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Full-Text Articles in Forest Sciences
Identification Of Genetic Diversity Among Mutant Lemon And Mandarin Varieties Usingdifferent Molecular Markers, Görkem Sülü, Yildiz Kaçar, İlknur Polat, Aytül Ki̇tapci, Ertuğrul Turgutoğlu, Özhan Şi̇mşek, Gül Satar
Identification Of Genetic Diversity Among Mutant Lemon And Mandarin Varieties Usingdifferent Molecular Markers, Görkem Sülü, Yildiz Kaçar, İlknur Polat, Aytül Ki̇tapci, Ertuğrul Turgutoğlu, Özhan Şi̇mşek, Gül Satar
Turkish Journal of Agriculture and Forestry
Mandarin (Citrus reticulate Blanco) and lemon (C.limon L.) are among the most cultivated Citrus species in the world. Among different approaches, gamma radiation is widely used to induce mutations in citrus breeding studies. Gamma radiation causes DNA damage, randomly inducing several mutations in the genome. Molecular techniquesarewidely used to detect such induced mutations and genetic diversity in plants.In this study, simple sequence repeats (SSR), inter-simple sequence repeat (ISSR) and single-strand conformation polymorphism (SSCP) markers were used to detectthe induced mutations andgenetic diversityin someirradiated mandarin and lemon genotypes in comparison with standard citrus varieties. The irradiated genotypes used in this study …
Efficient Protoplast Isolation From Ovule-Derived Embryogenic Callus In Citrus Volkameriana, Berken Çi̇men
Efficient Protoplast Isolation From Ovule-Derived Embryogenic Callus In Citrus Volkameriana, Berken Çi̇men
Turkish Journal of Agriculture and Forestry
The present study reports on the isolation of viable protoplast from ovule-derived embryogenic calli of Volkameriana (Citrus volkameriana L.), which is a rootstock in high demand for lemon production.Ovules of C. Volkameriana isolated at 3 different immature fruit stages, comprising4, 8, and 12 weeks after anthesis (WAA), were cultured on5 different media in order to produce embryogenic callus lines as a source material for protoplast isolation. EME medium (MT basal medium + 0.5 gL-1 malt extract), with the addition of phytohormones [kinetin (KIN), 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP)] at different concentrations, were tested for callogenesis. According to 2-way ANOVA, …