot Pathogens Of Chickpea, As Assessed By Rapd And Issr, Harun Bayraktar, F. Sara Dolar

Turkish Journal of Agriculture and Forestry

Intra- and interspecific polymorphisms among fungal pathogens that cause wilt and root rot on chickpea were investigated by using 30 RAPD (random amplified polymorphic DNA) and 20 ISSR (inter-simple sequence repeats) primers. UPGMA (unweighted pair group method with arithmetic average) cluster analysis of RAPD and ISSR datasets using Dice's coefficient differentiated all fungal isolates from each other and revealed considerable genetic variability between the isolates. The isolates that were not easily separable using morphological characteristics were classified again according to their branching in dendrograms. A partial correlation was observed between cluster analysis and geographic origin of Fusarium solani isolates. Additionally, …

Molecular Characterization And Genetic Diversity Analysis Of Citrus Cultivars By Rapd Markers, M. N. R. Baig, Sapna Grewal, Santosh Dhillon

Turkish Journal of Agriculture and Forestry

Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers; of which 231 were polymorphic and some species or cultivar specific RAPD markers. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated Jatti-Khatti from all major clusters at a similarity coefficient of 0.61. The average genetic similarity value observed across all the genotypes was 0.63, with the 2 sweet orange cultivars, Jaffa …