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Developing Tobacco Lines Resistant To Powdery Mildew (Erysiphe Cichoracearum L.) By Anther Culture Technique For The Aegean Region, A. Sani̇ye Gencer

Turkish Journal of Agriculture and Forestry

The anther culture technique as a biotechnological application was combined with conventional breeding methods in order to improve tobacco varieties' resistant to powdery mildew prevailing in the Aegean region of Turkey. For this, resistance was transferred to two varieties from a genitor by the backcross method leading to derive haploid plants from the cultured anthers of BC_1 plants firstly, and dihaploid plants by acenaphthene or colchicine treatments secondly. A total of 14 dihaploid lines out of 67 DH lines developed were found to be resistant to powdery mildew in disease screening tests. They were later subjected to field experiments to …