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Full-Text Articles in Forest Sciences
Highly Polymorphic Novel Simple Sequence Repeat Markers From Class I Repeats Inwalnut (Juglans Regia L.), Emre Eser, Hayat Topçu, Sina Kefayati, Mehmet Sütyemez, Md Rashedul Islam, Sali̇h Kafkas
Highly Polymorphic Novel Simple Sequence Repeat Markers From Class I Repeats Inwalnut (Juglans Regia L.), Emre Eser, Hayat Topçu, Sina Kefayati, Mehmet Sütyemez, Md Rashedul Islam, Sali̇h Kafkas
Turkish Journal of Agriculture and Forestry
Walnut (Juglans regia L.) is the most important species in the genus Juglans due to the high commercial value of its nuts and timber. Simple sequence repeats (SSRs) are considered as the markers of choice owing to their codominant nature. Since very few SSRs have been developed in walnut, this study aimed to develop numerous polymorphic SSRs from Class I repeats by using DNA sequences of the Chandler cultivar. In all, 800 SSRs were designed and tested in 8 Turkish, 3 French, and 5 US walnut cultivars, of which 88 (11%) did not produce bands, 161 (20.1%) were monomorphic, and …
Molecular Characterization Of Apple (Malus × Domestica Borkh.) Genotypes Originatingfrom Three Complementary Conservation Strategies, Anca Butiuc, Ana Coste, Anca Farkas, Victoria Cristea, Valentina Isac, Adela Halmagyi
Molecular Characterization Of Apple (Malus × Domestica Borkh.) Genotypes Originatingfrom Three Complementary Conservation Strategies, Anca Butiuc, Ana Coste, Anca Farkas, Victoria Cristea, Valentina Isac, Adela Halmagyi
Turkish Journal of Agriculture and Forestry
Apple (Malus × domestica Borkh.) genotypes originating from different plant collections (field collection, in vitro plant collections undergoing or not undergoing cryopreservation) were screened and characterized by SSR markers. Shoot tips excised from plants grown in vitro were successfully cryopreserved by encapsulation-dehydration. The highest regrowth frequency (69%, cultivar Goldrush) of cryopreserved apices was achieved after 24 h of osmoprotection in 0.5 M sucrose, 3 h of desiccation, and 24% water content of alginate beads. No differences in morphological characteristics including shoot length and number and length of roots were observed between controls and plants recovered after cryopreservation. SSR markers were …