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Full-Text Articles in Forest Sciences
Refining A Protocol For Somatic Embryogenesis And Plant Regeneration Of Phalaenopsis Amabilis Cv. Jinan From Mature Tissues, Reza Ghahremani, Shirin Dianati Daylami, Masoud Mirmasoumi, Naser Askari, Kourosh Vahdati
Refining A Protocol For Somatic Embryogenesis And Plant Regeneration Of Phalaenopsis Amabilis Cv. Jinan From Mature Tissues, Reza Ghahremani, Shirin Dianati Daylami, Masoud Mirmasoumi, Naser Askari, Kourosh Vahdati
Turkish Journal of Agriculture and Forestry
Phalaenopsis is one of the most remarkable commercial genera in Orchidaceae family. Using immature explants for tissue culture of orchid results in undesired characteristics such as genetic variation and long-lasting juvenility. In this study, we optimized a protocol for somatic embryogenesis of Phalaenopsis amabilis cv. Jinan, by using mature thin cell layer (TCL) explants to minimize immature explant limitations. We examined somatic embryogenesis from two types of mature explants, viz. leaf lTCL and flower stalk nodes tTCL at various concentrations of thidiazuron (TDZ) (0, 1, 2, and 3 mg L-1). The results demonstrated that lTCL explants cultured on medium supplemented …