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Full-Text Articles in Forest Sciences
Pokeweed (Phytolacca Americana L.) Antiviral Protein Inhibits Zucchini Yellow Mosaic Virus Infection In A Dose-Dependent Manner In Squash Plants, Hi̇kmet Murat Si̇pahi̇oğlu, İlhan Kaya, Mustafa Usta, Murat Ünal, Di̇lek Özcan, Meryem Özer Di̇lmen, Abdullah Güller, Vicente Pallas
Pokeweed (Phytolacca Americana L.) Antiviral Protein Inhibits Zucchini Yellow Mosaic Virus Infection In A Dose-Dependent Manner In Squash Plants, Hi̇kmet Murat Si̇pahi̇oğlu, İlhan Kaya, Mustafa Usta, Murat Ünal, Di̇lek Özcan, Meryem Özer Di̇lmen, Abdullah Güller, Vicente Pallas
Turkish Journal of Agriculture and Forestry
Pokeweed antiviral protein (PAP) of Phytolacca americana L. (pokeweed) is a single-chain ribosome-inactivating protein (RIP) characterized by its ability to depurinate plant ribosomes. Here, we isolated, cloned, and expressed the ribosome inactivating protein (RIP) gene, designated as pokeweed antiviral protein type 1 (PAP I), from the summer leaves of pokeweed collected from the Black Sea region (Turkey). Our findings presented here provide direct evidence that exogenous application of PAP I causes concentration-dependent inhibition of Zucchini yellow mosaic virus (ZYMV) infection on squash plants. Squash plants were exposed to PAP I protein with and without DMSO for four consecutive days. Regular …